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Front Matter: Volume 11553 前题:卷11553
Optics in Health Care and Biomedical Optics X Pub Date : 2020-11-09 DOI: 10.1117/12.2585933
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引用次数: 0
Structured illumination microscopy using digital micromirror device and coherent light source 采用数字微镜装置和相干光源的结构照明显微术
Optics in Health Care and Biomedical Optics X Pub Date : 2019-10-30 DOI: 10.1063/5.0008264
Meiqi Li, Yaning Li, Wenhui Liu, A. Lal, Shan Jiang, D. Jin, Houpu Yang, Shu Wang, Karl Zhanghao, P. Xi
{"title":"Structured illumination microscopy using digital micromirror device and coherent light source","authors":"Meiqi Li, Yaning Li, Wenhui Liu, A. Lal, Shan Jiang, D. Jin, Houpu Yang, Shu Wang, Karl Zhanghao, P. Xi","doi":"10.1063/5.0008264","DOIUrl":"https://doi.org/10.1063/5.0008264","url":null,"abstract":"Structured illumination microscopy (SIM) achieves doubled spatial resolution by exciting the specimen with a high-contrast, high-frequency sinusoidal pattern. Such an excitation pattern can be generated by interference between multiple laser beams, which are diffracted from a grating. In SIM, 2D imaging requires 9 patterns and 3D imaging requires 15 patterns. Compared to mechanical movement of gratings, opti-electro devices provide rapid switch of the excitation patterns, in which Digital Micro-mirror Device (DMD) is most common in industry. Here we model DMD as the blazed grating and report a fast and cost-efficient SIM. Our home-built DMD-based laser interference structured illumination microscopy (DMD-ISIM) system reveals the nuclear pore complex and microtubule in mammalian cells with doubled spatial resolution. We further proposed multi-color DMD-ISIM system with simulation, which could potentially exploit the full power of DMD-ISIM.","PeriodicalId":404411,"journal":{"name":"Optics in Health Care and Biomedical Optics X","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122204241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 28
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