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Constitutive Expression of GATA4 Dramatically Increases the Cardiogenic Potential of D3 Mouse Embryonic Stem Cells. 组成性表达GATA4显著增加D3小鼠胚胎干细胞的心源性潜能。

Open Biotechnology Journal Pub Date : 2016-01-01 Epub Date: 2016-06-30 DOI: 10.2174/1874070701610010248

Lillian L Laemmle, Justus B Cohen, Joseph C Glorioso

{"title":"Constitutive Expression of GATA4 Dramatically Increases the Cardiogenic Potential of D3 Mouse Embryonic Stem Cells.","authors":"Lillian L Laemmle, Justus B Cohen, Joseph C Glorioso","doi":"10.2174/1874070701610010248","DOIUrl":"https://doi.org/10.2174/1874070701610010248","url":null,"abstract":"The transcription factor GATA binding protein 4 (GATA4) is a vital regulator of cardiac programming that acts by inducing the expression of many different genes involved in cardiomyogenesis. Here we generated a D3 mouse embryonic stem cell line that constitutively expresses high levels of GATA4 and show that these cells have dramatically increased cardiogenic potential compared to an eGFP-expressing control cell line. Embryoid bodies (EB) derived from the D3-GATA4 line displayed increased levels of cardiac gene expression and showed more abundant cardiomyocyte differentiation than control eGFP EB. These cells and two additional lines expressing lower levels of GATA4 provide a platform to screen previously untested cardiac genes and gene combinations for their ability to further increase the efficiency of cardiomyocyte differentiation beyond that achieved by transgenic GATA4 alone. Non-integrative delivery of identified gene combinations will aid in the production of differentiated cells for the treatment of ischemic cardiomyopathy.","PeriodicalId":39120,"journal":{"name":"Open Biotechnology Journal","volume":"10 ","pages":"248-257"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/72/56/nihms800739.PMC4948750.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34591073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Short Peptides as Inhibitors of Amyloid Aggregation. 短肽作为淀粉样蛋白聚集的抑制剂。

Open Biotechnology Journal Pub Date : 2011-12-23 DOI: 10.2174/1874070701105010039

Bradley Neddenriep, Anastasia Calciano, Daniel Conti, Erin Sauve, Marissa Paterson, Edward Bruno, David A Moffet

引用次数: 41

Synthesis and Purification of Highly Hydrophobic Peptides Derived from the C-Terminus of Amyloid β-Protein. 淀粉样β-蛋白c端高疏水肽的合成与纯化。

Open Biotechnology Journal Pub Date : 2008-01-01 DOI: 10.2174/1874070700802010087

M M Condron, B H Monien, G Bitan

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引用次数: 0