Open Biochemistry Journal最新文献

The Activity of α-glucosidase Inhibition of Pediococcus Acidilactici BAMA 4 Isolated from “Naniura” Traditional Foods from North Sumatera, Indonesia 印尼北苏门答腊“Naniura”传统食品中嗜酸性Pediococcus BAMA 4 α-葡萄糖苷酶抑制活性的研究

Open Biochemistry Journal Pub Date : 2023-09-25 DOI: 10.2174/1874091x-v17-230921-2023-2

Dewi Riastawaty, Ermi Girsang, Edy Fachrial, Chrismis Novalinda Ginting, Finna Piska, Ali Napiah Nasution

{"title":"The Activity of α-glucosidase Inhibition of Pediococcus Acidilactici BAMA 4 Isolated from “Naniura” Traditional Foods from North Sumatera, Indonesia","authors":"Dewi Riastawaty, Ermi Girsang, Edy Fachrial, Chrismis Novalinda Ginting, Finna Piska, Ali Napiah Nasution","doi":"10.2174/1874091x-v17-230921-2023-2","DOIUrl":"https://doi.org/10.2174/1874091x-v17-230921-2023-2","url":null,"abstract":"Background: Type 2 diabetes is caused by unhealthy lifestyles, such as consuming foods rich in simple sugars and lack of exercise. One of the treatment therapies for this disease is α-glucosidase inhibitors. Some strains of the lactic acid bacteria (LAB) group typically exhibit α-glucosidase inhibitory activity, antimicrobial activity, acids and bile salt tolerance, and probiotic status. Objective: This study aims to isolate LAB from naniura, characterize and test its activity as an α-glucosidase inhibitor, as well as identify those with the highest activity. Methods: The method used to molecularly identify potentially good LAB was through the amplification of the 16S rRNA gene. Results: This study obtained a total of 9 strains using BAMA codes 1, 2, 3, 4, 5, 6, 7, 8, and 9. The cocci cell activity, gram-positive, and antibacterial activity of BAMA 4 against E.coli was 7.54 mm, while against S. aureus was 8.05 mm. The percentage of viability in acid and bile salt is 28.7% and 68.6%, respectively. Additionally, the proportion of α-glucosidase inhibition is 65%. Discussion: The BAMA 4 strain is a species of Pediococcus acidilactici . Pediococcus is a genus of gram-positive lactic acid bacteria in the Lactobacillacea family. Conclusion: BAMA 4 strain produced antibacterial optimally and its cells survived the growth in acid and bile conditions. The percentage of probiotics was relevantly high in this activity. This study will be helpful for other in-vivo research.","PeriodicalId":38958,"journal":{"name":"Open Biochemistry Journal","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135924361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitric oxide and its metabolites in the critical phase of illness: rapid biomarkers in the making. 疾病关键阶段的一氧化氮及其代谢物:快速生物标志物的形成。

Open Biochemistry Journal Pub Date : 2013-01-01 Epub Date: 2013-03-08 DOI: 10.2174/1874091X01307010024

Asad I Mian, Mayank Aranke, Nathan S Bryan

{"title":"Nitric oxide and its metabolites in the critical phase of illness: rapid biomarkers in the making.","authors":"Asad I Mian, Mayank Aranke, Nathan S Bryan","doi":"10.2174/1874091X01307010024","DOIUrl":"https://doi.org/10.2174/1874091X01307010024","url":null,"abstract":"The potential of nitric oxide (NO) as a rapid assay biomarker, one that could provide a quantum leap in acute care, remains largely untapped. NO plays a crucial role as bronchodilator, vasodilator and inflammatory mediator. The main objective of this review is to demonstrate how NO is a molecule of heavy interest in various acute disease states along the emergency department and critical care spectrum: respiratory infections, central nervous system infections, asthma, acute kidney injury, sepsis, septic shock, and myocardial ischemia, to name just a few. We discuss how NO and its oxidative metabolites, nitrite and nitrate, are readily detectable in several body compartments and fluids, and as such they are associated with many of the pathophysiological processes mentioned above. With methods such as high performance liquid chromatography and chemiluminescence these entities are relatively easy and inexpensive to analyze. Emphasis is placed on diagnostic rapidity, as this relates directly to quality of care in acute care situations. Further, a rationale is provided for more bench, translational and clinical research in the field of NO biomarkers for such settings. Developing standard protocols for the aforementioned disease states, centered on concentrations of NO and its metabolites, can prove to revolutionize diagnostics and prognostication along a spectrum of clinical care. We present a strong case for developing these biomarkers more as point-of-care assays with potential of color gradient test strips for rapid screening of disease entities in acute care and beyond. This will be relevant to global health.","PeriodicalId":38958,"journal":{"name":"Open Biochemistry Journal","volume":" ","pages":"24-32"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2174/1874091X01307010024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40235081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Protein Kinase A Subunit α Catalytic and A Kinase Anchoring Protein 79 in Human Placental Mitochondria. 人胎盘线粒体蛋白激酶A亚基α催化和A激酶锚定蛋白79。

Open Biochemistry Journal Pub Date : 2012-01-01 Epub Date: 2012-04-11 DOI: 10.2174/1874091X01206010023

Maggie Pui Chi Ma, Murray Thomson

{"title":"Protein Kinase A Subunit α Catalytic and A Kinase Anchoring Protein 79 in Human Placental Mitochondria.","authors":"Maggie Pui Chi Ma, Murray Thomson","doi":"10.2174/1874091X01206010023","DOIUrl":"https://doi.org/10.2174/1874091X01206010023","url":null,"abstract":"Components of protein phosphorylation signalling systems have been discovered in mitochondria and it has been proposed that these molecules modulate processes including oxidative phosphorylation, apoptosis and steroidogenesis. We used electrophoresis and Western blots probed with specific antibodies to protein kinase A α catalytic subunit (PKAα Cat) and A kinase anchoring protein of approximately 79 kDa molecular weight (AKAP79) to demonstrate the presence of these two proteins in human placental mitochondria. Heavy mitochondria characteristic of cytotrophoblast were separated from light mitochondria characteristic of syncytiotrophoblast by centrifugation. PKAα Cat and AKAP79 were present in both heavy and light mitochondria with no significant difference in concentration. Sucrose density gradient separation of submitochondrial fractions indicated PKAα Cat is located predominantly in the outer membrane whereas AKAP79 is present mainly in the contact site fractions. These data indicate that PKAα Cat is present in the cytoplasm, nucleus and mitochondria of placental cells. AKAP79 is also present in human placental mitochondria but there may be anchoring proteins other than AKAP79 responsible for fixing PKA to the outer membrane. PKA may play roles in mitochondrial protein phosphorylation systems in both cytotrophoblast and syncytiotrophoblast.","PeriodicalId":38958,"journal":{"name":"Open Biochemistry Journal","volume":" ","pages":"23-30"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/03/82/TOBIOCJ-6-23.PMC3330370.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40179300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

High G+C Content of Herpes Simplex Virus DNA: Proposed Role in Protection Against Retrotransposon Insertion. 单纯疱疹病毒DNA的高G+C含量:对反转录转座子插入的保护作用

Open Biochemistry Journal Pub Date : 2007-12-04 DOI: 10.2174/1874091X00701010033

Jay C Brown

{"title":"High G+C Content of Herpes Simplex Virus DNA: Proposed Role in Protection Against Retrotransposon Insertion.","authors":"Jay C Brown","doi":"10.2174/1874091X00701010033","DOIUrl":"https://doi.org/10.2174/1874091X00701010033","url":null,"abstract":"The herpes simplex virus dsDNA genome is distinguished by an unusually high G+C nucleotide content. HSV-1 and HSV-2, for instance, have GC contents of 68% and 70% respectively, while that of the host (human) genome is 41%. To determine how GC content varies with genome location, GC content was measured separately in coding and intergenic regions of HSV-1 DNA. The results showed that the 75 genes constitute a uniform population with a mean GC content of 66.9 +/- 4.1%. In contrast, intergenic regions were found in two non-overlapping populations, one with a mean GC content (69.3 +/- 4.6% n=32) similar to the coding regions and another where the GC content is lower (56.0 +/- 4.9 n=30). Compared to other regions of the genome, intergenic regions with reduced GC content were found to be enriched in local GC minima, CACACA sequences and a primary target sequence (TTAAAA) for retrotransposition events. The results are interpreted to suggest that a high GC content is part of the way HSV-1 protects its genes from invasion by mobile genetic elements active during cell differentiation in the nervous system.","PeriodicalId":38958,"journal":{"name":"Open Biochemistry Journal","volume":" ","pages":"33-42"},"PeriodicalIF":0.0,"publicationDate":"2007-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f8/be/TOBIOCJ-1-33.PMC2606590.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28256648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Lipid rafts and caveolae in signaling by growth factor receptors. 脂筏和小泡在生长因子受体信号传导中的作用。

Open Biochemistry Journal Pub Date : 2007-01-01 Epub Date: 2007-09-13 DOI: 10.2174/1874091X00701010012

Angela de Laurentiis, Lorna Donovan, Alexandre Arcaro

引用次数: 74

Increased endogenous nitric oxide release by iron chelation and purinergic activation in the rat carotid body. 通过铁螯合和嘌呤能激活增加大鼠颈动脉体内源性一氧化氮释放。

Open Biochemistry Journal Pub Date : 2007-01-01 Epub Date: 2007-06-15 DOI: 10.2174/1874091X00701010001

Man-Lung Fung, Meifang Li, Sukhamay Lahiri

{"title":"Increased endogenous nitric oxide release by iron chelation and purinergic activation in the rat carotid body.","authors":"Man-Lung Fung, Meifang Li, Sukhamay Lahiri","doi":"10.2174/1874091X00701010001","DOIUrl":"https://doi.org/10.2174/1874091X00701010001","url":null,"abstract":"We examined the hypothesis that hypoxic chemotransduction with stabilization of HIF-1 and activation of purinoceptors stimulate the endogenous NO production in the rat carotid body. The effects of blockade of purinoceptors with suramin, or blockade of HIF-1alpha hydroxylation by suppressing prolyl hydroxylase (PAH) activity on the endogenous NO release measured electrochemically by microsensor inserted into the isolated carotid body superfused with bicarbonate-buffer were examined. Suramin did not change the resting NO level under normoxic conditions but it significantly decreased the hypoxia-induced NO elevation in a dose-dependent manner. Suramin (100muM) blocked the NO response to acute hypoxia by 53%. Intracellular iron chelator, ciclopirox olamine (CPX) significantly increased the resting NO release close to the hypoxic level, which was reversed by FeSO(4) or blocked by L-NMMA. Also, PAH inhibition with dimethy-loxalylglycine (DMOG) moderately increased the resting NO release. In the presence of CPX and DMOG the resting NO release was increased to the hypoxic level. Collectively, results suggest that iron chelation and purinoceptor stimulation play a role in the hypoxic chemotransduction for an increase in the endogenous NO production in the rat carotid body.","PeriodicalId":38958,"journal":{"name":"Open Biochemistry Journal","volume":" ","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570544/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27816319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5