Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology最新文献

筛选
英文 中文
Redistribution of connexin45 in gap junctions of connexin43-deficient hearts. 连接蛋白 45 在连接蛋白 43 缺失的心脏间隙连接中的重新分布。
IF 10.8
Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology Pub Date : 2002-03-01 DOI: 10.1016/s0008-6363(01)00522-3
Carolyn M Johnson, Evelyn M Kanter, Karen G Green, James G Laing, Tetsuo Betsuyaku, Eric C Beyer, Thomas H Steinberg, Jeffrey E Saffitz, Kathryn A Yamada
{"title":"Redistribution of connexin45 in gap junctions of connexin43-deficient hearts.","authors":"Carolyn M Johnson, Evelyn M Kanter, Karen G Green, James G Laing, Tetsuo Betsuyaku, Eric C Beyer, Thomas H Steinberg, Jeffrey E Saffitz, Kathryn A Yamada","doi":"10.1016/s0008-6363(01)00522-3","DOIUrl":"10.1016/s0008-6363(01)00522-3","url":null,"abstract":"<p><strong>Objective: </strong>Adult ventricular myocytes express two gap junction channel proteins: connexin43 (Cx43) and connexin45 (Cx45). Cx43-deficient mice exhibit slow ventricular epicardial conduction, suggesting that Cx43 plays an important role in intercellular coupling in the ventricle. Cx45 is much less abundant than Cx43 in working ventricular myocytes. Its role in ventricular conduction has not been defined, nor is it known whether expression or distribution of Cx45 is altered in Cx43-deficient mice. The present study was undertaken to determine (1) whether expression of Cx45 is upregulated and (2) whether gap junction structure and distribution are altered in Cx43-deficient mice.</p><p><strong>Methods: </strong>Ventricular tissue from neonatal Cx43(+/+), Cx43(+/-) and Cx43(-/-) and adult Cx43(+/+) and Cx43(+/-) mice was analyzed by immunoblotting and confocal immunofluorescence microscopy.</p><p><strong>Results: </strong>Total Cx45 protein abundance measured by immunoblotting was not different in Cx43-deficient or null hearts compared to wild-type control hearts. However, the amount and distribution of Cx45 immunoreactive signal measured by quantitative confocal analysis were markedly reduced in both Cx43(+/-) and Cx43(-/-) hearts.</p><p><strong>Conclusion: </strong>Although the total content of Cx45 is not upregulated in Cx43-deficient hearts, the localization of Cx45 to cardiac gap junctions depends on the expression level of Cx43 and is dramatically altered in mice that express no Cx43.</p>","PeriodicalId":38614,"journal":{"name":"Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology","volume":"38 1","pages":"921-35"},"PeriodicalIF":10.8,"publicationDate":"2002-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/s0008-6363(01)00522-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"55395565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 49
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信