Multidimensional Flow Cytometry Techniques for Novel Highly Informative Assays最新文献

Multidimensional Flow Cytometry for Testing Blood-Handling Medical Devices 多维流式细胞术检测血液处理医疗设备

Multidimensional Flow Cytometry Techniques for Novel Highly Informative Assays Pub Date : 2018-06-27 DOI: 10.5772/INTECHOPEN.76437

I. L. Pieper, Gemma Radley, C. Thornton

{"title":"Multidimensional Flow Cytometry for Testing Blood-Handling Medical Devices","authors":"I. L. Pieper, Gemma Radley, C. Thornton","doi":"10.5772/INTECHOPEN.76437","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.76437","url":null,"abstract":"Blood handling devices such as left ventricular assist devices and total artificial hearts offer life-saving treatments for patients suffering from severe heart failure. Current devices have clinically proven that heart assist pumps are a safe and effective therapy, and indeed in many cases they are the only available method of treatment. However, current devices cause side effects including stroke, bleeding, infection, and thrombosis, preventing the technology from reaching its full potential. If the side effects could be reduced, then more patients could benefit from these devices. The complications are related to damage to blood cells and proteins as a result of contact with foreign materials and mechanical stress. There is a need for better devices with minimal blood impact to enable more patients to be safely treated; better tools, especially flow cytometry, could support the device development life cycle. In this chapter we review the clinical, in vivo, and in vitro flow cytometry data available for ventricular assist devices, conduct a gap analysis, and identify areas of future possibilities for device developers to establish new flow cytometry-based methodologies.","PeriodicalId":383555,"journal":{"name":"Multidimensional Flow Cytometry Techniques for Novel Highly Informative Assays","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115859348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight into the Leukemia Microenvironment and Cell-cell Interactions Using Flow Cytometry 利用流式细胞术深入了解白血病微环境和细胞间相互作用

Multidimensional Flow Cytometry Techniques for Novel Highly Informative Assays Pub Date : 2018-06-27 DOI: 10.5772/INTECHOPEN.76481

K. Piwocka, Paulina Podszywalow-Bartnicka, Julian Swatler, Marta D Kolba, Agata Kominek, E. Kozłowska

{"title":"Insight into the Leukemia Microenvironment and Cell-cell Interactions Using Flow Cytometry","authors":"K. Piwocka, Paulina Podszywalow-Bartnicka, Julian Swatler, Marta D Kolba, Agata Kominek, E. Kozłowska","doi":"10.5772/INTECHOPEN.76481","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.76481","url":null,"abstract":"Cancer cells, including leukemia cells, reside in a complex microenvironment, which influences biology and activity of the cells. The protective role of bone marrow stromal cells is already commonly recognized. Remodeling of stroma cell functions by leukemia cells is also well documented. In this respect, different routes of interactions were defined, such as direct cell-cell interactions or indirect cross talk, by release of soluble factors or vesicular particles containing proteins, RNAs and other molecules. Since intercellular communication seems to play a role in various biological processes, it might be impor - tant to conduct studies in co-culture systems, which at least mimic partially more physi ological conditions, and enables this intercellular exchange to occur. Thus, it is crucial to improve analytical methods of investigation of co-cultured cells, to study their interac tions and so to understand biology of leukemia in order to understand molecular mecha - nisms and offer novel therapeutic strategies. The present chapter outlines the importance of modern, multiparameter flow cytometry methods, which allow to analyze interactions between different types of cells within the leukemia microenvironment. Importantly, the proposed experimental setups can be easily transformed to study different cell types and different biological systems.","PeriodicalId":383555,"journal":{"name":"Multidimensional Flow Cytometry Techniques for Novel Highly Informative Assays","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131702259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

High Throughput Screen for Inhibitors of Rac1 GTPase by Flow Cytometry 流式细胞术高通量筛选Rac1 GTPase抑制剂

Multidimensional Flow Cytometry Techniques for Novel Highly Informative Assays Pub Date : 2017-12-20 DOI: 10.5772/INTECHOPEN.71074

C. Bardelle, V. Sauzeau, Mark B. Carter, Zhaoping Liu, G. Loirand, David C Murray

引用次数: 1

Accurate and High Sensitivity Identification of PNH Clones by Flow Cytometry 流式细胞术对PNH克隆的准确、高灵敏度鉴定

Multidimensional Flow Cytometry Techniques for Novel Highly Informative Assays Pub Date : 2017-12-20 DOI: 10.5772/INTECHOPEN.71286

I. Marinov, A. Illingworth, D. Sutherland

{"title":"Accurate and High Sensitivity Identification of PNH Clones by Flow Cytometry","authors":"I. Marinov, A. Illingworth, D. Sutherland","doi":"10.5772/INTECHOPEN.71286","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.71286","url":null,"abstract":"Flow cytometry performs a key role in the diagnosis of paroxysmal nocturnal hemoglo- binuria (PNH). Careful selection and validation of antibody conjugates have allowed the development of reagent cocktails suitable for the high sensitivity detection of PNH red blood cells (RBCs) and white blood cells (WBCs) in PNH and related diseases such as aplastic anemia (AA) and some subsets of myelodysplastic syndromes (MDS). A CD235a- FITC/CD59-PE assay was developed capable of detecting Type III PNH RBCs at a limit of quantification (LOQ) of 0.01% or better. While separate 4-color Fluorescent Aerolysin (FLAER), CD24, CD15 and CD45-based neutrophil and FLAER, CD14, CD64 and CD45-based monocyte assays were developed to detect PNH WBC phenotypes, 5-, 6- and 7-color assays have subsequently been developed for more modern cytometers equipped with five or more fluorescence detectors. For instrumentation with five detectors, a single tube 5-color FLAER, CD157, CD15, CD64 and CD45-based assay to simultaneously detect PNH neutrophils and monocytes has been developed. For instruments with six or more detectors and multiple lasers, a variety of 5-, 6- and 7-color assays have been developed using combinations of FLAER, CD24, CD14 and CD157. All WBC assays have a limit of quantification (LOQ) of 0.1% or better. Using these standardized approaches, results have demonstrated good intra- and inter-laboratory performance characteristics even in laboratories with little prior experience performing PNH testing.","PeriodicalId":383555,"journal":{"name":"Multidimensional Flow Cytometry Techniques for Novel Highly Informative Assays","volume":"479 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122742796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient Interpretation of Multiparametric Data Using Principal Component Analysis as an Example of Quality Assessment of Microalgae 基于主成分分析的多参数数据高效解释——以微藻质量评价为例

Multidimensional Flow Cytometry Techniques for Novel Highly Informative Assays Pub Date : 2017-12-20 DOI: 10.5772/INTECHOPEN.71460

Toshiyuki Takahashi

{"title":"Efficient Interpretation of Multiparametric Data Using Principal Component Analysis as an Example of Quality Assessment of Microalgae","authors":"Toshiyuki Takahashi","doi":"10.5772/INTECHOPEN.71460","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.71460","url":null,"abstract":"Multiparametric flow cytometry (FCM) realizes high-throughput measurement, but mul- tiparametric data make it difficult to interpret the complicated information. To present clear patterning graphs from FCM data, one must grasp the essence of the data. This study estimated the usefulness of principal component analysis (PCA), which reduces multidimensional information to arbitrary one-dimensional information. Recently, microalgae have attracted the attention of pharmaceutical, cosmetic, and food companies. Taking alga Chlorella as an example, this chapter presents the usefulness of PCA for the evaluation of algal quality using FCM. To evaluate the algal status effectively, Chlorella (control), heated algae, and metallic-treatment algae were prepared and quantified using FCM. FCM data were subjected to PCA analysis. To interpret correlativity among parameters, FCM data are generally expressed as histograms and scatter or contour plots. An operator using multiple parameters has difficulty finding high correlativity among parameters and pre-senting an effective graph. The PCA method produced new comprehensive axes with different inclination factors among parameters. Scatter plots using new axes showed patterns treatment dependently with different vectors. Results show that the PCA method can extract information of test objects from data and that it can contribute to effective interpretation of cell characteristics, even if data include multiparameters from FCM.","PeriodicalId":383555,"journal":{"name":"Multidimensional Flow Cytometry Techniques for Novel Highly Informative Assays","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115056984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3