{"title":"Carrot slice test: A reliable method for evaluating the tumorigenicity of Pseudomonas savastanoi pv. nerii","authors":"Fodor Attila, Juhasz Áron, Vitári Viktória, Anita Karacs-Végh","doi":"10.17352/ojb.000026","DOIUrl":"https://doi.org/10.17352/ojb.000026","url":null,"abstract":"Pathogenicity studies on oleander plants take a long time and require significant costs to confirm the identification of isolates and to verify their pathogenicity. In this study, the carrot slice test was used as a rapid method for Pseudomonas savastanoi pv. nerii. The test is suitable for the investigation of tumorigenicity of 25 Pseudomonas savastanoi pv. nerii isolates from different parts of Hungary. On carrot slices, the first characteristic knots were observed 7 days after inoculation and were fully developed 16 days after inoculation. The one-year oleander plants used to confirm the pathogenicity of the bacteria were inoculated with a bacterial suspension to confirm the reliability of the carrot slice test. Characteristic symptoms of knots were observed on all oleander plants 46 days after inoculation. The results showed that the carrot slice method is a straightforward, rapid, and reliable method for testing and confirming tumor formation of Pseudomonas savastanoi pv. nerii without plants.","PeriodicalId":378260,"journal":{"name":"Open Journal of Bacteriology","volume":" 26","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139628231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Extraction of silica from natural deposits for the production of silicon in photovoltaic applications","authors":"Zeboudj Asmaa, Hamzaoui Saad","doi":"10.17352/ojb.000024","DOIUrl":"https://doi.org/10.17352/ojb.000024","url":null,"abstract":"The Silica, primarily sourced from silicon dioxide (SiO2) is a common chemical compound abundant in nature, existing in various forms such as quartz, sand, glass, and diverse minerals. It finds extensive use across multiple industries, contributing significantly to glassmaking, ceramics, abrasives, refractory materials, and serving as a crucial component in semiconductor production for electronic chips. This work provides a comprehensive review of the silica elaboration process aimed at obtaining silicon. The initial stage involves the preparation of raw materials, utilizing Algerian sand and diatomite, which undergo meticulous chemical treatment to eliminate unwanted impurities. This method comprises distinct steps, known as the purification process, wherein the sand or diatomite shell is assessed through dissolution in a caustic alkaline solution. This approach indicates the potential to generate high-quality silica from diatomite using an aqueous chemical process. This paper explores the prospect of sand or diatomaceous earth as a novel source of premium-grade silica. It discusses the effects of hydrochloric acid as a solvent for purification. This study contrasts treatment methods before and after acid leaching, focusing on samples with larger particle sizes (ranging from 53 to 300 microns), shorter refining durations (1 to 6 hours), and relatively higher temperatures (30 °C to 70 °C). The findings reveal that the highest purity of silica was attained through HCl treatment (25%).","PeriodicalId":378260,"journal":{"name":"Open Journal of Bacteriology","volume":"84 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139008030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Systematic review–how do we identify urinary tract infections today?","authors":"Čeprnja Marina, Oros Damir, Janeš Andrea, Žučko Jurica, Barišić Karmela, Škrlin Jasenka, Starčević Antonio","doi":"10.17352/ojb.000023","DOIUrl":"https://doi.org/10.17352/ojb.000023","url":null,"abstract":"Standard urine culture is still considered a gold standard in the identification of Urinary Tract Infections (UTIs), but is time-consuming and in approximately 20% of patients with UTI symptoms produces false-negative results. Medical and scientific communities are in search of a faster, more accurate, yet affordable method with high clinical utility. As a supplement to standard culture in routine practice Urine Flow Cytometer (UFC) screening method is used, in order to detect negative urine culture samples. This allows for shortening issuing time for sterile urine culture reports and the cost of the analysis itself. In addition, urine dipstick tests and microscopic examinations of urine sediment can also be performed in biochemical laboratories but are usually preceded by urine culture. Nowadays, advanced methods such as proteomics and genomics are used to identify pathogens causing UTIs but are still used mainly for scientific purposes and rarely in clinical practice. From genomic methods PCR, 16S rRNA gene sequencing, and metagenome sequencing are being researched. PCR is great for targeted diagnostics, 16S RNA gene amplification can determine bacterial genera and their abundance, but is not good for in-depth species analysis, while metagenomics is the most comprehensive and unbiased method. The proteomics field also offers several methods for microbial identification, with MS as the leading one. Clinical applications of MS platforms usually imply MALDI-TOF MS analyzers which produce a characteristic spectrum called peptide mass fingerprint or more present for scientific purposes LC-MS/MS-based peptide sequencing.","PeriodicalId":378260,"journal":{"name":"Open Journal of Bacteriology","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130653887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antimicrobial resistance: The next probable pandemic","authors":"U. Krishnapriya","doi":"10.17352/ojb.000022","DOIUrl":"https://doi.org/10.17352/ojb.000022","url":null,"abstract":"Even as the world continues to grieve for those lost to the SARS-CoV-2 pandemic, another pandemic is brewing that has the potential to kill hundreds of millions of people in a century or less. In the far future, patients’ lives are being cut short in hospital wards by the stealthy but widespread epidemic of antimicrobial resistance. Unfortunately, the global health community is now confronting the silently growing pandemic that could threaten some of the most significant advancements in contemporary medicine. Considering that current medical professionals will eventually pass the torch to their students, who will hopefully lead to further improvements in antimicrobial resistance and antibiotic usage, medical students have the opportunity to contribute to a long-term solution to this problem. Future medical professionals, then, need to be better equipped to make prudent and economical use of antimicrobials.","PeriodicalId":378260,"journal":{"name":"Open Journal of Bacteriology","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129111767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Edem Ekom Ndifreke, Umo Anthony Nathaniel, Akinjogunla Olajide Joseph, Elahmar Adel AE, Arome David
{"title":"Detection rate of pulmonary tuberculosis by direct and concentrated microscopy techniques with Lowenstein-Jensen Culture as standard: South-South Nigeria Scenario","authors":"Edem Ekom Ndifreke, Umo Anthony Nathaniel, Akinjogunla Olajide Joseph, Elahmar Adel AE, Arome David","doi":"10.17352/ojb.000021","DOIUrl":"https://doi.org/10.17352/ojb.000021","url":null,"abstract":"Objective: This study seeks to identify a more sensitive smear microscopy method for the detection of Mycobacterium tuberculosis in low-resource centers. Materials and methods: One hundred and forty sputum specimens were collected and direct smears were prepared as the specimen was submitted. The specimens were afterward digested/decontaminated using the modified Petroff’s method. Both smear methods were stained by the Ziehl-Neelsen technique and examined. All specimens (direct and concentrated) were cultured on a Lowenstein-Jensen medium and results were considered the gold standard to calculate sensitivity. Results: Out of 140 specimens, 27 (19%) and 34 (24%) were positive by direct and concentrated methods respectively. The number of culture positives by the direct and concentrated method was 26 (19%) and 31(22%) respectively. The sensitivity of direct and concentrated methods was 92.3% and 100% respectively. The negative culture and contamination rate was minimum with the concentrated method. The diagnostic accuracy of direct and concentrated methods was 88.6% and 97.1%. Conclusion: In summary, results showed concentrated techniques were able to detect more true positive Mycobacterium tuberculosis than the direct smear method. Therefore, the study region with AFB smear microscopy can utilize the concentrated smear method for AFB smear microscopy to improve the case-finding strategy.","PeriodicalId":378260,"journal":{"name":"Open Journal of Bacteriology","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121126729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hericium erinaceus powder inhibits the growth of Porphyromonas gingivalis","authors":"I. Hirono, Kawai Junya, Mori Koichiro","doi":"10.17352/ojb.000020","DOIUrl":"https://doi.org/10.17352/ojb.000020","url":null,"abstract":"Identifying the alterations occurred in the bacteriology of the dermic layer of the abdominal wall in patients who underwent a colostomy, through the qualitative and quantitative determination of the pericolostomic colonization.","PeriodicalId":378260,"journal":{"name":"Open Journal of Bacteriology","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127011032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mouad Fatima Zahra, S. Elmoussaoui, K. Elfakiri, N. Rada, G. Draiss, N. Soraa, M. Bouskraoui
{"title":"Purulent pleurisy in children","authors":"Mouad Fatima Zahra, S. Elmoussaoui, K. Elfakiri, N. Rada, G. Draiss, N. Soraa, M. Bouskraoui","doi":"10.17352/ojb.000016","DOIUrl":"https://doi.org/10.17352/ojb.000016","url":null,"abstract":"The incidence of purulent pleurisy is on the rise in several series in the literature. It is a significant cause of morbidity in pediatrics. The main objective of our study is to analyze the epidemiological characteristics and the prognosis of purulent pleurisy of the child.","PeriodicalId":378260,"journal":{"name":"Open Journal of Bacteriology","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125216941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Open Journal of Bacteriology","authors":"A. Tiburcio, H. Ruiz, P. Reyes-López","doi":"10.17352/OJB.000010","DOIUrl":"https://doi.org/10.17352/OJB.000010","url":null,"abstract":"","PeriodicalId":378260,"journal":{"name":"Open Journal of Bacteriology","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131432646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Leonard Katunzi, G. Bwire, James Kalabashanga, Lilian Nkinda, Akili Mawazo, K. Mwambete
{"title":"Diagnostic accuracy of dipsticks test among clinically suspected urinary tract infected patients at National Hospital, Tanzania","authors":"Leonard Katunzi, G. Bwire, James Kalabashanga, Lilian Nkinda, Akili Mawazo, K. Mwambete","doi":"10.17352/OJB.000009","DOIUrl":"https://doi.org/10.17352/OJB.000009","url":null,"abstract":"Background: Dipstick test is a rapid test for diagnosis of urinary track infections (UTIs). Rapid \u0000test should be accurate so that does not give a lot of false results that may subject patients to wrong \u0000treatments especially when used alone. Therefore, this study was set to determine the diagnostic \u0000performance of dipsticks tests by comparing with a standard urine culture","PeriodicalId":378260,"journal":{"name":"Open Journal of Bacteriology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115744616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Understanding about glanders among students at Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan","authors":"Muhammad Hamad Saif, M. Qadir","doi":"10.17352/ojb.000008","DOIUrl":"https://doi.org/10.17352/ojb.000008","url":null,"abstract":"Objective of present study was to understand the knowledge of students about infection, causes and cure of glanders. Glanders is an infection in humans and in animals. It is a bacterial disease caused by Burkholderia mallei. Glanders is mainly a disease which affect on horses, and some time cats, rabbits, humans or other animals. People are infected by direct contact with infected animals. The peoples have been suffered by glanders and it affect on their nasal discharge, saliva and in urine. Important information was collected by many questions e.g. is glanders a bacterial or viral, genetic or metabolic? 200 students participated in this project.","PeriodicalId":378260,"journal":{"name":"Open Journal of Bacteriology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120949512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}