Biophysics, Biology and Biophotonics IV: the Crossroads最新文献

Biophysics, Biology and Biophotonics IV: the Crossroads Pub Date : 2019-05-16 DOI: 10.1117/12.2531657

引用次数: 0

Functional imaging with light and sound including microcirculation (Conference Presentation) 包括微循环在内的光声功能成像(会议报告)

Biophysics, Biology and Biophotonics IV: the Crossroads Pub Date : 2019-03-13 DOI: 10.1117/12.2513413

M. Leahy

引用次数: 0

Spectral based tissue classification and ultrastructural characterization using ISOCT (Conference Presentation) 基于光谱的组织分类和使用ISOCT的超微结构表征(会议报告)

Biophysics, Biology and Biophotonics IV: the Crossroads Pub Date : 2019-03-04 DOI: 10.1117/12.2513825

A. Eid, J. A. Winkelmann, G. Spicer, L. Almassalha, V. Backman

{"title":"Spectral based tissue classification and ultrastructural characterization using ISOCT (Conference Presentation)","authors":"A. Eid, J. A. Winkelmann, G. Spicer, L. Almassalha, V. Backman","doi":"10.1117/12.2513825","DOIUrl":"https://doi.org/10.1117/12.2513825","url":null,"abstract":"Alterations to nanoscale structures, lymphatics, and microvasculature are early hallmarks of neoplasia as well as a variety of other diseases. Unfortunately, nanoscale alterations and microvasculature function, such as oxygen saturation, cannot be probed by histology. Furthermore, properly evaluating lymphatic and microvasculature organization can be challenging with histological slices. Optical Coherence Tomography (OCT) offers a promising noninvasive solution to evaluating these biomarkers in 3D in vivo. \u0000\u0000OCT has shown the ability to provide 3D maps of vasculature with flow rate and blood oxygenation, as well as, lymphatic organization with a resolution on the order of 1-10 microns. Our group has established Inverse Spectroscopic OCT (ISOCT), which measures nanoscale mass density tissue fluctuations and can distinguish between histologically normal cancerous and noncancerous tissue. However, the most influential underlying assumption that allows the distinction between subdiffractional structural alterations in tissue is that the region of interest (ROI) includes a homogenous tissue type with similar scattering and absorption properties. Therefore, the highly absorbing blood and low scattering lymphatics must be excluded from analysis. \u0000\u0000Traditional OCT techniques to isolate vasculature and its spectra require timely repetitive scanning protocols, and the commonly utilized near infrared operating bandwidths require vessel-like filters to locate lymphatics. Herein we show how vasculature location and spectra can be extracted with a single visible OCT scan. Additionally, we demonstrate the high image contrast from visible OCT allows lymphatic location to be well defined. Finally, we show ultrastructural metrics fall within physiologically reasonable ranges after excluding vasculature and lymphatics from the ROI.","PeriodicalId":373171,"journal":{"name":"Biophysics, Biology and Biophotonics IV: the Crossroads","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115064262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elastic light scattering spectroscopy in living human retina (Conference Presentation) 活体视网膜弹性光散射光谱研究(会议报告)

Biophysics, Biology and Biophotonics IV: the Crossroads Pub Date : 2019-03-04 DOI: 10.1117/12.2513716

Ji Yi

引用次数: 0

Comparing quantitative phase derived cellular mechanical parameters with atomic force microscopy measurements (Conference Presentation) 比较定量相导出的细胞力学参数与原子力显微镜测量(会议报告)

Biophysics, Biology and Biophotonics IV: the Crossroads Pub Date : 2019-03-04 DOI: 10.1117/12.2510292

W. Eldridge, Silvia Ceballos, H. Park, A. Wax

{"title":"Comparing quantitative phase derived cellular mechanical parameters with atomic force microscopy measurements (Conference Presentation)","authors":"W. Eldridge, Silvia Ceballos, H. Park, A. Wax","doi":"10.1117/12.2510292","DOIUrl":"https://doi.org/10.1117/12.2510292","url":null,"abstract":"Cellular viscoelasticity is a biomarker for cancer type and toxin exposure. Current standard methods for probing cellular stiffness are slow, laborious, and utilize complex or indirect detection. These limitations prevent effective study of changes to viscoelasticity over time as well as longitudinal study of single cells. To enable direct and non-contact measurement of stiffness, we developed a quantitative phase imaging (QPI) based method to directly measure mechanical displacement in living cells in response to static loading. We calculated mechanical parameters, including shear stiffness, to discriminate between different cancer types and cell types that were exposed to varied levels of environmental and pharmacological toxins. We also demonstrated a correlation between our shear stiffness parameter and disorder strength, a measure of cellular refractive index homogeneity acquired via a single QPI image, showing the feasibility of high-throughput, nondestructive mechanical measurements. \u0000\u0000Now, we compare our methods to atomic force microscopy (AFM), the gold standard for measuring cellular viscoelastic characteristics. We evaluate multiple breast cancer cell lines that are dosed with varying concentrations of cytochalasin B, an actin network-disrupting toxin. Each group is characterized by a commercial AFM to measure Young’s modulus and indentation stiffness. The same groups are analyzed using our QPI system to simultaneously measure shear stiffness and disorder strength. Relationships between all four measurements are analyzed to determine the correlation between the QPI derived parameters and those found using the commercial AFM, and to explore the feasibility of using QPI as a high-throughput alternative to AFM for measurements of cellular viscoelasticity.","PeriodicalId":373171,"journal":{"name":"Biophysics, Biology and Biophotonics IV: the Crossroads","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132985603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Optical imaging of the cochlea (Conference Presentation) 耳蜗光学成像(会议报告)

Biophysics, Biology and Biophotonics IV: the Crossroads Pub Date : 2019-03-04 DOI: 10.1117/12.2513591

D. Psaltis, M. Romito

引用次数: 0

Color spatial light interference microscopy (cSLIM) for quantitative histopathology of stained tissues (Conference Presentation) 彩色空间光干涉显微镜(cSLIM)用于染色组织的定量组织病理学(会议报告)

Biophysics, Biology and Biophotonics IV: the Crossroads Pub Date : 2019-03-04 DOI: 10.1117/12.2513805

G. Popescu

引用次数: 0