发布求助
文献互助 智能选刊 最新文献

Molecular Soil Biology最新文献

筛选
英文 中文
Purification and Optimization of Prokaryotic Expression of CSN5B Protein of Arabidopsis thaliana 拟南芥CSN5B蛋白的纯化及原核表达优化
Molecular Soil Biology Pub Date : 2020-02-26 DOI: 10.5376/MSB.2020.11.0002
Xueliang Niu, Lili Wang, Shenkui Liu, Yuanyuan Bu
{"title":"Purification and Optimization of Prokaryotic Expression of CSN5B Protein of Arabidopsis thaliana","authors":"Xueliang Niu, Lili Wang, Shenkui Liu, Yuanyuan Bu","doi":"10.5376/MSB.2020.11.0002","DOIUrl":"https://doi.org/10.5376/MSB.2020.11.0002","url":null,"abstract":"CSN5B is a subunit of the COP9 signalosome (CSN) and plays physiological functions in the form of monomer or complex in plants. The CSN5B of Arabidopsis thaliana was cloned by RT-PCR technology and constructed into the prokaryotic expression vector pET-32a (+), which was introduced into E.coli BL21 host bacteria. Isopropylβ-D-1-thiogalactopyranoside (IPTG) was used to induce expression, and the cultural temperature and time was optimized. The sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was employed to detect the induced recombination protein, and the protein purification was conducted. It was shown that the recombinant CSN5B introduced into E. coli could be induced to express, and the induction was relatively effective in the presence of 1mmol/L of IPTG at 30℃, 4h induction effect is better. The molecular weight of the induced recombinant protein was basically consistent with the theoretical molecular weight. This study provided a theoretical basis for in-depth further exploration of the functions of this gene in the future.","PeriodicalId":365098,"journal":{"name":"Molecular Soil Biology","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128281047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Statistics and Analyses System of Microbial Community Level Physiological Profiles (CLPP) Based on Excel 基于Excel的微生物群落水平生理剖面统计分析系统
Molecular Soil Biology Pub Date : 2019-08-30 DOI: 10.5376/msb.2019.10.0001
Lin Li, Yanjie Zhu, Songyan Tian, Qian Du, Shenkui Liu
{"title":"A Statistics and Analyses System of Microbial Community Level Physiological Profiles (CLPP) Based on Excel","authors":"Lin Li, Yanjie Zhu, Songyan Tian, Qian Du, Shenkui Liu","doi":"10.5376/msb.2019.10.0001","DOIUrl":"https://doi.org/10.5376/msb.2019.10.0001","url":null,"abstract":"Microbial community level physiological profiles (CLPP) assessed using Biolog MicroPlates has effectively demonstrated the metabolic activities of multiple microbes. Due to a large volume of data, and complicated calculation process, the conventional low efficiency calculations delay the study. To empower researchers to quickly tabulate, compute, analyze and present data, we introduced A Biolog EcoPlate data statistics and analyses system (A-BEP-DSAS). It is implemented with Biolog EcoPlate data tested by an ELISA microplate reader (Molecular Devices Versamax). The data input-output process and visual calculation flow are performed using Excel table linked with built-in VBA programming language. We tested A-BEP-DSAS by data from a soil sample, and rapidly and accurately obtained the average well color development (AWCD) data, carbon source utilization, six common diversity indices and other calculation results. A-BEP-DSAS is an automated program based on Microsoft Excel VBA, with the advantages of easy operation, rapid analysis, process visualization, and also allows the user to choose the data source and optimum time for analysis.","PeriodicalId":365098,"journal":{"name":"Molecular Soil Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129386527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Expression and Purification of Arabidopsis CASPL1D2 Protein 拟南芥CASPL1D2蛋白的表达与纯化
Molecular Soil Biology Pub Date : 1900-01-01 DOI: 10.5376/msb.2023.14.0002
Yujie Qu, Shenkui Liu, Yuanyuan Bu
{"title":"Expression and Purification of Arabidopsis CASPL1D2 Protein","authors":"Yujie Qu, Shenkui Liu, Yuanyuan Bu","doi":"10.5376/msb.2023.14.0002","DOIUrl":"https://doi.org/10.5376/msb.2023.14.0002","url":null,"abstract":"","PeriodicalId":365098,"journal":{"name":"Molecular Soil Biology","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126280237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Research Progress of Urea Transporter DUR3 in Plants and Fungi 植物和真菌尿素转运蛋白DUR3的研究进展
Molecular Soil Biology Pub Date : 1900-01-01 DOI: 10.5376/msb.2022.13.0002
Xueying Li, Dan Su, Shenkui Liu, Yuanyuan Bu
{"title":"Research Progress of Urea Transporter DUR3 in Plants and Fungi","authors":"Xueying Li, Dan Su, Shenkui Liu, Yuanyuan Bu","doi":"10.5376/msb.2022.13.0002","DOIUrl":"https://doi.org/10.5376/msb.2022.13.0002","url":null,"abstract":"","PeriodicalId":365098,"journal":{"name":"Molecular Soil Biology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114706550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Research Progress of Plant Rab Proteins 植物Rab蛋白的研究进展
Molecular Soil Biology Pub Date : 1900-01-01 DOI: 10.5376/msb.2023.14.0001
Rongrong Zhang, Menglu Cai, Shenkui Liu, Yuanyuan Bu
{"title":"Research Progress of Plant Rab Proteins","authors":"Rongrong Zhang, Menglu Cai, Shenkui Liu, Yuanyuan Bu","doi":"10.5376/msb.2023.14.0001","DOIUrl":"https://doi.org/10.5376/msb.2023.14.0001","url":null,"abstract":"","PeriodicalId":365098,"journal":{"name":"Molecular Soil Biology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129737316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信