Clinical and Translational Neurophotonics 2019最新文献

{"title":"Front Matter: Volume 10864","authors":"","doi":"10.1117/12.2531324","DOIUrl":"https://doi.org/10.1117/12.2531324","url":null,"abstract":"","PeriodicalId":364545,"journal":{"name":"Clinical and Translational Neurophotonics 2019","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126643071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imaging of cerebral hemodynamic and light scattering responses to somatosensory electrical stimulation using a digital RGB color camera (Conference Presentation)","authors":"I. Nishidate, Takayuki Tsubonuma, M. Inaba, S. Kawauchi, Shunichi Sato, M. Sato, Y. Kokubo","doi":"10.1117/12.2509821","DOIUrl":"https://doi.org/10.1117/12.2509821","url":null,"abstract":"We investigated a rapid imaging method to monitor the spatial distribution of total hemoglobin concentration (CHbT), the tissue oxygen saturation (StO2), and the scattering power b in the expression of musp=a(lambda)^-b as the scattering parameters in cerebral cortex using a digital red-green-blue camera. In the method, Monte Carlo simulation (MCS) for light transport in brain tissue is used to specify a relation among the RGB-values and the concentration of oxygenated hemoglobin (CHbO), that of deoxygenated hemoglobin (CHbR), and the scattering power b. In the present study, we performed sequential recordings of RGB images of in vivo exposed brain of rats before, during, and after hindlimb electrical stimulation. The remarkable increases in CHbO, CHbT, and StO2 were induced by hindlimb electrical stimulation whereas significant decreases in the scattering power b and CHbR were observed after the onset of stimulation. It has been reported that cerebral blood flow (CBF) and blood oxygen level-dependent (BOLD) signal responses show better correlation with post-synaptic local field potentials than with spiking activity. Positive CBF and BOLD responses during stimulation are associated with an increase in neuronal activity and decrease in deoxyhemoglobin content. Therefore, the decrease in the scattering power b of somatosensory cortex after hindlimb electrical stimulation is indicative of slow post-synaptic potential change. The results in this study indicate potential of RGB camera-based imaging to evaluate both hemodynamics and synaptic activity in brain tissue.","PeriodicalId":364545,"journal":{"name":"Clinical and Translational Neurophotonics 2019","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131333182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Connecting theory to the signal dynamics of interferometric diffuse correlation spectroscopy (Conference Presentation)","authors":"Mitchell B. Robinson, S. Carp, D. Tamborini, D. Boas, M. Franceschini","doi":"10.1117/12.2510153","DOIUrl":"https://doi.org/10.1117/12.2510153","url":null,"abstract":"","PeriodicalId":364545,"journal":{"name":"Clinical and Translational Neurophotonics 2019","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131855142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering 3D living brain tumour equivalents as macroscopic test-systems for the development of optical imaging and sensing applications\u0000\u0000 (Conference Presentation)","authors":"A. Nadort, M. V. Zadeh, Sameera Iqbal, Mina Ghanimi Fard, D. Polikarpov, S. Sachdev, Lindsay M. Parker, Qian-Yan Yi, A. Davidson, N. Packer, E. Goldys, Anna E Guller","doi":"10.1117/12.2509582","DOIUrl":"https://doi.org/10.1117/12.2509582","url":null,"abstract":"","PeriodicalId":364545,"journal":{"name":"Clinical and Translational Neurophotonics 2019","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116723379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative cross-polarization OCT image analysis of ex vivo human brain tissues and its comparison with MRI and histological data (Conference Presentation)","authors":"E. Kiseleva, A. Moiseev, K. Yashin, S. Kuznetsov, I. Medyanik, G. Gelikonov, E. Zagaynova, N. Gladkova","doi":"10.1117/12.2511274","DOIUrl":"https://doi.org/10.1117/12.2511274","url":null,"abstract":"","PeriodicalId":364545,"journal":{"name":"Clinical and Translational Neurophotonics 2019","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121902934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reversible blood-brain barrier modulation enhances in vivo delivery of panitumumab-IRDye800 to high-grade glioma in cranial window model (Conference Presentation)","authors":"Quan Zhou, Christy Wilson, H. Vogel, N. Teraphongphom, R. Ertsey, Pauline Chu, G. Lu, N. S. Berg, Stan van Keulen, N. Nishio, G. Grant, E. Rosenthal","doi":"10.1117/12.2511180","DOIUrl":"https://doi.org/10.1117/12.2511180","url":null,"abstract":"Background: Pediatric High-grade gliomas (pHGGs) are the No.1 cause of cancer-related deaths in children with median survival of less than a year. pHGGs tend to be infiltrative and appear irregularly shaped with ill-defined borders difficult to be distinguished from surrounding normal brain tissue. As the extent of surgical resection predicts survival, precise tumor removal with more accurate margin delineation means better treatment outcome and less loss of vital functions. While EGFR is one of the most commonly amplified genes in pHGGs, its protein-level expression is not as well characterized as in adult HGGs. Previously, near-infrared (NIR) dye labeled epidermal growth factor receptor (EGFR) antibody has served as contrast agent in fluorescence-guided surgery of head and neck cancer. However, it must overcome the blood-brain barrier (BBB) for effective intratumoral delivery in the case of brain cancer. Therefore, the latest advancement in reversible BBB opening with tight junction protein modulation has the potential to enable the molecular targeted imaging guidance of pHGG resection.\u0000Aims: The current study aimed to improve intratumoral delivery of NIR fluorescent EGFR antibody via reversible BBB permeability enhancement with siRNA modulation of tight junction protein in an orthotopic xenograft animal model of high-grade glioma with EGFR overexpression. Furthermore, resected pHGGs were examined for EGFR expression in order to stratify patient subpopulation most likely to benefit from intraoperative molecular imaging strategy that targets EGFR.\u0000Methods: An orthotopic high-grade glioma xenograft model was established in 6-15 week old mice (n=3) by intracranial injection of 10^6 EGFR-overexpressing high-grade glioma cells (D270, 10ul) 3mm below the surface of brain. Subsequently, the exposed brain was covered with a glass plate secured to the skull with cyanoacrylate glue. siRNA was selected from those targeting conserved regions of the mouse claudin-5 cDNA sequence. 20μg of claudin-5 siRNA was injected intravenously via the tail vein in an in vivo-Jet-PEI solution (Polyplus Transfection) at a rate of 0.2 ml/sec 10 days post tumor implant. 0.1mL tetramethylrhodamine (250kDa) and various sized FITC-dextran (4.4-150kDa) solutions were injected intravenously to visualize blood vessels and assess extravasation distance through cranial window via 2-photo microscopy. Enhanced permeability of BBB was characterized by increase in KTrans on dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in the tumor region. Mean fluorescence intensity at 800nm was measured through cranial window with an in vivo NIR imager (Pearl Impulse, LI-COR Biosciences) 0-72 hours following tail vein injection of 200ug panitumumab-IRDye800 (pan800). Immunohistochemical analysis of EGFR expression was performed on surgically resected de novo primary pHGG tumors, from seven GBM and three anaplastic ependymoma patients respectively. \u0000Results: The siRNA has shown a rever","PeriodicalId":364545,"journal":{"name":"Clinical and Translational Neurophotonics 2019","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130228991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimulation and repair of peripheral nerves using a bioadhesive graft-antenna (Conference Presentation)","authors":"A. Lauto, Ashour Sliow, Zhi Ma, G. Garguilo, D. Mahns, D. Mawad, P. Breen, M. Stoodley, J. Houang, G. Tettamanzi, J. Morley, L. Longo, Rhiannon P. Kuchel","doi":"10.1117/12.2508255","DOIUrl":"https://doi.org/10.1117/12.2508255","url":null,"abstract":"","PeriodicalId":364545,"journal":{"name":"Clinical and Translational Neurophotonics 2019","volume":"248 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122497644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Portable high-density diffuse optical tomography for pediatric brain imaging in low-resource settings (Conference Presentation)","authors":"Andrew K. Fishell, E. Richter, C. Valdes, Margarita Torres, A. Eggebrecht, C. Smyser, A. Arbeláez, J. Culver","doi":"10.1117/12.2509859","DOIUrl":"https://doi.org/10.1117/12.2509859","url":null,"abstract":"","PeriodicalId":364545,"journal":{"name":"Clinical and Translational Neurophotonics 2019","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124980848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor angiogenesis imaging using an integrated photoacoustic microscopy with hybrid-resolution (Conference Presentation)","authors":"Yu-Hang Liu, Yu Xu, K. Chan, Gayathiri Magarajah, Aishwarya Bandla, N. Thakor","doi":"10.1117/12.2507360","DOIUrl":"https://doi.org/10.1117/12.2507360","url":null,"abstract":"","PeriodicalId":364545,"journal":{"name":"Clinical and Translational Neurophotonics 2019","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134023047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macrophage-mediated delivery of chemotherapeutics for photochemical internalization (Conference Presentation)","authors":"Stephanie Molina, H. Hirschberg, S. Madsen","doi":"10.1117/12.2506199","DOIUrl":"https://doi.org/10.1117/12.2506199","url":null,"abstract":"","PeriodicalId":364545,"journal":{"name":"Clinical and Translational Neurophotonics 2019","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114172882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}