Chinese Journal of Neonatology最新文献

筛选

英文中文

Dyspnea in a newborn infant born to mother suffering from novel coronavirus pneumonia 新型冠状病毒肺炎母亲所生新生儿呼吸困难

Chinese Journal of Neonatology Pub Date : 2020-03-15 DOI: 10.3760/CMA.J.ISSN.2096-2932.2020.02.002

Zhao Yiwei, Lin Zhen-lang, Ma Xujie, Jia Xiaole, Zhang Wei

引用次数: 1

Effect of calcium-sensitive receptor on intracellular Ca2+ concentration in pulmonary arterial smooth muscle cells of hypoxia-induced persistent pulmonary hypertension in mice 钙敏感受体对缺氧诱导的小鼠肺动脉平滑肌细胞内Ca2+浓度的影响

Chinese Journal of Neonatology Pub Date : 2018-01-15 DOI: 10.3760/CMA.J.ISSN.2096-2932.2018.01.015

He Li, Q. Gu, Meng-meng Wang, Weiwei Cao, Ke-Tao Ma

{"title":"Effect of calcium-sensitive receptor on intracellular Ca2+ concentration in pulmonary arterial smooth muscle cells of hypoxia-induced persistent pulmonary hypertension in mice","authors":"He Li, Q. Gu, Meng-meng Wang, Weiwei Cao, Ke-Tao Ma","doi":"10.3760/CMA.J.ISSN.2096-2932.2018.01.015","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.2096-2932.2018.01.015","url":null,"abstract":"Objective \u0000To study the changes of intracellular calcium ion concentration in pulmonary artery smooth muscle cells (PASMCs) of hypoxic-induced persistent pulmonary hypertension (PPH) induced by calcium-sensitive receptor (CaSR) in a newborn mouse model. \u0000 \u0000 \u0000Method \u0000Ninety-six newborn C57BL/6 mice were randomly divided into control group, PPH group, PPH+ agonist group and PPH+ inhibitor group, with 24 mice in each group. The PPH model was induced by 12% oxygen for 14 days. In the beginning, intraperitoneal injection of CaSR agonist (GdCl3) and CaSR inhibitor (NPS2390) were performed to mice in PPH+ agonist group and PPH+ inhibitor group respectively daily. After 14 days of modeling, pulmonary artery smooth muscle cells (PASMCs) of all four groups were cultured in vitro. Changes of Ca2+ fluorescence intensity in PASMCs of the four groups were detected by laser confocal microscope continuously. \u0000 \u0000 \u0000Result \u0000The ratio of pulmonary small vascular wall thickness to the vascular diameter and right ventricle/left ventricular thickness in PPH group were greater than those in the control group [(21.1%±1.8%) vs.(27.0%±0.9%), (0.62±0.22) vs.(0.83±0.45)], the differences were statistically significant (P 0.05). \u0000 \u0000 \u0000Conclusion \u0000CaSR may be involved in the occurrence and development of hypoxic-induced PPH in neonatal mice by affecting the intracellular Ca2+ concentration in pulmonary artery smooth muscle cells. \u0000 \u0000 \u0000Key words: \u0000Hypertension pulmonary; Anoxia; Receptors, calcium-sensing; Myocytes, smooth muscle; Mice","PeriodicalId":351524,"journal":{"name":"Chinese Journal of Neonatology","volume":"7 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121012233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0