Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies最新文献

Machine Learning and Rule Mining Techniques in the Study of Gene Inactivation and RNA Interference 基因失活和RNA干扰研究中的机器学习和规则挖掘技术

Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies Pub Date : 2019-04-23 DOI: 10.5772/INTECHOPEN.83470

Saurav Mallik, U. Maulik, Namrata Tomar, Tapas Bhadra, A. Mukhopadhyay, Ayan Mukherji

{"title":"Machine Learning and Rule Mining Techniques in the Study of Gene Inactivation and RNA Interference","authors":"Saurav Mallik, U. Maulik, Namrata Tomar, Tapas Bhadra, A. Mukhopadhyay, Ayan Mukherji","doi":"10.5772/INTECHOPEN.83470","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.83470","url":null,"abstract":"RNA interference (RNAi) and gene inactivation are extensively used biological terms in biomedical research. Two categories of small ribonucleic acid (RNA) molecules, viz ., microRNA (miRNA) and small interfering RNA (siRNA) are central to the RNAi. There are various kinds of algorithms developed related to RNAi and gene silencing. In this book chapter, we provided a comprehensive review of various machine learning and association rule mining algorithms developed to handle different biological problems such as detection of gene signature, biomarker, gene module, potentially disordered protein, differentially methylated region and many more. We also provided a comparative study of different well-known classifiers along with other used methods. In addition, we demonstrated the brief biological information regarding the immense biological challenges for gene activation as well as their advantages, disadvantages and possible thera- peutic strategies. Finally, our study helps the bioinformaticians to understand the overall immense idea in different research dimensions including several learning algorithms for the benevolent of the disease discovery.","PeriodicalId":336265,"journal":{"name":"Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133998957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies 调控基因表达——RNAi和CRISPR-Cas9技术的桥接

Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies Pub Date : 2019-02-06 DOI: 10.5772/intechopen.84229

Aditi Singh

引用次数: 26

Strand Displacement Amplification for Multiplex Detection of Nucleic Acids 核酸多重检测的链位移扩增技术

Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies Pub Date : 2018-11-19 DOI: 10.5772/INTECHOPEN.80687

Lingwen Zeng, Omar Mukama, Xuewen Lu, Shilin Cao, DonghaiLin

引用次数: 3

Nontransformative Strategies for RNAi in Crop Protection RNAi在作物保护中的非转化策略

Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies Pub Date : 2018-11-05 DOI: 10.5772/INTECHOPEN.80874

Deise Cagliari, E. A. D. Santos, N. Dias, G. Smagghe, M. Zotti

{"title":"Nontransformative Strategies for RNAi in Crop Protection","authors":"Deise Cagliari, E. A. D. Santos, N. Dias, G. Smagghe, M. Zotti","doi":"10.5772/INTECHOPEN.80874","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.80874","url":null,"abstract":"RNAi in crop protection can be achieved not only by plant-incorporated protectants through plant transformation (transgenic) but also by nontransformative strategies such as formulations of sprayable dsRNAs used as direct control agents, resistance fac- tor repressors, or developmental disruptors. Therefore, the RNAi-based biopesticides are expected to reach the market also in the form of nontransgenic strategies such as sprayable products, stem injection, root drenching, seed treatment, or powder/granule. While the delivery of dsRNA by transgenic expression is well established, it requires generations of crop plants and is costly, which may take years and delays for practical application, depending on the regulatory rules, plant transformability, genetic stabil- ity, and public acceptance of genetically modified crop species. DsRNA delivery as a nontransgenic approach was already published as a proof-of-concept work, so it is time to point out some directions on how the real potential for agriculture and crop protection is. phloem-feeding insects as and aphids, RNAs prove difficult to get into insect cell","PeriodicalId":336265,"journal":{"name":"Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies","volume":"238 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121354382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Gene Silencing Agents in Breast Cancer 乳腺癌中的基因沉默剂

Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies Pub Date : 2018-11-05 DOI: 10.5772/INTECHOPEN.79642

A. Qattan

{"title":"Gene Silencing Agents in Breast Cancer","authors":"A. Qattan","doi":"10.5772/INTECHOPEN.79642","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.79642","url":null,"abstract":"MicroRNAs (miRs) are a class of non-coding RNAs, approximately 20–25 nucleotides long, discovered in the nematode, Caenorhabditis elegans , in 1993. There are two primary categories of non-coding RNA (ncRNA): (1) short interfering RNAs (siRNA) and (2) microRNAs (miRs). In general, miRs control protein production via partially complementary binding of the mRNA 3′UTRs. Both siRNAs and miRNAs are critical regulators of developmental and homeostatic processes as well as disease pathogenesis. While the treatment of advanced stage breast cancer presents several challenges, the development of therapeutic resistance contributes to a high mortality rate. Dysregulation of miR expression has been implicated in progression of breast cancer disease. Moreover, miRs have been found to play a role in the development of drug resistance. In this context, one of the therapeutic potentials of miRNAs is the correlation of circulating miR levels with breast cancer progression stages and disease phenotypes. Secondly, researchers are investigat-ing novel delivery strategies for the substitution or silencing of ncRNAs involved in the disease. This chapter describes both the general miRNA mechanism of actions and the miRNAs related to breast cancer research. It is specifically designed for breast cancer researchers with expertise in gene delivery, clinicians, and clinical translational scientists.","PeriodicalId":336265,"journal":{"name":"Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies","volume":"110 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131353183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

CRISPR-ERA for Switching Off (Onco) Genes CRISPR-ERA用于关闭(Onco)基因

Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies Pub Date : 2018-11-05 DOI: 10.5772/INTECHOPEN.80245

M. Sánchez-Martín, I. García-Tuñón

引用次数: 3

MultiSite Gateway Technology Is Useful for Donor DNA Plasmid Construction in CRISPR/Cas9-Mediated Knock-In System 多位点网关技术在CRISPR/ cas9介导的敲入系统中构建供体DNA质粒的应用

Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies Pub Date : 2018-11-05 DOI: 10.5772/INTECHOPEN.80775

Takeshi Yasuda, Katsushi Tajima

引用次数: 1