International Journal of Biochemistry and Pharmacology最新文献

Effect of Clodronate Administration on the Structure of the Primary Spongiosa derived from the Growth Cartilage in Growing Rats 氯膦酸钠对生长大鼠生长软骨原代海绵组织结构的影响

International Journal of Biochemistry and Pharmacology Pub Date : 2019-11-13 DOI: 10.18689/IJBP-1000106

Ángela Fernández-Iglesias Rocío Fuente Laura Alonso-Duran Helena Gil-Peña, J. M. López

{"title":"Effect of Clodronate Administration on the Structure of the Primary Spongiosa derived from the Growth Cartilage in Growing Rats","authors":"Ángela Fernández-Iglesias Rocío Fuente Laura Alonso-Duran Helena Gil-Peña, J. M. López","doi":"10.18689/IJBP-1000106","DOIUrl":"https://doi.org/10.18689/IJBP-1000106","url":null,"abstract":"The effect of the inhibition of the resorptive activity of osteoclastic cells induced by bisphosphonate treatment on the primary spongiosa derived from the calcified cartilage of the growth plate was studied. We focused our attention on the primary spongiosa because it is the initial trabecular bone network that is first formed directly from growth plate mineralized cartilaginous septa. The study was carried out in male Sprague-Dawley rats at the age of 35 days, coinciding with the prepubertal growth spurt, a stage characterized by the highest values for growth rate. Animals were classified in two groups, controls and rats treated with clodronate 60 mg/kg/day. Body weights and tibial length were measured. The rate of longitudinal bone growth was obtained by calceine labelling and the height of the growth plate cartilage was measured. Histochemical analysis included Alcian blue staining, detection of tartrate-resistant acid phosphatise (TRAP) activity, von Kossa staining for mineralization and immunolocalization of proliferating cells. Microscopic examination revealed numerous tartrate-resistant acid phosphatase (TRAP)-positive cells at the chondroosseous junction and associated with subchondral trabeculae in control rat and that clodronate treatment induced a marked reduction of these cells. Clodronate-treated rats presented thinner subchondral trabeculae that were more irregularly oriented and decreased cell proliferation in the primary spongiosa. Results obtained showed that changes induced by clodronate treatment has little effect on the activity of the growth plate cartilage, without a significant effect on longitudinal bone growth even at doses much higher than those used in clinical practice.","PeriodicalId":332720,"journal":{"name":"International Journal of Biochemistry and Pharmacology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122163579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental and Therapeutic Trials of Amygdalin 苦杏仁苷的实验与治疗试验

International Journal of Biochemistry and Pharmacology Pub Date : 2019-10-28 DOI: 10.18689/ijbp-1000105

R. H. Salama, A. Ramadan, Tasneem A. Alsanory, M. Herdan, O. M. Fathallah, Aya A. Alsanory

引用次数: 13

Anti-Mitotic properties of Pawpaw, Pomegranate and Safflower 木瓜、石榴和红花的抗有丝分裂特性

International Journal of Biochemistry and Pharmacology Pub Date : 2019-09-09 DOI: 10.18689/ijbp-1000104

P. Waithaka, E. M. Gathuru, B. Githaiga, Damaris Nyawera

{"title":"Anti-Mitotic properties of Pawpaw, Pomegranate and Safflower","authors":"P. Waithaka, E. M. Gathuru, B. Githaiga, Damaris Nyawera","doi":"10.18689/ijbp-1000104","DOIUrl":"https://doi.org/10.18689/ijbp-1000104","url":null,"abstract":"Deaths due to cancer are on the increase all over the world. Drugs that are used in chemotherapy produce serious side effects on the patient. This study aimed at determining the anti-mitotic activity of pomegranate, pawpaw and safflower. The antimitotic activities are used a leads to finding the anticancer properties of crude extracts. The pomegranate, pawpaw and safflower samples were collected from Egerton University while the onions were collected from Njokerio market. The plant samples were dried under room temperature (23 ± 2°C). Extraction of crude extracts was carried out using petroleum ether, chloroform, ethyl acetate and methanol. The presence of glucosides, phenolics, flavonoids, coumarins, alkaloids, tannins and saponins was determined. The onion roots were trimmed using a sterile surgical blade. The onions were placed on bottles with the roots touching the crude extracts at dilutions of up to 104. The lengths of the roots were measured after 5 d. The root tips were separately macerated before preparation of temporally slides. The slides were observed for stages of mitotic cell division. There was no significant difference between the yield of the crude extracts in pomegranate, safflower and pawpaw (F=0.1295 P=0.8809). There were variations in the chemical compounds in the crude extracts of pomegranate, pawpaw and safflower. The length of onion roots after exposure to crude extracts from pomegranate, safflower and pawpaw varied significantly (F=32.8524 P=7.58E-05). The crude extracts from pomegranate, pawpaw and safflower interfered with mitotic cell division in onion roots. The crude extracts from pomegranate, pawpaw and safflower inhibited root development in onions. This suggested that the extracts have antitumor properties. There is need of testing the crude extracts against tumorous cells to determine whether they have the potential of curing or protecting against cancer.","PeriodicalId":332720,"journal":{"name":"International Journal of Biochemistry and Pharmacology","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123796433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preliminary Pharmacognostic Evaluations and Phytochemical Studies on Leaves of Bauhinia Racemosa (Sonapatti) 总状紫荆叶的初步生药学评价及植物化学研究

International Journal of Biochemistry and Pharmacology Pub Date : 2019-08-26 DOI: 10.18689/ijbp-1000103

N. Khan, M. Pande, K. Jha

引用次数: 0

Protective Effect of Lyophilization on Fermentative, Microbiological and Sensory Properties of Kefir 冻干对开菲尔发酵、微生物和感官特性的保护作用

International Journal of Biochemistry and Pharmacology Pub Date : 2019-07-19 DOI: 10.18689/ijbp-1000102

A. Moretti, R. Gamba, Maria do Céu Costa, G. Antoni, Á. Peláez

引用次数: 2

Analytical methods Development and Validation of Crizotinib by RP-HPLC Technique 克唑替尼的RP-HPLC分析方法的建立与验证

International Journal of Biochemistry and Pharmacology Pub Date : 2019-01-28 DOI: 10.18689/ijbp-1000101

P. Putta

引用次数: 2