Coffee and Cocoa Research Journal最新文献

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Morphological and Molecular Identification of Helopeltis Species on Cocoa from Kaliwining Experimental Station, Jember, Indonesia 印度尼西亚卡利温宁试验站可可上Helopeltis的形态和分子鉴定

Coffee and Cocoa Research Journal Pub Date : 2023-08-24 DOI: 10.22302/iccri.jur.pelitaperkebunan.v39i2.552

Gracia Melsiana Aldini, Anisa Aulia Rahma, Aris Budiman

{"title":"Morphological and Molecular Identification of Helopeltis Species on Cocoa from Kaliwining Experimental Station, Jember, Indonesia","authors":"Gracia Melsiana Aldini, Anisa Aulia Rahma, Aris Budiman","doi":"10.22302/iccri.jur.pelitaperkebunan.v39i2.552","DOIUrl":"https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v39i2.552","url":null,"abstract":"Severe crop loss of cocoa in Indonesia has long been reported to be caused by Helopeltis. It has been noted that cocoa and Helopeltis have existed in Kaliwining Experimental Station of Indonesian Coffee and Cocoa Research Institute (ICCRI), Jember, Indonesia, for about 100 years, and the species have developed during that time. Our study aimed for examining the Helopeltis species that attacked cocoa in this experimental station both morphologically, morphometrically, and molecularly and to compare the species with the one attacking cocoa in Java and Nusa Tenggara, preserved at the GenBank. Helopeltis was sampled from infested cocoa pods collected representatively from cocoa at the Kaliwining ES and then reared in the Crop Protection Laboratory of ICCRI. Adults of Helopeltis laboratory-reared insects were identified, 30 females 30 males were selected. Weexamined the morphology and morphometry of the samples, and their identifica tion was based on an insect identification key. Morphometry measurement of male and female body parts includes body length, antenna segments, head, eyes, collar, wing, tibia, and femur. In addition, the molecular identification of Helopeltissp. was made through DNA extraction, amplification, and sequence analysis of DNA target regions using the COI part of mitochondrial DNA. External morphological identification, supported by morphometry of the Helopeltis specimen from Kaliwning, the white bands on all femora, body length, and the ratio of the firstantennal segment to the posterior width of the pronotum suggested that the insect belongs to Helopeltis bradyi. Similarly, molecular identification using PCR amplification through specific primers from COI gene sequences confirmed that Helopeltis specimens from Kaliwining are classified as Helopeltis bradyi.","PeriodicalId":30674,"journal":{"name":"Coffee and Cocoa Research Journal","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135466012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Coconut Shell Biochar and Rhizobacteria Consortium to Increase Cocoa Production (Theobroma cacao L.) 椰壳生物炭和根瘤菌联合体在可可增产中的应用

Coffee and Cocoa Research Journal Pub Date : 2023-08-24 DOI: 10.22302/iccri.jur.pelitaperkebunan.v39i2.545

Reynaldi Laurenze, Ambo Ala, Ifayanti Ridwan Saleh

{"title":"Application of Coconut Shell Biochar and Rhizobacteria Consortium to Increase Cocoa Production (Theobroma cacao L.)","authors":"Reynaldi Laurenze, Ambo Ala, Ifayanti Ridwan Saleh","doi":"10.22302/iccri.jur.pelitaperkebunan.v39i2.545","DOIUrl":"https://doi.org/10.22302/iccri.jur.pelitaperkebunan.v39i2.545","url":null,"abstract":"The decline in Indonesia’s cocoa production as a result of soil fertility degra dation causes fertilization to become one of effective effects. Because of that, it is possible to restore soil fertility potential by using soil fertility enhancer biochar combined with rhizobacteria consortium to create suitable conditions for plant.This research was aimed to study and investigate coconut shell biochar interacts with rhizobacteria in increasing cocoa production. This research was conducted at Kodeoha, North Kolaka, Southeast Sulawesi from September 2022 until March 2023. A split plot design with three replications each consisted of two experimental units was used as an experimental design. Biochar dosage as the main plot consisted of four levels, namely 0 ton ha-1 (control), 2.5 ton ha-1, 5 ton ha-1, and 7.5 ton ha-1 and rhizobacteria concentration as the sub plots consisted offour levels, namely 0 mL L-1, 50 mL L-1, 100 mL L-1, and 150 mL L-1. Results of this research showed a significant interaction between biochar 5 tons ha-1 and rhizobacteria 100 mL L-1 which provide best result for chlorophyll a (336 μmol m-2), chlorophyll b (150 μmol m-2), and total chlorophyll (484 μmol m-2). The interaction between biochar 5 tons ha-1 and rhizobacteria 150 mL L-1 showed the best results for number of harvested pods per tree (10.00 pods), bean weight per tree (606 g trees-1), stomata opening area (31 μm2). The interaction between biochar 7.5 tons ha-1 and rhizobacteria 150 mL L-1 showed the best results on the weight of 100 dry beans at water content 8% (159.03 g)","PeriodicalId":30674,"journal":{"name":"Coffee and Cocoa Research Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135466011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0