{"title":"Harnessing the Genetic Diversity and Metabolic Potential of Extremophilic Microorganisms through the Integration of Metagenomics and Single-Cell Genomics","authors":"D. Goyal, S. Swaroop, J. Pandey","doi":"10.5772/intechopen.82639","DOIUrl":"https://doi.org/10.5772/intechopen.82639","url":null,"abstract":"Microorganisms thriving under extreme environments have proven to be an invaluable resource for metabolic products and processes. While studies carried out on microbial characterization of extremophilic environments during golden era of microbiology adapted a ‘reductionist approach’ and focused on isolation, purification and characterization of individual microbial isolates; the recent studies have implemented a holistic approach using both culture-dependent and culture-independent approaches for characterization of total microbial diversity of the extreme environments. Findings from these studies have unmistakably indicated that microbial diversity within extreme environments is much higher than anticipated. Consequently, unraveling the taxonomic and metabolic characteristics of microbial diversity in extreme environments has emerged as an imposing challenge in the field of microbiology and microbial biotechnology. To a great extent, this challenge has been addressed with inception and advancement of next-generation sequencing and computing methods for NGS data analyses. However, further it has been realized that in order to maximize the exploitation of genetic and metabolic diversity of extremophilic microbial diversity, the metagenomic approaches must be combined synergistically with single-cell genomics. A synergistic approach is expected to provide comprehensions into the biology of extremophilic microorganism, including their metabolic potential, molecular mechanisms of adaptations, unique genomic features including codon reassignments etc.","PeriodicalId":292446,"journal":{"name":"Extremophilic Microbes and Metabolites - Diversity, Bioprospecting and Biotechnological Applications","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130855415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Review on Electro-Rheological Fluid (ER) and Its Various Technological Applications","authors":"S. Datta, R. Barua, Jonali Das","doi":"10.5772/intechopen.90706","DOIUrl":"https://doi.org/10.5772/intechopen.90706","url":null,"abstract":"The technology of electro-rheological fluids is old, but now it is being used in market at high speed. In the near future, ER fluids may be used for structure design where accuracy, density and power density are primarily the main criteria. For minimizing the costs and maximizing the functionality of the products, like where the viscosity of the fluid is varied to design, solid structure ER fluids are used. Features like fast response and easy interface between electrical and mechanical input–output makes the ER fluid attractive to various technology fields. In this study, ER fluids’ working principle, various low-cost ER fluids working procedures and ER fluid applications in multiple areas are explained.","PeriodicalId":292446,"journal":{"name":"Extremophilic Microbes and Metabolites - Diversity, Bioprospecting and Biotechnological Applications","volume":"123 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126975848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Methanogenic Diversity and Taxonomy in the Gastro Intestinal Tract of Ruminants","authors":"F. Faridi, S. Khan","doi":"10.5772/INTECHOPEN.82829","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.82829","url":null,"abstract":"To elucidate the microbial dynamics inside rumen of animals of livestock importance and to provide a better ration to them in order to control various metabolic disorders, a better understanding of the rumen microbial ecology is pivotal. The fundamental knowledge of methanogenic population inside gut environment and ruminal fermentation is of considerable importance as it has a significant impact on the various metabolic activities of the animal. The major methanogens isolated and characterized from ruminants like cattle, sheep, steers, goats, reindeers are from the order Methanobacteriales, Methanomicrobiales, Methanococcales, Methanosarcinales and Methanomassiliicoccales. The chapter deals with present knowledge available regarding the methanogenic diversity present in the gastro-intestinal tract of ruminants all over the world primarily through constructing 16S rRNA gene clone libraries and tries to uncover the new genera in ruminant’s microbiome and their adaptations in extreme environment. To get a better idea regarding the composition of methanogen community, further studies are required in relation to the effect of diet and animal species to the rumen methanogens.","PeriodicalId":292446,"journal":{"name":"Extremophilic Microbes and Metabolites - Diversity, Bioprospecting and Biotechnological Applications","volume":"83 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121374571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. J. Rivera-Rivera, V. Acosta‐Martínez, E. Cuevas
{"title":"Tree Species and Precipitation Effect on the Soil Microbial Community Structure and Enzyme Activities in a Tropical Dry Forest Reserve","authors":"M. J. Rivera-Rivera, V. Acosta‐Martínez, E. Cuevas","doi":"10.5772/INTECHOPEN.82579","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.82579","url":null,"abstract":"We examined the soil microbial community structure and soil enzyme potential within three dominant tree species at a tropical dry forest during five months. Changes within microbial community in response to sampling periods and tree species were evaluated using fatty acid methyl-ester and enzymes potential. We found that both tree species and precipitation determined microbial community structure and enzyme potential. This is the first study that provides insight into the soil microbial community at Guánica Dry Forest, a valuable contribution that will help elucidate strategies for better management and protection of the soil biota of the area.","PeriodicalId":292446,"journal":{"name":"Extremophilic Microbes and Metabolites - Diversity, Bioprospecting and Biotechnological Applications","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124557063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Á. Aguilera, G. de Diego-Castilla, Susana Osuna, R. Bardera, Suthyvann Sor Mendi, Y. Blanco, E. González-Toril
{"title":"Microbial Ecology in the Atmosphere: The Last Extreme Environment","authors":"Á. Aguilera, G. de Diego-Castilla, Susana Osuna, R. Bardera, Suthyvann Sor Mendi, Y. Blanco, E. González-Toril","doi":"10.5772/INTECHOPEN.81650","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.81650","url":null,"abstract":"The atmosphere is an extreme environment where organisms are subject to low temperatures and high radiation. Many of the microorganisms detected there appear in resistant forms or show mechanisms of adaptation designed to withstand these extreme conditions. Airborne microorganisms may play an important role in the global climate system, biogeochemical cycling, and health. Dust storms are the atmospheric phenomenon that move more topsoil through the Earth’s atmosphere, and numerous microorganisms attached to dust particles are thus transported. The Iberian Peninsula is periodically affected by this phenomenon as African dust frequently reaches southern Europe and the Mediterranean basin. There are numerous methods for sampling airborne microbes, but factors such as low biomass and high variability of the atmosphere render them not yet sufficiently efficient. Very few studies have been conducted directly in the atmosphere via sampling using airborne platforms. The National Institute for Aerospace Technology has two CASA C-212-200 aircraft that have been suitably modified to operate as airborne research platforms. These aircraft are a unique tool for the study of atmospheric microbial diversity and the different environments where they can be found. A study of the airborne microbial diversity in a Saharan dust event from four aerobiology sampling flights is provided in advance.","PeriodicalId":292446,"journal":{"name":"Extremophilic Microbes and Metabolites - Diversity, Bioprospecting and Biotechnological Applications","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121330079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Predator-Prey Interactions in Ciliated Protists","authors":"F. Buonanno, C. Ortenzi","doi":"10.5772/INTECHOPEN.78622","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.78622","url":null,"abstract":"Protists appeared relatively early in evolution, about 1.8 billion years ago, soon after the first prokaryotic organisms. During this time period, most species developed a variety of behavioral, morphological, and physiological strategies intended to improve the ability to capture prey or to avoid predation. In this scenario, a key role was played by specialized ejectable membrane-bound organelles called extrusomes, which are capable of discharging their content to the outside of the cell in response to various stimuli. The aim of this chapter is to describe the two main strategies adopted in ciliate predator-prey interactions: (a) the first is mediated by mechanical mechanisms and involves, for example, extrusomes called trichocysts and (b) the second is mediated by toxic secondary metabolites and involves different kinds of chemical extrusomes.","PeriodicalId":292446,"journal":{"name":"Extremophilic Microbes and Metabolites - Diversity, Bioprospecting and Biotechnological Applications","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134015363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}