ChemRN: Analytical Chemistry Applications (Topic)最新文献

筛选

英文中文

Native Mass Spectrometry and Gas-Phase Fragmentation Provide Rapid and In-Depth Topological Characterization of a PROTAC Ternary Complex 原生质谱和气相破碎提供快速和深入的拓扑表征PROTAC三元配合物

ChemRN: Analytical Chemistry Applications (Topic) Pub Date : 2021-01-15 DOI: 10.2139/ssrn.3767161

J. Song, Nicole D. Wagner, Jing-Xiao Yan, Jing Li, Richard Y.-C. Huang, A. Balog, J. Newitt, Guodong Chen, M. Gross

{"title":"Native Mass Spectrometry and Gas-Phase Fragmentation Provide Rapid and In-Depth Topological Characterization of a PROTAC Ternary Complex","authors":"J. Song, Nicole D. Wagner, Jing-Xiao Yan, Jing Li, Richard Y.-C. Huang, A. Balog, J. Newitt, Guodong Chen, M. Gross","doi":"10.2139/ssrn.3767161","DOIUrl":"https://doi.org/10.2139/ssrn.3767161","url":null,"abstract":"Proteolysis-targeting chimeras (PROTACs) represent a new direction in small-molecule therapeutics whereby a heterobifunctional linker to a protein of interest (POI) induces its ubiquitination-based proteolysis by recruiting an E3 ligase. Here, we show that charge reduction, native mass spectrometry, and gas-phase activation methods combine for an in-depth analysis of a PROTAC-linked ternary complex. Electron capture dissociation (ECD) of the intact POI-PROTAC-VCB complex (a trimeric subunit of an E3 ubiquitin ligase) promotes POI dissociation. Collision-induced dissociation (CID) causes elimination of the nonperipheral PROTAC, producing an intact VCB-POI complex not seen in solution but consistent with PROTAC-induced protein-protein interactions. In addition, we used ion mobility spectrometry (IMS) and collisional activation to identify the source of this unexpected dissociation. Together, the evidence shows that this integrated approach can be used to screen for ternary complex formation and PROTAC-protein contacts and may report on PROTAC-induced protein-protein interactions, a characteristic correlated with PROTAC selectivity and efficacy.","PeriodicalId":286393,"journal":{"name":"ChemRN: Analytical Chemistry Applications (Topic)","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123379961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Colorimetric Analysis to Predict Ph Value of the Water on Mpads (Microfluidic Paper Based Analytical Devices) Testzone Area 用比色法预测Mpads(微流控纸基分析装置)测试区水的Ph值

ChemRN: Analytical Chemistry Applications (Topic) Pub Date : 2020-11-24 DOI: 10.2139/ssrn.3736534

Boddapati Hemamadhusri, Dr. Ramesh Kumar Panneerselvam

{"title":"Colorimetric Analysis to Predict Ph Value of the Water on Mpads (Microfluidic Paper Based Analytical Devices) Testzone Area","authors":"Boddapati Hemamadhusri, Dr. Ramesh Kumar Panneerselvam","doi":"10.2139/ssrn.3736534","DOIUrl":"https://doi.org/10.2139/ssrn.3736534","url":null,"abstract":"Microfluidic paper-based analytical devices (μPADs) are the futuristic microfluidic paper-based analytical diagnostic devices that are utilized to perform the laboratory-based test (ex: water, blood, urine, etc..,) at the user location. Microfluidic paper-based analytical devices (μPADs) are known as a robust analytical platform that can fulfil the stipulations. This analysis aims to offer a detailed assessment of the μPADs that are advanced for the estimation of vital water pH values. The significant challenge for users with the μPAD based diagnostic is that, the user is unable to evaluate the precise outcome of the diagnostic result. Here, the water pH diagnostic test is considered on the μPADs, since water pollutant is a severe environmental hassle that affect thousands of humans and as a result it increases the demand for common water first-class monitoring. The necessity for analytical systems that merge extreme sensibleness, selectivity and accuracy with low cost, portability and consumer friendliness remains as a prominent part. This research work proposes a technique to detect the pH value of the water by acquiring the images of the tested μPADs, extract the shape of the test zone area and perform colourimetric analysis to determine the pH value of the test zone area on scale 0-13. The main advantages of the μPADs are comparatively low cost and accessible abundance, reduce the overall cost of testing, and reduce the number of visits to the research laboratory.","PeriodicalId":286393,"journal":{"name":"ChemRN: Analytical Chemistry Applications (Topic)","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115221189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of the Thermal Conductivity by Using the Hot Wire Method: Theory, Simulation and Experiment 热丝法测定导热系数:理论、模拟与实验

ChemRN: Analytical Chemistry Applications (Topic) Pub Date : 2020-10-17 DOI: 10.46382/mjbas.2020.4309

Giovanni Alcocer

{"title":"Determination of the Thermal Conductivity by Using the Hot Wire Method: Theory, Simulation and Experiment","authors":"Giovanni Alcocer","doi":"10.46382/mjbas.2020.4309","DOIUrl":"https://doi.org/10.46382/mjbas.2020.4309","url":null,"abstract":"The measurement of the physical properties (density, viscosity, surface tension, thermal conductivity, etc) is of great importance to the research industry and for the physical, chemical and biomedical applications. The thermal conductivity is a measurement of the material’s ability to conduct heat. The transient hot wire method is a suitable method to measure the thermal conductivity due to its very cheap cost of construction, accuracy and because it is a fast method of measurement. The implementation requires accurate temperature sensing, automatic control, data acquisition and data analysis. The basic procedure consists of measuring the temporal temperature rise in a thermoresistance (thin wire) immersed in the solution by applying an electrical current in the wire. Therefore, the wire works as a heat source and a temperature sensor. The time of measurement is very short and therefore the convection effect could be minimized. Then, the heat transfer to the infinite medium is due only to the conduction transfer effect. The thermal conductivity can be determined from the slope of the curve ΔT versus ln(t) due to the linear relation between ΔT and ln(t).","PeriodicalId":286393,"journal":{"name":"ChemRN: Analytical Chemistry Applications (Topic)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120953657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2