The 6th International Conference of Biotechnology, Environment and Engineering Sciences最新文献

{"title":"Detection of Cytomegalovirus by ELISA Technique in Rheumatoid Arthritis Patients in Mosul / Iraq","authors":"Anmar A.ALTaie, Momammed A.Ibraheem, Momammad A. Muhammad","doi":"10.46617/icbe6011","DOIUrl":"https://doi.org/10.46617/icbe6011","url":null,"abstract":": In the present study, the association between CMV infection and rheumatoid arthritis was examined. A cohort of 92 serum samples were recruited from rheumatoid arthritis patients at age group of (30-62) years (25 males, 47females) and 20 from healthy control. ELISA technique was used to assess the levels of CMV (IgM) and CMV (IgG) antibodies. Results showed that rheumatoid arthritis patients blood groups are as follow: 29.16% (AB+), 25 % (A+), 22.22 % ( O+), 19.44 % ( B+), 1.39 %( A-), 2.78 % (B-), and 1.39 % (AB-), and 0 % (O-). According to gender the results showed the highest ABO blood groups in females with AB (20.83%), and A (19.44%) while blood group O – has been not recorded. The results of Rh phenotype tests in patients with rheumatoid arthritis were Rh + 94.43% and Rh – 5.56%. CMV (IgG and IgM) assay using ELISA technique shows that 6 patients had seropostively of IgM against CMV antigen was apportioned as 2 patients with each blood groups A, B and AB while the infection has not detected with blood group O. In addition CMV (IgG), revealed that 24 patients has seropositively, apportioned as 8 (12.8 IU/ml) at blood group AB, 6 (11.1 IU/ml) at blood group B , 6(10.2 IU/ml) at blood groups A while in blood group O was 4 (12.9 IU/ml).According to sex , the results showed that highest titers of CMV IgG in females were 14 patients (14.3 I/ml) and in males 5 patients (10.9 IU/ml).The current study revealed no significant correlation between levels of IgG, IgM and CMV infections in rheumatoid arthritis patients. On other hand, there was weak significant correlation between blood groups and rheumatoid arthritis patients.","PeriodicalId":267855,"journal":{"name":"The 6th International Conference of Biotechnology, Environment and Engineering Sciences","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114666783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Zinc Oxide (ZnO) Nanoparticles in Germination and Growth of Chickpea (Cicer arietinum L.) Plant Seedlings Under Water Stress Effect.","authors":"Rana Tariq Yahea, A. Mohammed","doi":"10.46617/icbe6008","DOIUrl":"https://doi.org/10.46617/icbe6008","url":null,"abstract":"The current study searched the effect of Zinc oxide (ZnO) nanoparticles with concentrations 0.5,1.0 and 2.0 mgL -1 on the seeds germination ofchickpea Cicer arietinum plant and their seedlings growth under the effect of water stress with the presence the Poly Ethylene Glycol (PEG) compound at 0.5, 1.0 and 2.0 mgL -1 concentrations. The results showed the inhibitor role of PEG when it's found once in the medium at all concentrations on the percentage of seeds germination, where the higher reached to 35% in the presence of 2.0 mgL -1 compared with 60% in the MS alone(control). While the addition of zinc oxide nanoparticles signally to the medium led to encouraged the germination reached the best of them to 75% at 2.0 mgL -1 ,the characteristic results are the ability of these nanoparticles at 2.0 mg L -1 to reduce the inhibitor effect of PEG compound and the seedlings give perfect differences in their length and","PeriodicalId":267855,"journal":{"name":"The 6th International Conference of Biotechnology, Environment and Engineering Sciences","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126073648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of cytotoxic levels of exotoxin A ofPseudomonas aeruginosa cultivated on simple synthetic medium","authors":"Ibtesam Ghadban Auda, Afrah Fahad Abdulkareem","doi":"10.46617/icbe6005","DOIUrl":"https://doi.org/10.46617/icbe6005","url":null,"abstract":"The leading microorganism of chronic otitis media is P. aeruginosa and the most powerful exotoxin of P. aeruginosais exotoxin Athat responsible for the cytopathic effect on the host tissues. This study aimed to determine the percentage of toxA gene occurrences on chromosome of the otitis media most prevalentcausative agent, P. aeruginosa, study the effect of simple salts-based synthetic medium on the production of exotoxin A and evaluate the effect of produced exotoxin A amounts on mice vital organs. The results showed thatP. aeruginosa is predominant in otitis media as compared with the others. Furthermore, exotoxin A gene is found on all tested P. aeruginosa isolates. Therefore, all toxA positive P. aeruginosa isolates were subjected to produce exotoxin A by the cultivation of them on simple synthetic media(with trace iron and omitting the glutamate)and quantification of the exotoxin A by ELISA technique. Pseudomonas aeruginosa isolates were varied in exotoxin A production and were ranged from 78 to 1380 pg/ml. Injection ofpure exotoxin A at low doses within the produced values to mice showed histopathological lesions of mice vital organs; these lesions increase with increasing the injected dose.As a conclusion, P. aeruginosa is still in the front of otitis media pathogens and toxA gene is predominant on their chromosomes. Even in poor nutrition conditions and the presence of trace amounts of iron, P. aeruginosa isolates are still able to grow and produce exotoxin A at amounts can exert its harmful effect on mice vital organs.","PeriodicalId":267855,"journal":{"name":"The 6th International Conference of Biotechnology, Environment and Engineering Sciences","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125018260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The patentability of biotechnology inventions","authors":"Benayad Djalila","doi":"10.46617/icbe6012","DOIUrl":"https://doi.org/10.46617/icbe6012","url":null,"abstract":"The patentability of biotechnology inventions Benayad Djalila Faculty of Law and Political Science, University of Mhamed Bougherra ,Boumerdes , Algeria ania-rose14@hotmail.fr صخلم : يهف ن و و يللآا ، ناو ىلع و يف . نإ يه وأ يف اهنأ ىلع يف و ، يهف عم ا ىلع لجا و اهتافصو . ف ب ا س ت خ د ا م ایجولونكتلا ةیویحلا ، و ، يف ... خلا لك ن اهللاخ و يف هنإف ناك عضو ، ا ام لاخ ل اهب . لولأا","PeriodicalId":267855,"journal":{"name":"The 6th International Conference of Biotechnology, Environment and Engineering Sciences","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128487948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Flash Flood Hazard Degrees of Wadi EL Tarfa, Eastern Desert, Egypt: Using GIS Based Morphometric Analysis Technique.","authors":"Mohamed Abu Setta, H. Garamoon, Ashraf R. Shabana","doi":"10.46617/icbe6004","DOIUrl":"https://doi.org/10.46617/icbe6004","url":null,"abstract":": Wadi EL Tarfa basin is one of the largest basins in the Eastern Desert of Egypt where its surface area reaches 4939 Km 2 . It is considered one of the promising agricultural reclamation areas in Egypt. Wadi EL Tarfa is mainly running on El Maaza limestone plateau and drains in the Nile River at the southeast of BeniMazar City. The morphometric analyses of Wadi El Tarfa was carried out through ARC GIS software 10.2 based on DEM with resolution of 30 m (SRTM 1 Arc-second Global) which downloaded online from the USGS site. Wadi El Tarfa basin is subdivided into eight main sub-basins (from 1 to 8). According toStrahler ordering method, Wadi EL Tarfa attains eighth order and its sub-basins range between fifth order for small sub-basins (nos.1, 3, 4& 6) and seventh order for large sub-basins (nos. 7 & 8). The flash flood hazard degrees of El Tarfa basin have been estimated depending on the morphometric parameters which have direct effect on the flood amount. The morphometric ranking method was used in determining the hazard degrees which are divided into five hazard classes. All sub-basins are classified according to the degrees of hazard. The classification shows that the sub-basin (no. 2) has the lowest hazard while sub-basins (nos. 3, 5, 6& 8) are the most hazardous. The resultant flash flood risk maps indicate the most probable sub-basins and the main roads expected to be affected by flood hazard. It is recommended to construct protection systems in Wadi El Tarfa area such as retardation earthen dams to mitigate the floods risk in one hand and to increase the rain water harvesting on the other hand. Abstract: Leuconostoc species are gram-positive, non-motile, non–spore-forming, hetero-fermentative, facultative cocci that are found in the environment. They have economic importance because of their use in the dairy and pickling industries and in wine making. Leuconostoc species are also found in fermented sausages, vacuum-packed meat products, cereals, and dairy products (butter, cream, fresh/raw milk, cheese) and causes diseases for human like brain abscess and urinary tract infections which are very similar in the shape like streptococcus and other related genera. We were collected 41 midstream urine samples from 14 Male and 27 Female patients came to ALKHNSAA Teaching Hospital and Al-Salam Teaching Hospital in Mosul city-IRAQ. We obtained only 3 (7.3%) of Leuconostoc spp. out of 41 according to identification tests, biochemical tests and resistance to vancomycin which is important test for this bacteria. We were ability of Leuconostoc spp. to grow in different temperature and different media. Leuconostoc nutrient agar 10 ° C can growth 25 ° C on both blood agar without hemolysis and Leuconostoc Selective media (LSM). Leuconostoc spp. were sensitive to IPM 10 (32mm/disk) and CIP 10 (27mm/disk), respectively and all of them were resistance against other antibiotics under study (AM 10 , E 15 , CN 10 , P 10 , VA 10 and TMP 10 ).","PeriodicalId":267855,"journal":{"name":"The 6th International Conference of Biotechnology, Environment and Engineering Sciences","volume":"86 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114395309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of copper metal presence in cattle fodder, milk, hair and manure in Malta","authors":"Hend Abeidi, D. Suda, E. Attard, E. Sinagra","doi":"10.46617/icbe6001","DOIUrl":"https://doi.org/10.46617/icbe6001","url":null,"abstract":": This study investigated the concentration of copper (Cu) present during the lifecycle of cattle in Malta to determine how metals enter and exit the animal, and their impact via the food chain on the human consumer. The determination of Cu in 10 cows from a dairy cattle farm located in Salina, Malta was estimated by taking milk (100), hair (100), fodder (15) and manure (10) samples over 10 weeks between September and November 2016 and their Cu concentration was determined using atomic absorption spectroscopy. Conventional methods were used to prepare all these samples. Cu concentration in milk samples showed high levels (0.02 to 0.07mg/L) which is higher than the maximum level recommended (0.01mg/L) by FAO and WHO. Cu concentration was significantly higher in summer than in autumn. However, hair samples results (3.04–6.88mg/kg) did not provide a clear impact by season. The concentration of Cu in different fodder types varied significantly ( 1.08 – 16.06 mg/Kg ). Manure Cu concentrations ranged from 10.70to 16.63 mg/kg but there were no distinctions between weeks or seasons. Considering the discrepancy of copper concentrations at inputs (feed) and outputs (manure, hair, milk) for a dairy cow, it can be concluded that there are other factors contributing to the copper concentration within the cow system.","PeriodicalId":267855,"journal":{"name":"The 6th International Conference of Biotechnology, Environment and Engineering Sciences","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125446615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of primary sedimentation tank on wastewater treatment plant units using computer simulation program","authors":"A. M. Hussein, Dhiaa Neama Jabbar, Shereen Qasim Abdulridha","doi":"10.46617/icbe6003","DOIUrl":"https://doi.org/10.46617/icbe6003","url":null,"abstract":": This study presents a computer program designed in Visual Basic 6 program. The capability of this program is to design wastewater treatment plant units and determination of its construction cost. The program deals with two types of wasted sludge the first type is wasted sludge from the aeration basin, while the second system is wasted sludge from the secondary clarifier. Also, there is a detailed output shows the design flowrate, mass balance analysis and composition of each stream in and out of each treatment unit. Program results are verified with hand calculations within the allowable recommended values and they showed a good accuracy. The volume of aeration basin and secondary sedimentation tank increased with the elimination of primary sedimentation tank from the design of the wastewater treatment plant. Generally, an increment of about (52 – 170) % for aeration basins and (58-185) % for secondary sedimentation tanks are observed in the present study. From other hand, gravity thickener volume is decreased by (29 - 42) %, anaerobic digester volume is decreased about (38 - 52) % and drying beds is decreased by (29 - 38) % approximately. The cost analysis showed that the cost of a wastewater treatment plant is increased after a certain amount of influent. That amount is nearly (36,708.33) m 3 /d when wasted sludge is taken from aeration basin and (24,833.33) m3/d when wasted sludge is taken from secondary clarifier .","PeriodicalId":267855,"journal":{"name":"The 6th International Conference of Biotechnology, Environment and Engineering Sciences","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124121881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}