Biocatalysis and Agricultural Biotechnology: Fundamentals, Advances, and Practices for a Greener Future最新文献

Immobilization Biocatalysis 固定化生物催化

Biocatalysis and Agricultural Biotechnology: Fundamentals, Advances, and Practices for a Greener Future Pub Date : 2018-10-03 DOI: 10.1201/9781351167444-11

引用次数: 0

Plant Nutrition and Phytohormones 植物营养与植物激素

Biocatalysis and Agricultural Biotechnology: Fundamentals, Advances, and Practices for a Greener Future Pub Date : 2018-10-03 DOI: 10.1201/9781351167444-20

引用次数: 0

Plant Tissue Culture 植物组织培养

Biocatalysis and Agricultural Biotechnology: Fundamentals, Advances, and Practices for a Greener Future Pub Date : 2018-10-03 DOI: 10.1201/9781351167444-19

S. Uma, Lakshmi, Saraswathi, Akbar, Mustaffa

{"title":"Plant Tissue Culture","authors":"S. Uma, Lakshmi, Saraswathi, Akbar, Mustaffa","doi":"10.1201/9781351167444-19","DOIUrl":"https://doi.org/10.1201/9781351167444-19","url":null,"abstract":"Plant regeneration through somatic embryogenesis from immature and mature zygotic embryos of Musa acuminata ssp. burmannica Abstract A simple and efficient protocol has been developed for in vitro regeneration of M. acuminata ssp. burmannica (AA) plants. Somatic embryos were produced when immature and mature zygotic embryo explants were cultured on Mura-shige and Skoog medium supplemented with plant growth regulators 2,4-dichlorophenoxyacetic acid; (2,4-D), picloram or benzyl adenine and indole acetic acid. In general, immature embryos responded better than mature embryos. Callus proliferation was highest in medium supplemented with 2,4-D (4.5 μM). Subsequent transfer of callus to fresh medium produced rapidly proliferating embryogenic calli. Embryo-genic calli were maintained in complete darkness for 15 d followed by cycles of 8 h dark and 16 h light, under white fluorescent lamps with a light intensity of 3,000 lm/m 2 and at temperature of 28±2°C. Regeneration of embryogenic calli into plantlets was higher for immature embryos (76.6%) than for mature embryos (50.6%). This plant regeneration protocol using mature or immature zygotic embryos, via somatic em-bryogenesis, has significant potential to improve germination efficiencies of hybrid progenies used in conventional breeding strategies. Furthermore, tests on seed storage showed that seed viability rapidly decline after harvesting and was negligible after 9 mo of storage. This indicates using freshly harvested seeds as explant material is necessary for maximizing the tissue culture response.","PeriodicalId":250916,"journal":{"name":"Biocatalysis and Agricultural Biotechnology: Fundamentals, Advances, and Practices for a Greener Future","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132649370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Agricultural Biotechnology, Introduction, and History 农业生物技术，介绍和历史

Biocatalysis and Agricultural Biotechnology: Fundamentals, Advances, and Practices for a Greener Future Pub Date : 2018-10-03 DOI: 10.1201/9781351167444-17

引用次数: 0

Plant Breeding and Seed Technology 植物育种与种子技术

Biocatalysis and Agricultural Biotechnology: Fundamentals, Advances, and Practices for a Greener Future Pub Date : 2018-10-03 DOI: 10.1201/9781351167444-18

引用次数: 0

Use of Enzymes in Industry 酶在工业中的应用

Biocatalysis and Agricultural Biotechnology: Fundamentals, Advances, and Practices for a Greener Future Pub Date : 2009-03-01 DOI: 10.1142/9781848162310_0016

P. Grunwald

引用次数: 1

Enzymes in Non-Conventional Media 非常规培养基中的酶

Biocatalysis and Agricultural Biotechnology: Fundamentals, Advances, and Practices for a Greener Future Pub Date : 2009-03-01 DOI: 10.1142/9781783269099_0014

P. Grunwald

引用次数: 3