International Archives of Orthopedic Surgery最新文献

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Transforming Growth Factor-Beta (TGF-Β) Regulates Nerve Growth Factor and Cyclooxygenase-2 Expression in Subsynovial Connective Tissue in Patients with Carpal Tunnel Syndrome 转化生长因子-Β (TGF- -Β)调节腕管综合征滑膜下结缔组织中神经生长因子和环氧合酶-2的表达

International Archives of Orthopedic Surgery Pub Date : 2018-12-31 DOI: 10.23937/IAOS-2017/1710002

Nakawaki Mitsufumi, Uchida Kentaro, Onuma Kenji, Sukegawa Koji, Matsumoto Toshihide, Inoue Gen, Sekiguchi Hiroyuki, Takaso Masashi

{"title":"Transforming Growth Factor-Beta (TGF-Β) Regulates Nerve Growth Factor and Cyclooxygenase-2 Expression in Subsynovial Connective Tissue in Patients with Carpal Tunnel Syndrome","authors":"Nakawaki Mitsufumi, Uchida Kentaro, Onuma Kenji, Sukegawa Koji, Matsumoto Toshihide, Inoue Gen, Sekiguchi Hiroyuki, Takaso Masashi","doi":"10.23937/IAOS-2017/1710002","DOIUrl":"https://doi.org/10.23937/IAOS-2017/1710002","url":null,"abstract":"Background: It has been reported that transforming growth factor-beta (TGF-β) is elevated in the subsynovial connec tive tissues (SSCTs) of patients with idiopathic carpal tunnel syndrome (CTS) and TGF-β induced fibrosis of SSCTs. A TGF-β-mediated pathway may be a potential target for CTS therapy. Cycloxygenase-2 (COX-2) and nerve growth factor (NGF) are regulated by TGF-β and are associated with fi brosis in several tissues; however, regulation in SSCTs has not been fully clarified. Methods: Forty-three SSCTs were sampled from 35 pa tients undergoing carpal tunnel release (CTR) surgery. Cor relation between TGF-β mRNA and protein expression and COX-2 and NGF were evaluated using quantitative PCR (q-PCR) analysis of total RNA sampled from the SSCTs. To investigate regulation of COX-2 and NGF by TGF-β, cul tured SSCT cells were stimulated with TGF-β and COX-2 and NGE mRNA and protein expression were evaluated us ing q-PCR and western blotting. Results: TGF-β-, NGF-, and COX-2-positive cells were ob served in SSCTs. Expression of COX-2 and NGF positively correlated with TGF-β mRNA expression in the SSCTs of CTS patients (COX-2, r = 0.629, p < 0.001; NGF, r = 0.521, p < 0.001). The expression of COX-2 and NGF mRNA and protein increased significantly in SSCT cells following ex ogenous treatment with TGF-β compared to vehicle-treated cells (p < 0.05). Conclusions: TGF-β may regulate COX-2 and NGF in the SSCTs of CTS patients.","PeriodicalId":244060,"journal":{"name":"International Archives of Orthopedic Surgery","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116764995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Analysis of Principal Stress Projection in Femur with Total Hip Arthroplasty using CT-image Based Finite Element Method 基于ct图像的有限元法分析全髋关节置换术后股骨主应力投影

International Archives of Orthopedic Surgery Pub Date : 2018-12-31 DOI: 10.23937/iaos-2017/1710003

Fukuoka Kosei, Todo Mitsugu

引用次数: 2