Life Science and Biotechnology最新文献

In Silico Primer Design for geographical detection of Apis florea using Cytochrome c oxidase subunit 1 (COX1) gene 细胞色素c氧化酶亚基1 (COX1)基因定位检测原料药的引物设计

Life Science and Biotechnology Pub Date : 2023-06-25 DOI: 10.19184/lsb.v1i1.40052

N. I. Shullia, K. Kuswati, A. Kurniawan, Hajar Syifa Fiarani

{"title":"In Silico Primer Design for geographical detection of Apis florea using Cytochrome c oxidase subunit 1 (COX1) gene","authors":"N. I. Shullia, K. Kuswati, A. Kurniawan, Hajar Syifa Fiarani","doi":"10.19184/lsb.v1i1.40052","DOIUrl":"https://doi.org/10.19184/lsb.v1i1.40052","url":null,"abstract":"The yellow dwarf honey bee, Apis florea are well distributed in South Asia to South East Asia. This species is expanded and introduce area from their original distribution. However, the distribution of this honey bee in Indonesia is unexplored. The cytochrome c oxidase subunit 1 (COX1) gene are success to detect original geographic of introduce A. florea found in Egypt. The A. florea specific primer of COX1 gene are needed to produce the molecular marker for geographical origin detection. Thus, this study aims to in silico design the COX1 gene primer of A. florea using Primer3 and Primer-BLAST. This study results the best candidate primer of Af6_COX1_F and Af6_COX1_R primers that start from 212 to 983 regarding the A. florea COX1 gene (NC_021401) and produce 772 bp of amplicon. The melting temperature of forward primer are 54. 63 and 55.58°C, GC content of 40 and 45%, GC clamp of 3 and 2 for forward and reverse primer, respectively. There are no secondary structure of those primer and 100% homolog with A. florea COX1 sequences, thus those primer is potentially as the geographical origin marker of A. florea.","PeriodicalId":243322,"journal":{"name":"Life Science and Biotechnology","volume":"21 8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116200677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening Ethanol-Producing Yeast Origin from Pineapple Honey Ananas comosus (L.) Merr var Quenn 菠萝蜂蜜产乙醇酵母菌的筛选mervar Quenn

Life Science and Biotechnology Pub Date : 2023-06-24 DOI: 10.19184/lsb.v1i1.39560

S. Sutoyo, S. Siswanto

{"title":"Screening Ethanol-Producing Yeast Origin from Pineapple Honey Ananas comosus (L.) Merr var Quenn","authors":"S. Sutoyo, S. Siswanto","doi":"10.19184/lsb.v1i1.39560","DOIUrl":"https://doi.org/10.19184/lsb.v1i1.39560","url":null,"abstract":"The availability of fossil-based fuels will gradually decrease while the amount required increases. This has prompted research to find alternative energy sources, including bioethanol. Bioethanol, as an alternative energy source, can be used as a transportation fuel better than fossil-based fuels because ethanol has a high octane number, laminar velocity and heat of vaporisation. Meanwhile, the level of carbon and hydrocarbon emissions is low so it can increase combustion efficiency in vehicles. In addition, the use of bioethanol as fuel is environmentally friendly and renewable. Ethanol can be produced through a fermentation process using ethanol-producing yeast. Pineapple honey is known for its high sugar and other nutrient content, so it is supposed that many types of yeast can grow well in it. This study aims to isolation and screening of bioethanol-producing yeast from Pineapple Honey. This study was carried out by yeast isolation method, primary yeast screening, secondary yeast screening, and characterisation of selected yeast isolates. Data on ethanol production by yeast isolates were analysed by ANOVA with α=0.05 using the R studio program. This primary isolation and screening phase found eight yeast isolates capable of fermenting glucose and producing acid and gas in the test medium. Test ethanol production in DPE medium with 18% glucose content on all isolates showed that all isolates could produce ethanol with a range of 4.20-10.35%. Isolates of IK4 showed the highest ethanol production ability (10.35%) (p<0.5%) in significant contrast to the other seven isolates. Based on macroscopic and microscopic morphological characterisations, IKM 4 isolates show similarities to the genus Saccharomyces \u0000Keywords: ethanol, pineapple, yeasts ","PeriodicalId":243322,"journal":{"name":"Life Science and Biotechnology","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122005189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The matK Mini-barcode as a Potential Molecular Identification Tool for Medicinal Orchids 作为药用兰科植物分子鉴定工具的matK迷你条形码

Life Science and Biotechnology Pub Date : 2023-06-24 DOI: 10.19184/lsb.v1i1.39851

Muhammad Suudi

{"title":"The matK Mini-barcode as a Potential Molecular Identification Tool for Medicinal Orchids","authors":"Muhammad Suudi","doi":"10.19184/lsb.v1i1.39851","DOIUrl":"https://doi.org/10.19184/lsb.v1i1.39851","url":null,"abstract":"matK is one of the universal loci which is commonly used as a barcode in the molecular identification of plants. Up to now, the problem of matK as a single locus is the use of the entire gene region as barcodes. The long amplicon size of matK raises several problems in DNA barcoding. The aim of this study is to uncover a small region of the matK gene as a potential mini-barcode region for alternative sites to get more efficient and effective results. In silico studies performed include the collection of matK sequence of medicinal orchid from GenBank, the amplicon size from matK primer sets that are commonly used in the Orchidaceae family, and PCR analysis. Data analysis was done by comparing the results of PCR amplification from several primer sets: matK 390F-1326R, 743F-R2, 712F-1154R and 712F-1216R. The results show that all primer pairs are able to amplify the DNA template isolated from Dendrobium purpureum. However, the shorter DNA band obtained from matK 743F-R2, 712F-1154R and 712F-1216R primers sets were visualized more thick than the longer amplicon band produced from matK primer 390F-1326R. This indicates that short matK sequence for DNA barcoding (mini-barcode) is more efficient in amplifying DNA templates compared to generally applied barcoding using full length sequences.","PeriodicalId":243322,"journal":{"name":"Life Science and Biotechnology","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129541342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Composition and Diversity of Fresh Water Fish in The Upstream of the Bedadung River, Jember District 江伯江上游淡水鱼的组成和多样性

Life Science and Biotechnology Pub Date : 2023-06-23 DOI: 10.19184/lsb.v1i1.38088

Rendy Setiawan Setiawan

引用次数: 0

Epiphytic ferns and orchids adaptation mechanism based on stomatal structure and chlorophyll content in Urban Forest of Jember University, Indonesia 基于气孔结构和叶绿素含量的城市森林附生蕨类和兰科植物适应机制

Life Science and Biotechnology Pub Date : 2023-06-23 DOI: 10.19184/lsb.v1i1.39678

Fuad bahrul Ulum, D. Setyati, M. Su’udi

引用次数: 0