M. E. Barnes, D. Bergmann, J. Larson, Ta-Kang Liu, Nathan Huysman
{"title":"Iodine Concentrations Prior to Initial Incubation Do Not Impact the Bacterial Communities of Landlocked Fall Chinook salmon Eggs at the Eyed Stage of Development","authors":"M. E. Barnes, D. Bergmann, J. Larson, Ta-Kang Liu, Nathan Huysman","doi":"10.15226/24754706/3/2/00127","DOIUrl":"https://doi.org/10.15226/24754706/3/2/00127","url":null,"abstract":"Using iodine solutions to disinfect newly-spawned salmonid eggs is well established. This study examined the bacterial communities of eggs from five landlocked Chinook salmon Oncorhynchus tshawytscha females subjected to 10-minute treatments’ of 100, 200, or 400 mg/l of active iodine prior to hatchery incubation. Bacterial sampling of the eggs occurred prior to disinfection, immediately after disinfection and 28-days later at the eyed stage of development. Hatchery incubation water was also sampled. The number of cultivable bacteria on the external membrane of freshly-spawned eggs ranged from 10 to over 66 colony-forming units. All of the iodine treatments significantly reduced the number of bacteria compared to pretreatment samples, but there were no significant differences among the treatments. Numbers of cultivable bacteria on eggs at the eyed stage after 28 days of incubation were approximately 105 times greater than those observed on the freshly-spawned eggs. There were no significant differences in egg survival among the iodine treatment groups. Acinetobacter dominated the egg surface prior to iodine disinfection, and Psychrobacter also was common. Flavobacterium was the most prevalent bacterial genus from eyed-eggs, representing over 43% of the sequences. Vibrio and Variovorax were also abundant. The bacterial flora of the hatchery water was dominated by Proteobacteria, especially Pseudomonas species, with Massilia, Zoogloea, Leptospirillum, and Rhodococcus also abundant. No effects of iodine treatment level on the abundance or genera of bacteria on eyed-eggs were evident.","PeriodicalId":242716,"journal":{"name":"International Journal of Marine Biology and Research","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115711491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. S. Reddy, Adiam Abraham, Berihu Afewerki, B. Tsegay, Henok Ghebremedhin, B. Teklehaimanot
{"title":"Extraction of Agar and Alginate from Marine Seaweeds in Red Sea Region","authors":"K. S. Reddy, Adiam Abraham, Berihu Afewerki, B. Tsegay, Henok Ghebremedhin, B. Teklehaimanot","doi":"10.15226/24754706/3/2/00126","DOIUrl":"https://doi.org/10.15226/24754706/3/2/00126","url":null,"abstract":"Water constitutes major portion of the earth’s crust approximately (75%), and it applies to all biological organisms, including sea weeds. Its extraction is useful to human beings directly and indirectly. The study was conducted in Red Sea area of Massawa beaches and extracted agar and alginate from two varieties of red algae (Gracilaria arcuate) and brown algae (Turbinaria decurrens). These extractnats were having many applications for human benefits and also industrial applications such as binding agent and agro-based industry. These were analysed for their phycocolloids extraction (specifically agar and alginate). 20.4% of agar was extracted from Gracilaria arcuata according to the procedure of Geratrudes (1990). Gracilaria arcuata were pre-treated with Na2CO3 followed by NaOH treatment and finally by extraction. The results were found to be encouraging in its powdered product has been obtained. Parallel to that 25.6% powdered Sodium alginate was extracted by Green process from the extraordinary species of brown algae, Turbinaria decurrens and as per chemical analysis, positive result was obtained. According to the data from Hernandez-Carmona et al, (2013), agar is the most expensive colloid in worlds market, at US$18 per kg, followed by alginates and carrageenan at US$12 and US$10.5 per kg, respectively. Hence, new methods for extraction is required to obtain high quality byproducts from sea weeds.","PeriodicalId":242716,"journal":{"name":"International Journal of Marine Biology and Research","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122912632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adel Alshawafi, M. Analla, Ebrahim Alwashali, Mariem Ahechti, M. Aksissou
{"title":"Impacts of Marine Waste, Ingestion of Microplastic in the Fish, Impact on Fishing Yield, M’diq, Morocco","authors":"Adel Alshawafi, M. Analla, Ebrahim Alwashali, Mariem Ahechti, M. Aksissou","doi":"10.15226/24754706/3/2/00125","DOIUrl":"https://doi.org/10.15226/24754706/3/2/00125","url":null,"abstract":"Marine pollution has always shown many impacts on the environment all around the world. In this study, two of the impacts of debris are evaluated on the northern coast of the Mediterranean Sea in Morocco. The evaluation of the ingestion phenomenon of micro plastic in the fish, demonstrated that 10% was the percentage of fish samples which ingested microplastic for both species with higher values for the benthic fish, Diplodus cervinus, for the pelagic fish, Auxis thazard. The other impact detected is the presence of marine litter; 86% is the percentage of occurrence of waste in the fishing nets, which means the accumulation of debris in the seabed. This growth of marine litter leads to increased risk for marine biology in general, and to the appearance of other negative effects; in particular the low economic yield of this maritime activity.","PeriodicalId":242716,"journal":{"name":"International Journal of Marine Biology and Research","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128426645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kamaruddin Eddiwan, E. fawani, Rizky Janatul Magwa
{"title":"Growth and N and P Absorption Capability of Pistia Stratiotes Cultured in the Inorganic Fertilizer Enriched Media","authors":"Kamaruddin Eddiwan, E. fawani, Rizky Janatul Magwa","doi":"10.15226/24754706/3/2/00124","DOIUrl":"https://doi.org/10.15226/24754706/3/2/00124","url":null,"abstract":"Pistia stratiotes are able to grow rapidly in culture medium given inorganic compounds. P. stratiotes cultured in a culture medium that has been given nitrates, phosphates and potassium (NPK) fertilizer. P. stratiotes cultured in a culture medium that has been given NPK fertilizer. A CRD with 5 concentrations of inorganic fertilizers tested, i.e. 0 (Co); 15 (T1); 20 (T2); 35 (T3); and 50 g / 10 L (T4). Observation of growth and decrease of the inorganic compound in culture medium was done 5 times, every 3 days. The results show that the best growth was identified in plants grown in T4. At the end of the experiment, 18.8 g BW with a daily growth rate was 17.9% of the baseline value of BW. On the 7th day of the experiment, the decrease was 0.187 mg / L (N) and 0.237 mg / L (P). So P. stratiotes were able to absorb relatively high N and P from water and use it for its growth.","PeriodicalId":242716,"journal":{"name":"International Journal of Marine Biology and Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129398487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Giorgio, Annaclaudia Montanino, M. G. Aquila, Beatrice Cocozziello, M. Guida
{"title":"A Comparison between Microscopic and Molecular Methods for Identification of Ostreopsis Species in Marine Samples","authors":"A. Giorgio, Annaclaudia Montanino, M. G. Aquila, Beatrice Cocozziello, M. Guida","doi":"10.15226/24754706/3/2/00123","DOIUrl":"https://doi.org/10.15226/24754706/3/2/00123","url":null,"abstract":"An assessment of O. ovata and O. cf. siamensis presence in some seawater samples and on macro algae was carried out in the Mediterranean Sea. The identification of taxa investigated was performed using traditional microscopic techniques and molecular tools, to evaluate the distribution area of these species too. Ostreopsis includes epiphytic/benthonic microalgae known for their production of toxic compounds that can cause biopoisoning. Microscopic analysis reveal that the presence of Ostreopsis spp. and O. ovata in macroalgal samples is greater than in water ones. In a greater number of samples, through molecular analysis the presence of investigated species has been detected, in comparison to the previous results of microscopic analysis. They also allowed identifying Ostreopsis spp and O. ovata species even in those samples in which microscopic analysis hasn’t detected any cells, probably because it’s beneath the detection limit of method. However for environmental monitoring the microscopic analysis have proved useful to detect the presence with high-precision of these species in a marine environment and to monitor their proliferation in time while molecular approach could be expensive, difficult to apply and inaccurate first of all because of the impossibility to distinguish live cells from dead cells.","PeriodicalId":242716,"journal":{"name":"International Journal of Marine Biology and Research","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127933496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}