Iranian journal of immunology : IJI最新文献

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Natural Killer Cell Subsets in Tumor Draining Lymph Nodes of Patients with Bladder Cancer and Their Clinical Implications. 膀胱癌患者肿瘤引流淋巴结中的自然杀伤细胞亚群及其临床意义
Iranian journal of immunology : IJI Pub Date : 2024-04-07 DOI: 10.22034/iji.2024.100799.2714
Ali Ariafar, Erfan Kohansal, Amirhassan Mousania, Z. Faghih
{"title":"Natural Killer Cell Subsets in Tumor Draining Lymph Nodes of Patients with Bladder Cancer and Their Clinical Implications.","authors":"Ali Ariafar, Erfan Kohansal, Amirhassan Mousania, Z. Faghih","doi":"10.22034/iji.2024.100799.2714","DOIUrl":"https://doi.org/10.22034/iji.2024.100799.2714","url":null,"abstract":"Background\u0000Natural killer (NK) cells are crucial innate components in anti-tumor immunity. However, the clinical impacts and their phenotypes in bladder cancer (BC) remain unclear.\u0000\u0000\u0000Objective\u0000To assess the clinical significance of NK cell subsets in tumor-draining lymph nodes of patients with BC.\u0000\u0000\u0000Methods\u0000In a cross-sectional study, pelvic lymph nodes were obtained from 49 untreated patients with BC. Mononuclear cells were isolated and immunophenotyped using CD3, CD56, CD16, CD27, and CD11b markers. NK cells were then classified based on their expression patterns of CD56/CD16 (conventional) and CD27/CD11b (new).\u0000\u0000\u0000Results\u0000On average, NK cells constituted 2.99±1.44% of the total lymphocytes in the draining lymph node of patients with BC. The CD56dim and regulatory NK subsets (CD27+CD11b+/-) were the predominant old and new NK, respectively. The NK cells significantly increased in patients with at least one involved node (LN+) compared with those with free nodes (LN-; p=0.022). Conversely, CD56dimCD16- subset significantly decreased in higher stages (p=0.032) and in tumors with muscle invasion (p=0.038). Significant variations were also observed in different T-stages (p<0.05). Regarding new classification, the frequency of CD11b+ regulatory NK cells was significantly lower in node-positive patients (p=0.025).\u0000\u0000\u0000Conclusion\u0000These findings emphasize the dynamic nature of NK cell subsets in bladder cancer and their potential relevance in disease progression and management, suggesting potential implications for therapeutic strategies targeting these specific subsets.","PeriodicalId":240487,"journal":{"name":"Iranian journal of immunology : IJI","volume":"57 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140733747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mycobacterium Vaccae Regulate γδT17 and γδTreg Cells in Mice Asthmatic Lung. 母牛分枝杆菌对哮喘小鼠肺中γδT17和γδTreg细胞的调节作用
IF 0.9
Iranian journal of immunology : IJI Pub Date : 2022-09-01 DOI: 10.22034/iji.2022.94460.2311
Yien Yao, Xiaoju Chen, Caicheng Qin, Jianlin Huang, Siyue Xu, Chaoqian Li
{"title":"Mycobacterium Vaccae Regulate γδT17 and γδTreg Cells in Mice Asthmatic Lung.","authors":"Yien Yao,&nbsp;Xiaoju Chen,&nbsp;Caicheng Qin,&nbsp;Jianlin Huang,&nbsp;Siyue Xu,&nbsp;Chaoqian Li","doi":"10.22034/iji.2022.94460.2311","DOIUrl":"https://doi.org/10.22034/iji.2022.94460.2311","url":null,"abstract":"<p><p>Background Dysregulation of the balance between different T cell populations is believed to be an important basis for asthma.Objective To observe the changes in γδT subtypes in transgenic asthmatic mice after aerosol inhalation of Mycobacterium vaccae, and to further investigate the mechanism of M. vaccae in asthmatic mice and its relationship with γδT cells.Methods TCR-β-/- mice were exposed to atomized normal saline or M. vaccae for 5 days and the γδT cells from the lung tissues were isolated. Changes in γδT17 and γδTreg populations were detected. Asthma was induced in BALB/c mice using ovalbumin, which was then transplanted with control or M. vaccae-primed γδT cells. First we analyzed the content of γδT cells that secrete IL-17 (IL-17 γδT cells) and Foxp3+ γδT cells in lung tissues and then measured the content of IL-17 in the bronchoalveolar lavage fluid (BALF) by ELISA.Results Exposure to M. vaccae increased and decreased the relative proportions of Foxp3+ γδT cells and IL-17+ γδT cells, respectively, thereby decreasing airway reactivity and inflammation levels in asthmatic mice, and significantly decreasing IL-17 levels in BALF. Furthermore, mice treated with these primed T cells showed a decrease in IL-17+ γδT cells, and a concomitant increase in Foxp3+ γδT cells in their lung tissues. Furthermore, adoptive transfer of M. vaccae-primed γδT cells decreased GATA3 and NICD and increased T-bet in lung.Conclusions The M. vaccae-primed γδT cells alleviated the symptoms of asthma by reversing Th2 polarization in the lungs and inhibiting the Notch/GATA3 pathway.</p>","PeriodicalId":240487,"journal":{"name":"Iranian journal of immunology : IJI","volume":" ","pages":"243-254"},"PeriodicalIF":0.9,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40394423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization of an Efficient Cell Culture Hepatitis B Infection System for Assessment of Hepatitis B Virus Neutralizing Monoclonal Antibodies. 乙型肝炎病毒中和性单克隆抗体高效细胞培养感染系统的优化
IF 0.9
Iranian journal of immunology : IJI Pub Date : 2022-09-01 DOI: 10.22034/iji.2022.94266.2298
Hamzeh Sarvnaz, Sahar Asadi-Asadabad, Mohammad Mehdi Amiri, Mojgan Ghaedi, Ulrike Protzer, Mahmood Jeddi-Tehrani, Forough Golsaz-Shirazi, Fazel Shokri
{"title":"Optimization of an Efficient Cell Culture Hepatitis B Infection System for Assessment of Hepatitis B Virus Neutralizing Monoclonal Antibodies.","authors":"Hamzeh Sarvnaz,&nbsp;Sahar Asadi-Asadabad,&nbsp;Mohammad Mehdi Amiri,&nbsp;Mojgan Ghaedi,&nbsp;Ulrike Protzer,&nbsp;Mahmood Jeddi-Tehrani,&nbsp;Forough Golsaz-Shirazi,&nbsp;Fazel Shokri","doi":"10.22034/iji.2022.94266.2298","DOIUrl":"https://doi.org/10.22034/iji.2022.94266.2298","url":null,"abstract":"<p><strong>Background: </strong>Human polyclonal plasma-derived hepatitis B immunoglobulin (HBIG) is currently used for immunoprophylaxis of HBV infection. The development of virus-neutralizing monoclonal antibodies (MAbs) requires the use of optimized cell culture systems supporting HBV infection.</p><p><strong>Objective: </strong>This study aims to optimize the hepatitis B virus infectivity of NTCP-reconstituted HepG2 (HepG2-NTCP) cells to establish an efficient system to evaluate the HBV-neutralizing effect of anti-HBs MAbs.</p><p><strong>Methods: </strong>Serum-derived HBV (sHBV) and cell culture-derived HBV (ccHBV) were simultaneously used for the optimization of HBV infection in HepG2-NTCP cells by applying different modifications.</p><p><strong>Results: </strong>Our results for the first time showed that in addition to human serum, monkey serum could significantly improve ccHBV infection, while fetal and adult bovine serum as well as duck and sheep serum did not have a promotive effect. In addition, sHBV and ccHBV infectivity are largely similar except that adding 5% of PEG, which is commonly used to improve in vitro infection of ccHBV, significantly reduced sHBV infection. We showed that a combination of spinoculation, trypsinization, and also adding human or monkey serum to HBV inoculum could significantly improve the permissivity of HepG2-NTCP cells to HBV infection compared with individual strategies. All anti-HBs MAbs were able to successfully neutralize both ccHBV and sHBV infection in our optimized in vitro system.</p><p><strong>Conclusion: </strong>Our study suggests different strategies for improving ccHBV and sHBV infection in HepG2-NTCP cells. This cell culture-based system allows assessment of HBV neutralizing MAbs and may also prove to be valuable for the analysis of other HBV neutralizing therapeutics.</p>","PeriodicalId":240487,"journal":{"name":"Iranian journal of immunology : IJI","volume":" ","pages":"278-298"},"PeriodicalIF":0.9,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40394426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rosuvastatin Intervention Decreased the Frequencies of the TIM-3+ Population of NK Cells and NKT Cells among Patients with Chronic Hepatitis B. 瑞舒伐他汀干预降低慢性乙型肝炎患者NK细胞和NKT细胞TIM-3+群的频率
IF 0.9
Iranian journal of immunology : IJI Pub Date : 2022-09-01 DOI: 10.22034/iji.2022.93726.2250
Alireza Norouzi, Shohreh Taziki, Ali Najafipasandi, Saeed Mohammadi, Gholamreza Roshandel
{"title":"Rosuvastatin Intervention Decreased the Frequencies of the TIM-3+ Population of NK Cells and NKT Cells among Patients with Chronic Hepatitis B.","authors":"Alireza Norouzi,&nbsp;Shohreh Taziki,&nbsp;Ali Najafipasandi,&nbsp;Saeed Mohammadi,&nbsp;Gholamreza Roshandel","doi":"10.22034/iji.2022.93726.2250","DOIUrl":"https://doi.org/10.22034/iji.2022.93726.2250","url":null,"abstract":"<p><strong>Background: </strong>Natural killer (NK) cells are dichotomously involved in chronic hepatitis B (CHB) infection as principal members of innate immunity. An effective treatment should enhance the antiviral potentials of NK cells and not their immunomodulatory roles. TIM-3 (T-cell immunoglobulin and mucin-containing domain) is a molecule with an essential role in controlling immune tolerance. TIM-3 demonstrated the highest expression among NK cells of patients with chronic liver disorders. Statins have been reported to attenuate the levels of TIM-3 on NK cells.</p><p><strong>Objectives: </strong>To investigate the frequencies of NK cells, NKT cells, and TIM-3+ population in patients with CHB upon rosuvastatin (RSV) intervention.</p><p><strong>Methods: </strong>Thirty confirmed patients with CHB were randomly assigned into two groups of 15 (receiving 20 mg of RSV or placebo per day) for 12 weeks. We evaluated the percentages of TIM-3+ cells by staining the peripheral blood mononuclear cells (PBMCs) with CD3, CD16, and CD56 markers using flow cytometry.</p><p><strong>Results: </strong>Our findings indicated that RSV administration could increase CD3- CD56+ NK cells (P>0.05) and CD3+ CD16+ CD56+ NKT cells (P<0.05). RSV intervention could reduce the percentages of TIM-3+ cells among NK cells (P<0.01) and NKT cells (P> 0.05) of patients with CHB compared with the placebo group.</p><p><strong>Conclusions: </strong>The increased population of NK and NKT cells and the effective reduction of TIM-3+ cells among patients with CHB delineated that rosuvastatin could be proposed as an appropriate modulator of innate immune response (regarding NK and NKT cells) in favor of enhancing their antiviral activities.</p>","PeriodicalId":240487,"journal":{"name":"Iranian journal of immunology : IJI","volume":" ","pages":"255-262"},"PeriodicalIF":0.9,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40394424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Labor Could Increase Systemic Inflammation and Cause or Deteriorate Cytokine Storm in COVID-19. 分娩可能增加全身炎症,导致或恶化COVID-19的细胞因子风暴。
IF 0.9
Iranian journal of immunology : IJI Pub Date : 2022-09-01 DOI: 10.22034/iji.2022.93951.2267
Amir Hossein Norooznezhad, Alireza A Shamshirsaz, Sedigheh Hantoushzadeh
{"title":"Labor Could Increase Systemic Inflammation and Cause or Deteriorate Cytokine Storm in COVID-19.","authors":"Amir Hossein Norooznezhad,&nbsp;Alireza A Shamshirsaz,&nbsp;Sedigheh Hantoushzadeh","doi":"10.22034/iji.2022.93951.2267","DOIUrl":"https://doi.org/10.22034/iji.2022.93951.2267","url":null,"abstract":"<p><p>Pregnant women with coronavirus disease 2019 (COVID-19) have a higher risk of morbidity and mortality compared with the general population. Possible pathways are: I) in patients with COVID-19, cytokine storm defined as the excess release of pro-inflammatory cytokines such as interleukin 1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α) has been associated with morbidities and an even higher rate of mortality. II) Labor, despite being a term/preterm, has an inflammatory nature, although, inflammation is more prominent in preterm delivery. During labor, different pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α are involved which as mentioned, all are crucial role players in the cytokine storm. III) Tissue injury, and during labor, (especially cesarean section) is shown to cause inflammation via pro-inflammatory cytokines release including those involved in the cytokine storm through the activation of nuclear factor κB (NFκB). IV) post-partum hemorrhage with a notable amount of blood loss which can cause significant hypoxemia. In this condition, hypoxia-inducible factor 1α which has a cross-talk with NFκB, leads to the expression of IL-1β, IL-6, and TNF-α as both angiogenic and pro-inflammatory factors. Considering all the mentioned issues and pathways, we suggest that clinicians be careful about the escalation of the inflammatory status in their pregnant COVID-19 patients during/following labor.</p>","PeriodicalId":240487,"journal":{"name":"Iranian journal of immunology : IJI","volume":" ","pages":"330-336"},"PeriodicalIF":0.9,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40394429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The Proportion of Vδ1T Cells in Peripheral Blood Correlated with Prognosis of Sepsis. 外周血Vδ1T细胞比例与脓毒症预后的关系
IF 0.9
Iranian journal of immunology : IJI Pub Date : 2022-09-01 DOI: 10.22034/iji.2022.89256.1934
Fawei Yuan, Huan Yin, Juan Tan, Kun Zheng, Xiping Mei, Lixue Yuan
{"title":"The Proportion of Vδ1T Cells in Peripheral Blood Correlated with Prognosis of Sepsis.","authors":"Fawei Yuan,&nbsp;Huan Yin,&nbsp;Juan Tan,&nbsp;Kun Zheng,&nbsp;Xiping Mei,&nbsp;Lixue Yuan","doi":"10.22034/iji.2022.89256.1934","DOIUrl":"https://doi.org/10.22034/iji.2022.89256.1934","url":null,"abstract":"<p><strong>Background: </strong>Sepsis is a serious condition with a high mortality rate, and septic patients often have organ dysfunction, low tissue perfusion and hypoxia, lactic acidosis, oliguria, or functional brain changes.</p><p><strong>Objective: </strong>To observe the number and the function of Vδ1T cells in peripheral blood of septic patients, to analyze the clinical significance of detecting Vδ1T cells, and to clarify the correlation of their presence with the prognosis of sepsis.</p><p><strong>Methods: </strong>The basic data of the septic patients were recorded at admission. The immunosuppressive function-related molecules on the surface of Vδ1T cells were detected, and the immunosuppressive function of Vδ1T cells was also evaluated.</p><p><strong>Results: </strong>Compared with the healthy controls, the proportion of Vδ1T cells in the blood of septic patients significantly decreased (P<0.01). The proportion of Vδ1T cells in septic patients correlated with the patients' condition (P<0.05). The expression of glucocorticoid-induced tumor necrosis factor receptor (GITR), cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), and T-cell immunoglobulin and mucin domain-containing protein 3 (TIM-3) on the surface of Vδ1T cells in the blood of septic patients significantly increased (P<0.01). The increase of Vδ1T cells in septic patients had inhibitory effects on T cell proliferation and interferon (IFN)-γ secretion. These findings implied that the immunosuppression of Vδ1Tcells in the peripheral blood of septic patients was significantly higher than that of the healthy controls (P<0.01).</p><p><strong>Conclusion: </strong>Changes in Vδ1T cells in septic patients were closely related to the patient's condition and prognosis.</p>","PeriodicalId":240487,"journal":{"name":"Iranian journal of immunology : IJI","volume":" ","pages":"232-242"},"PeriodicalIF":0.9,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40393012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Chemokine MIG is Associated with an Increased Risk of COVID-19 Mortality in Mexican Patients. 趋化因子MIG与墨西哥患者COVID-19死亡风险增加有关
IF 0.9
Iranian journal of immunology : IJI Pub Date : 2022-09-01 DOI: 10.22034/iji.2022.92641.2162
Luis Antonio Ochoa-Ramirez, Rosalio Ramos-Payan, German Reynaldo Jimenez-Gastelum, Jose Rodriguez-Millan, Maribel Aguilar-Medina, Juan Jose Rios-Tostado, Alfredo Ayala-Ham, Mercedez Bermudez, Juan Fidel Osuna-Ramos, Vicente Olimon-Andalon, Jesús Salvador Velarde-Félix
{"title":"The Chemokine MIG is Associated with an Increased Risk of COVID-19 Mortality in Mexican Patients.","authors":"Luis Antonio Ochoa-Ramirez,&nbsp;Rosalio Ramos-Payan,&nbsp;German Reynaldo Jimenez-Gastelum,&nbsp;Jose Rodriguez-Millan,&nbsp;Maribel Aguilar-Medina,&nbsp;Juan Jose Rios-Tostado,&nbsp;Alfredo Ayala-Ham,&nbsp;Mercedez Bermudez,&nbsp;Juan Fidel Osuna-Ramos,&nbsp;Vicente Olimon-Andalon,&nbsp;Jesús Salvador Velarde-Félix","doi":"10.22034/iji.2022.92641.2162","DOIUrl":"https://doi.org/10.22034/iji.2022.92641.2162","url":null,"abstract":"<p><strong>Background: </strong>Coronavirus disease 2019 (COVID-19) is an emergent viral disease in which the host inflammatory response modulates the clinical outcome. Severe outcomes are associated with an exacerbation of inflammation in which chemokines play an important role as the attractants of immune cells to the tissues.</p><p><strong>Objective: </strong>To evaluate the relationship of the chemokines IL-8, RANTES, MIG, MCP-1, and IP-10 with COVID-19 severity and outcomes in Mexican patients.</p><p><strong>Methods: </strong>We analyzed the serum levels of IL-8, RANTES, MIG, MCP-1 and IP-10 in 148 COVID-19 hospitalized patients classified as mild (n=20), severe (n=61), and critical (n=67), as well as in healthy individuals (n=10), by flow cytometry bead array assay.</p><p><strong>Results: </strong>Chemokine levels were higher in patients than in the healthy individuals, but only MIG, MCP-1, and IP-10 increased according to the disease severity, showing the highest levels in the critical group. MIG, MCP-1, and IP-10 levels were also higher in COVID-19 patients with comorbidities such as renal disease, type 2 diabetes, and hypertension. Moreover, elevated MIG levels seem to be related to organic failure/shock, and an increased risk of death.</p><p><strong>Conclusions: </strong>Our results suggest that the increased levels of MCP-1, IP-10, and especially MIG might be useful in predicting severe COVID-19 outcomes and could be promising therapeutic targets.</p>","PeriodicalId":240487,"journal":{"name":"Iranian journal of immunology : IJI","volume":" ","pages":"311-320"},"PeriodicalIF":0.9,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40394428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Effects of Lipopolysaccharide from Porphyromonas gingivalis and Escherichia coli on Gene Expression Levels of Toll-like Receptors and Inflammatory Cytokines in Human Dental Pulp Stem Cells. 牙龈卟啉单胞菌和大肠杆菌脂多糖对人牙髓干细胞toll样受体和炎症因子基因表达水平的影响
IF 0.9
Iranian journal of immunology : IJI Pub Date : 2022-09-01 DOI: 10.22034/iji.2022.92223.2136
Hanieh Mojtahedi, Nikoo Hossein-Khannazer, Seyed Mahmoud Hashemi, Mina Masoudnia, Monireh Askarzadeh, Arash Khojasteh, Mandana Sattari
{"title":"Effects of Lipopolysaccharide from Porphyromonas gingivalis and Escherichia coli on Gene Expression Levels of Toll-like Receptors and Inflammatory Cytokines in Human Dental Pulp Stem Cells.","authors":"Hanieh Mojtahedi,&nbsp;Nikoo Hossein-Khannazer,&nbsp;Seyed Mahmoud Hashemi,&nbsp;Mina Masoudnia,&nbsp;Monireh Askarzadeh,&nbsp;Arash Khojasteh,&nbsp;Mandana Sattari","doi":"10.22034/iji.2022.92223.2136","DOIUrl":"https://doi.org/10.22034/iji.2022.92223.2136","url":null,"abstract":"<p><strong>Background: </strong>Periodontal diseases originate from a group of oral inflammatory infections initiated by oral pathogens. Among these pathogens, Gram-negative bacteria such as p. gingivalis play a major role in chronic periodontitis. P. gingivalis harbours lipopolysaccharide (LPS) which enables it to attach to TLR2.</p><p><strong>Objectives: </strong>Evaluating the effects of P. gingivalis and E. coli LPS on the gene expression of TLRs and inflammatory cytokines in human dental pulp stem cells (hDPSCs).</p><p><strong>Methods: </strong>We evaluated the expression level of TLR2, TLR4, IL-6, IL-10, and 1L-18 in hDPSCs treated with 1μg/mL of P. gingivalis lipopolysaccharide and E. coli LPS at three different exposure times using Real-time RT-PCR.</p><p><strong>Result: </strong>The test group treated with P. gingivalis LPS showed a high level of TLR4 expression in 24 hours exposure period and the lowest expression in 48 hours of exposure time. In the case of IL-10, the lowest expression was in the 24 hours exposure period. Although in the E.coli LPS treated group, IL-10 showed the highest expression in 24 and lowest in 48 hours exposure period. Moreover, IL-18 in P. gingivalis LPS treated group showed a significant difference between 6, 24, and 48-time periods of exposure, but not in the E. coli LPS treated group.</p><p><strong>Conclusion: </strong>Both types of LPS stimulate inflammation through TLR4 expression. P. gingivalis LPS performs more potentially than E. coli in terms of stimulating inflammation at the first 24 hours of exposure. Nevertheless, our study confirmed that increasing P. gingivalis and/or the E.coli LPS exposure time, despite acting as an inflammatory stimulator, apparently showed anti-inflammatory properties.</p>","PeriodicalId":240487,"journal":{"name":"Iranian journal of immunology : IJI","volume":" ","pages":"299-310"},"PeriodicalIF":0.9,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40394427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Letter to the Editor Regarding "An Overview on Serology and Molecular Tests for COVID-19: An Important Challenge of the Current Century (doi: 10.22034/iji.2021.88660.1894.)". 关于“COVID-19血清学和分子检测综述:本世纪的重要挑战”的致编辑信(doi: 10.22034/iji.2021.88660.1894.)。
IF 0.9
Iranian journal of immunology : IJI Pub Date : 2022-09-01 DOI: 10.22034/iji.2022.91791.2107
Milad Zandi, Emad Behboudi, Mohammad Reza Shojaei, Saber Soltani, Hassan Karami
{"title":"Letter to the Editor Regarding \"An Overview on Serology and Molecular Tests for COVID-19: An Important Challenge of the Current Century (doi: 10.22034/iji.2021.88660.1894.)\".","authors":"Milad Zandi,&nbsp;Emad Behboudi,&nbsp;Mohammad Reza Shojaei,&nbsp;Saber Soltani,&nbsp;Hassan Karami","doi":"10.22034/iji.2022.91791.2107","DOIUrl":"https://doi.org/10.22034/iji.2022.91791.2107","url":null,"abstract":"<p><p>Recently in a review article by Mansourabadi et al. published in the Iranian Journal of Immunology, the authors described the serological and molecular tests for COVID-19 (1). The mentioned review considered helicase (Hel) as a structural protein of SARS-CoV-2 (1). However, based on evidence, the genome of novel coronavirus is approximately 30kb in length and encodes only four structural proteins, including spike (S), envelope (E), membrane (M), and nucleoprotein (N) (2, 3), although helicase (NSP13) as a nonstructural protein such as RNA-dependent RNA polymerases (NSP12) encoded by the ORF region and is involved in the replication of the virus (3).In addition, authors reported that hemagglutinin esterase could be used as a favorite target for SARS-CoV-2 Real-time PCR (1); however, scientific evidence shows that SARS-CoV-2 as a betacoronavirus lineage B like SARS-CoV lacks hemagglutinin esterase (4-6); thus this protein cannot be a target for detection of SARS-CoV-2.</p>","PeriodicalId":240487,"journal":{"name":"Iranian journal of immunology : IJI","volume":" ","pages":"337"},"PeriodicalIF":0.9,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40394430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Frequency of CD39+, LAG3+, and CTLA4+ Regulatory T Cells in Two Different Immunosuppressive Protocols in Renal Allograft Recipients (Sirolimus vs Mycophenolate mofetil): A Cohort Report. 两种不同免疫抑制方案(西罗莫司vs霉酚酸酯)肾移植受者中CD39+、LAG3+和CTLA4+调节性T细胞的频率:一项队列报告
IF 0.9
Iranian journal of immunology : IJI Pub Date : 2022-09-01 DOI: 10.22034/iji.2022.92623.2161
Yaghoub Mollaei-Kandelous, Pedram Ahmadpoor, Mohsen Nafar, Mohammad Reza Khatami, Samad Farashi Bonab, Nader Tajik, Mahdi Shekarabi, Aliakbar Amirzargar
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