Neural Imaging and Sensing 2019最新文献

Neural Imaging and Sensing 2019 Pub Date : 2019-04-29 DOI: 10.1117/12.2531543

引用次数: 0

Interferometric near-infrared spectroscopy (iNIRS) of human tissues in the presence of motion (Conference Presentation) 运动状态下人体组织的干涉近红外光谱(iNIRS)(会议报告)

Neural Imaging and Sensing 2019 Pub Date : 2019-03-04 DOI: 10.1117/12.2507077

Oybek Kholiqov, Wenjun Zhou, Tingwei Zhang, V. Srinivasan

{"title":"Interferometric near-infrared spectroscopy (iNIRS) of human tissues in the presence of motion (Conference Presentation)","authors":"Oybek Kholiqov, Wenjun Zhou, Tingwei Zhang, V. Srinivasan","doi":"10.1117/12.2507077","DOIUrl":"https://doi.org/10.1117/12.2507077","url":null,"abstract":"Interferometric near-infrared spectroscopy (iNIRS) is a time-of-flight- (TOF-) resolved sensing method for direct and simultaneous quantification of tissue optical properties (absorption and reduced scattering) and dynamics (blood flow index) in vivo with a single modality. The technique has previously been validated in Intralipid phantoms, and applied to continuously and non-invasively monitor optical properties and blood flow index in the brains of head-fixed, anesthetized mice. A demonstration of robust iNIRS measurements in human tissues with motion would support the viability of iNIRS for clinical applications. Here, we perform non-contact iNIRS in human tissues. We show that phase drift caused by involuntary motion during acquisition significantly distorts the optical field autocorrelation, particularly at early TOFs. To solve this issue, we present a novel numerical phase drift correction method to isolate field dynamics due to just red blood cell motion within the sample. Upon correction, TOF-resolved autocorrelations exhibit exponential decay behavior, whether acquired from Intralipid, the human forearm, or the human forehead. We confirm the link between bulk motion artifacts and phase drift by simultaneous, co-registered iNIRS and Optical Coherence Tomography measurements. By applying conventional, time-resolved diffusion theory and diffusing wave spectroscopy theory, we quantify optical properties and time-of-flight-resolved dynamics in Intralipid, the human forearm, and the human brain. Finally, we explore strategies for increased photon collection through parallelization of iNIRS, to probe greater depths in the human brain. This work conclusively shows that diffuse optical measurements of field dynamics are possible, even in the presence of motion artifacts.","PeriodicalId":239188,"journal":{"name":"Neural Imaging and Sensing 2019","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121425931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

All-optical readout and stimulation of cortical activity during optogenetically-triggered motor task in awake mice (Conference Presentation) 清醒小鼠光遗传触发运动任务期间的全光读出和皮层活动刺激(会议报告)

Neural Imaging and Sensing 2019 Pub Date : 2019-03-04 DOI: 10.1117/12.2508322

F. Resta, E. Montagni, Emilia Conti, G. Vito, A. Mascaro, F. Pavone

{"title":"All-optical readout and stimulation of cortical activity during optogenetically-triggered motor task in awake mice (Conference Presentation)","authors":"F. Resta, E. Montagni, Emilia Conti, G. Vito, A. Mascaro, F. Pavone","doi":"10.1117/12.2508322","DOIUrl":"https://doi.org/10.1117/12.2508322","url":null,"abstract":"In this study, we developed a wide-field all-optical system based on a red-shifted GECI (RCaMP1a) combined with channelrhodopsin II (ChR2) for simultaneous stimulation and readout of neuronal activity. Our results show that RCaMP1a transfection in primary motor cortex extends all over the cortical motor areas. The RCaMP1a and ChR2 reporter expression largely overlap, thus allowing the stimulation and readout from the same functional areas. Furthermore, we characterized the functional response by performing single pulse optogenetic stimulation and we observed that evoked calcium signals increase at increasing laser power. In order to study the cortical activation underlying a specific motor behavior, we performed optogenetic-stimulation of the Rostral Forelimb Area (RFA) with a train of lasers pulses. We observed that during 1s of 16 Hz train stimulus the animals suddenly start grasping with the contralateral forelimb. Cortical dynamics recorded during the optogenetically-triggered motor task show correlated activity between the RFA and the nearby motor areas. The all-optical system optimization and the possibility to link the neuronal population activity with the animal behavior would be a key point in understanding the network activity underlying a specific behavior.","PeriodicalId":239188,"journal":{"name":"Neural Imaging and Sensing 2019","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122249827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging trends in neural imaging and sensing (Conference Presentation) 神经成像和传感的新趋势(会议报告)

Neural Imaging and Sensing 2019 Pub Date : 2019-03-04 DOI: 10.1117/12.2509573

H. Shi, Zhen Wang

引用次数: 0

Simultaneous optical imaging and manipulation of the whole-brain neuronal activities in behaving zebrafish larvae (Conference Presentation) 斑马鱼幼虫全脑神经元活动的同步光学成像和操作(会议报告)

Neural Imaging and Sensing 2019 Pub Date : 2019-03-04 DOI: 10.1117/12.2508187

Zhenfei Jiao, Chun-feng Shang, Yu-fan Wang, Zhe Yang, Chen Yang, Funing Li, Jin-Ze Xie, Jiulin Du, L. Fu

{"title":"Simultaneous optical imaging and manipulation of the whole-brain neuronal activities in behaving zebrafish larvae (Conference Presentation)","authors":"Zhenfei Jiao, Chun-feng Shang, Yu-fan Wang, Zhe Yang, Chen Yang, Funing Li, Jin-Ze Xie, Jiulin Du, L. Fu","doi":"10.1117/12.2508187","DOIUrl":"https://doi.org/10.1117/12.2508187","url":null,"abstract":"In neuroscience, it’s one of the central goals to decipher neuronal ensembles’ function in the neural circuits. Recently, the progress of probes for optogenetic actuators and calcium indicators afford the probability for simultaneous optical manipulation and imaging of neuronal ensembles. Although with point-scanning imaging and localized optical stimulation, previous work had achieved all-optical interrogation of neural circuits in the local brain areas of mice and zebrafish larvae, no attempt was made to optical interrogate neuronal ensembles’ function across the whole-brain. Here, we combined the fast volumetric imaging technique, light-sheet microscopy which was an order of magnitude faster than the point-scanning method, with digital laser beam shaping device, digital micromirror device (DMD), to learn about neuronal activities across the whole brain of behaving larval zebrafish. Using the spectrally separated calcium indicator, GCaMP6f, and activity actuator, ChrimsonR, we can simultaneously image and manipulate neuronal ensembles with minimal spectral cross-talk. Besides, to monitor the behavior of zebrafish larvae, a high-speed camera was introduced into the system. To demonstrate the system’s function, arbitrarily selected neurons was stimulated during the whole-brain neuronal activities’ imaging, which effectively activated the synaptic connections and brain-wide downstream neural circuits and evoked stereotyped behaviors. These results demonstrate the system’s ability in studying the function of brain-wide neuronal ensembles in small animals.","PeriodicalId":239188,"journal":{"name":"Neural Imaging and Sensing 2019","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127007314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-color volumetric imaging of neural activity of cortical columns in vivo (Conference Presentation) 活体皮质柱神经活动的双色体积成像(会议报告)

Neural Imaging and Sensing 2019 Pub Date : 2019-03-04 DOI: 10.1117/12.2513315

Shuting Han, Weijian Yang, R. Yuste

引用次数: 0