Global Journal of Gastroenterology & Hepatology最新文献

筛选
英文 中文
Detecting GPC3-Expressing Hepatocellular Carcinoma with L5 Peptide-Guided Pretargeting Approach: An In Vitro MRI Experiment 用L5肽引导的预靶向方法检测表达gpc3的肝细胞癌:体外MRI实验
Global Journal of Gastroenterology & Hepatology Pub Date : 2018-08-01 DOI: 10.12970/2308-6483.2018.06.02
Weiyue Li, Xiang Xiao, Yikai Xu, Lichao Ma, Xiaodan Li, Liuji Guo, Chenggong Yan, Yuankui Wu
{"title":"Detecting GPC3-Expressing Hepatocellular Carcinoma with L5 Peptide-Guided Pretargeting Approach: An In Vitro MRI Experiment","authors":"Weiyue Li, Xiang Xiao, Yikai Xu, Lichao Ma, Xiaodan Li, Liuji Guo, Chenggong Yan, Yuankui Wu","doi":"10.12970/2308-6483.2018.06.02","DOIUrl":"https://doi.org/10.12970/2308-6483.2018.06.02","url":null,"abstract":"Background and Aim : Glypican-3 (GPC3) is a novel molecular target for hepatocellular carcinoma (HCC). This study investigated the potential of an L5 peptide-guided pretargeting approach to identify GPC3-expressing HCC cells using ultra-small super-paramagnetic iron oxide (USPIO) as the MRI probe. Methods : Immunofluorescence with carboxyfluorescein (FAM)-labeled L5 peptide was performed in HepG 2 and HL-7702 cells. Polyethylene glycol-modified ultrasmall superparamagnetic iron oxide (PEG-USPIO) and its conjugates with streptavidin (SA-PEG-USPIO) were synthesized, and hydrodynamic diameters, zeta potential, T 2 relaxivity, and cytotoxicity were measured. MR T 2 -weighted imaging of HepG 2 was performed to observe signal changes in the pretargeting group, which was first incubated with biotinylated L5 peptide and then with SA-PEG-USPIO. Prussian blue staining of cells was used to assess iron deposition. Results : Immunofluorescence assays showed high specificity of L5 peptide for GPC3. SA-PEG-USPIO nanoparticles had ≈36 nm hydrodynamic diameter, low toxicity, negative charge and high T2 relaxivity. MR imaging revealed that a significant negative enhancement was only observed in HepG 2 cells from the pretargeting group, which also showed significant iron deposition with Prussian blue staining. Conclusion : MR imaging with USPIO as the probe has potential to identify GPC3-expressing HCC through L5 peptide-guided pretargeting approach.","PeriodicalId":233773,"journal":{"name":"Global Journal of Gastroenterology & Hepatology","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130644099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信