MatSciRN: Stem Cell Bioengineering (Topic)最新文献

筛选

英文中文

Loss of XIST Impairs Human Mammary Stem Cell Differentiation and Increases Tumorigenicity Through Enhancer and Mediator Complex Hyperactivation XIST的缺失损害了人乳腺干细胞的分化，并通过增强子和中介复合物的过度激活增加致瘤性

MatSciRN: Stem Cell Bioengineering (Topic) Pub Date : 2021-03-22 DOI: 10.2139/ssrn.3809998

Laia Richart, Mary-Loup Picod, M. Wassef, M. Macario, Setareh Aflaki, Marion A. Salvador, Julien Wicinski, V. Chevrier, S. Le Cam, Hanya A. Kamhawi, R. Castellano, Géraldine Guasch, E. Charafe-Jauffret, E. Heard, R. Margueron, C. Ginestier

{"title":"Loss of XIST Impairs Human Mammary Stem Cell Differentiation and Increases Tumorigenicity Through Enhancer and Mediator Complex Hyperactivation","authors":"Laia Richart, Mary-Loup Picod, M. Wassef, M. Macario, Setareh Aflaki, Marion A. Salvador, Julien Wicinski, V. Chevrier, S. Le Cam, Hanya A. Kamhawi, R. Castellano, Géraldine Guasch, E. Charafe-Jauffret, E. Heard, R. Margueron, C. Ginestier","doi":"10.2139/ssrn.3809998","DOIUrl":"https://doi.org/10.2139/ssrn.3809998","url":null,"abstract":"X-chromosome inactivation (XCI) is triggered by up-regulation of XIST, which coats the chromosome in cis and promotes recruitment of chromatin modifiers that transform the X into a silent heterochromatic domain. Whether XIST plays a role beyond initiation of XCI is unclear. Here, we demonstrate that XIST loss impairs differentiation of human mammary stem cells (MaSC) and, upon oncogenic transformation, promotes emergence of highly metastatic carcinomas. On the Xi, XIST-deficient MaSC display epigenetic erosion and reactivation of genes overlapping Polycomb domains. Among reactivated loci we identify MED14 , a critical backbone of Mediator, and show that MED14 overdosage is sufficient to explain how defective XCI maintenance can stabilize MaSC enhancer landscape and transcriptional program, making differentiation less favorable. We conclude that XIST is a gatekeeper of mammary epithelium homeostasis, thus unveiling a new paradigm in the control of somatic cell identity with potential consequences in our understanding of gender-specific malignancies.","PeriodicalId":228505,"journal":{"name":"MatSciRN: Stem Cell Bioengineering (Topic)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114364623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Optogenetics: The Key to Deciphering and Curing Neurological Diseases 光遗传学:破解和治疗神经系统疾病的关键

MatSciRN: Stem Cell Bioengineering (Topic) Pub Date : 2020-12-02 DOI: 10.15354/si.20.re081

Fuzhou Wang

引用次数: 1

Scale-Up Engineering Super-Energetic Adipose-Derived Mesenchymal Stem Cells with Mitochondrial Transplantation for Tissue Regeneration 大规模工程超能量脂肪间充质干细胞与线粒体移植用于组织再生

MatSciRN: Stem Cell Bioengineering (Topic) Pub Date : 1900-01-01 DOI: 10.2139/ssrn.3855761

Xudong Yao, Yuanzhu Ma, Youguo Liao, Qiulin He, Hongwei Wu, Junxin Lin, Wenyan Zhou, Zongsheng Jiang, Wei Wei, Xiaozhao Wang, M. Björklund, H. Ouyang

{"title":"Scale-Up Engineering Super-Energetic Adipose-Derived Mesenchymal Stem Cells with Mitochondrial Transplantation for Tissue Regeneration","authors":"Xudong Yao, Yuanzhu Ma, Youguo Liao, Qiulin He, Hongwei Wu, Junxin Lin, Wenyan Zhou, Zongsheng Jiang, Wei Wei, Xiaozhao Wang, M. Björklund, H. Ouyang","doi":"10.2139/ssrn.3855761","DOIUrl":"https://doi.org/10.2139/ssrn.3855761","url":null,"abstract":"Stem cell therapies are unsatisfactory due to poor cell survival and engraftment. Stem cell used for therapy must be properly “tuned” for a harsh in vivo environment. Herein, we report that transfer of exogenous mitochondria (mito) to adipose-derived mesenchymal stem cells (ADSCs) can effectively boost their energy levels, enabling efficient cell engraftment. Importantly, the entire process of exogeneous mitochondrial endocytosis is captured by high-content live-cell imaging. Mitochondrial transfer leads to acutely enhanced bioenergetics, with nearly 17% of higher adenosine 5′-triphosphate (ATP) levels in ADSCs treated with high mitochondrial dosage, and further results in altered secretome profiles of ADSCs. Mitochondrial transfer also induced the expression of 334 mRNAs in ADSCs which are mainly involved in signaling pathways related to DNA replication and cell cycle. We propose that increase in ATP and cyclin-dependent kinase (CDK) 1 and 2 expression might be responsible for promoting enhanced proliferation, migration and differentiation of ADSCs in vitro. More importantly, mito-transferred ADSCs display prolonged cell survival, engraftment and horizontal transfer of exogenous mitochondria to surrounding cells in a full-thickness skin defect rat model with improved skin repair compared with non-treated ADSCs. These results demonstrate that intracellular mitochondrial transplantation is a promising strategy to engineer stem cells for tissue regeneration.","PeriodicalId":228505,"journal":{"name":"MatSciRN: Stem Cell Bioengineering (Topic)","volume":"28 10","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114033267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0