Genomics and Applied Biology最新文献

{"title":"miRNA Analysis of Leaves of Zijuan Tea (Camellia sinensis) Based on High throughput Sequencing","authors":"Weihan Song, L. Xia, Yiping Tian, Huibing Jiang, Yunnan Sun, Dehe Liu, Linbo Chen","doi":"10.5376/GAB.2019.10.0001","DOIUrl":"https://doi.org/10.5376/GAB.2019.10.0001","url":null,"abstract":"“Zijuan” is specific tea resource with abundant secondary metabolites, like catechins, anthocyanins, flavonoids, etc. Its biosynthesis is a network of multiple metabolic pathways connected by associated nodes, which is controlled by a variety of structural and regulatory genes. MicroRNAs (miRNAs), as a non-coding RNAs, play important roles in plant growth, development and secondary metabolism by regulating gene expression. In this study, four independent miRNA libraries of bud, second-leaf, open surface leaf and mature-leaf of “Zijuan” tea were constructed and sequenced by high-throughput sequencing. 126 known miRNAs were identified and divided into 26 families, and 119 novel miRNAs were predicted. Based on the transcriptome data of “Zijuan” tea, 724 and 2,285 target genes were predicted for known and novel miRNAs, respectively. The predicted target genes were mostly transcription factors, which included MYB and bHLH transcription factors for regulating the biosynthesis of secondary metabolites -- anthocyanins and flavonoids. All the above results would provide a theoretical basis for further studies on miRNA regulating the development of tea leaf and the biosynthesis of secondary metabolites in Camellia sinensis .","PeriodicalId":223468,"journal":{"name":"Genomics and Applied Biology","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126247790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of COR413 Gene Family in Peach and Its Expression in Low Temperature and LTC Treatment at Postharvest","authors":"Shuaijie Li, Wenna Zhang, Zhigang Zhang, Yaqin Zheng, Z. Liu, Meng Xu","doi":"10.5376/gab.2023.14.0002","DOIUrl":"https://doi.org/10.5376/gab.2023.14.0002","url":null,"abstract":"","PeriodicalId":223468,"journal":{"name":"Genomics and Applied Biology","volume":"122 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132713759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of LDOX Gene Structure Analysis and Construction of Protein Interaction Network in Actinidia arguta","authors":"Zhenpan Liu, Liyuan Lu, Yang Sun, Yue Zhang, Dongsheng Li, Wen-zhong You","doi":"10.5376/gab.2023.14.0001","DOIUrl":"https://doi.org/10.5376/gab.2023.14.0001","url":null,"abstract":"","PeriodicalId":223468,"journal":{"name":"Genomics and Applied Biology","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127079453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparison of Function Annotation Methods over Multiple Meta-omics Data of Microbiota","authors":"Cristopher Reyes Loaiciga, Jing Li","doi":"10.5376/GAB.2020.11.0001","DOIUrl":"https://doi.org/10.5376/GAB.2020.11.0001","url":null,"abstract":"","PeriodicalId":223468,"journal":{"name":"Genomics and Applied Biology","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115939237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and Identification of Cardiomyocyte-specific High Expression Human APE1 Transgenic Mouse","authors":"Jiamei Jiang, Peng Wang, Suhang Li, Zhiqiang Wang, Keng Wu, Runmin Guo","doi":"10.5376/gab.2019.10.0002","DOIUrl":"https://doi.org/10.5376/gab.2019.10.0002","url":null,"abstract":"The aim of this study is to establish transgenic mice that specifically over-express human APE1 incardiac, and to provide a tool animal for studying the relationship between the function and mutation of hAPE1 gene and cardiac development and cardiovascular diseases. A cardiomyocyte-specific hAPE1 transgenic construct containing the α-myosin heavy chain (α-MHC) promoter and human A PE1 (hA PE1) gene was generated. The transgenic vector was constructed by insertion of hAPE1 gene under the α-MHC promoter. The transgenic mice were generated by fertilized egg microinjection followed by embryo transplantation and were all maintained on C57BL/6J genetic background. The genotype of transgenic mice was identified using PCR and the expression levels of hAPE1 in different tissues were detected by Western blotting. The results indicated that cardiomyocyte-specific hAPE1 transgenic construct containing the α-myosin heavy chain (α-MHC) promoter and human APE1 (hAPE1) gene were introduced into fertilized zygotes by microinjection, and then the fertilized zygotes were implanted into the oviduct of female mice, establishing a heart specific high expression hAPE1 transgenic mouse line. 40 offspring were obtained and 15 mice carrying the human APE1 gene was identified by PCR. The heart-specific overexpression of hAPE1 was confirmed by Western blotting assay. The present study successfully obtained cardiomyocyte-specific hAPE1 transgenic mice transgenic mice expressing, which provided a useful tool for studying the function of genes in heart development and cardiovascular disease. and the transgenic mice with cardiac specific overexpression of hAPE1 were obtained. Real-time quantitative PCR and Western blotting were used to detect the expression of mRNA and protein in hAPE1 in the heart of transgenic mice. It was found that the expression level of mRNA and protein in hAPE1 in the heart of transgenic mice increased significantly. Our research showed that we have successfully constructed α - MHC-hAPE1 transgenic mice, which would provide an ideal experimental animal model for studying the detailed mechanism of hAPE1 gene in heart development and cardiovascular disease.","PeriodicalId":223468,"journal":{"name":"Genomics and Applied Biology","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133642955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assembly and Influencing Factors of IMPDH2 Cytoophidia","authors":"Liwei Shi, Weiwei Zhang","doi":"10.5376/GAB.2021.12.0001","DOIUrl":"https://doi.org/10.5376/GAB.2021.12.0001","url":null,"abstract":"Cytoophidium is a \"rod\" or \"ring\" structure composed of metabolically related enzymes. Inosine-5'-monophosphate dehydrogenase ( IMPDH ) is a rate-limiting enzyme that catalyzes the de novo biosynthetic pathway of guanine nucleotides and plays a very important role in cell proliferation, cell differentiation, maintenance of body balance and metabolism. The study found that IMPDH2 gene can be assembled into a special structure of protein polymer fibers, named IMPDH2 cytoophidia, the structure is a natural biological phenomenon related to purine metabolism, as a fine-tuning protein adapted to the intracellular and extracellular environment changes. This article reviews the structure of IMPDH2 and IMPDH2 cytoophidia, the assembly process and the influencing factors of IMPDH2 cytoophidia.","PeriodicalId":223468,"journal":{"name":"Genomics and Applied Biology","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121690482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide Identification and Bioinformatics Analysis of Soybean CHX Gene Family","authors":"Xiaoxi Cai, Yang Shen, Wuhong Zhou, Bowei Jia, Mingzhe Sun, Jinyu Wang, Junkai Yang, Jianwei Li, Xiaoli Sun","doi":"10.5376/gab.2019.10.0003","DOIUrl":"https://doi.org/10.5376/gab.2019.10.0003","url":null,"abstract":"","PeriodicalId":223468,"journal":{"name":"Genomics and Applied Biology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115009242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}