SOJ Pharmacy & Pharmaceutical Sciences最新文献

SOJ Pharmacy & Pharmaceutical Sciences Pub Date : 2021-01-01 DOI: 10.53902/sojps.2021.01.000502

Haba Mohamed Rabei

引用次数: 0

In-Vitro Investigation of CPH Drug-Montmorillonite Clay Interaction CPH与蒙脱土相互作用的体外研究

SOJ Pharmacy & Pharmaceutical Sciences Pub Date : 2021-01-01 DOI: 10.53902/sojps.2021.01.000501

A. M. M Saeed

{"title":"In-Vitro Investigation of CPH Drug-Montmorillonite Clay Interaction","authors":"A. M. M Saeed","doi":"10.53902/sojps.2021.01.000501","DOIUrl":"https://doi.org/10.53902/sojps.2021.01.000501","url":null,"abstract":"The aim of the present work is to explore a naturally occurring clay mineral Montmorillonite (MMT), purified from raw Yemeni bentonite clay, as adsorbent material for ciprofloxacin hydrochloride antibiotic (CPH) in order to investigate the interaction of CPH and MMT. CPH-MMT composites were synthesized and the study design involved the investigation of the effect of three variables, namely; the time, pH and concentration of drug, on the intercalation process. The drug loading and recovery mechanisms were examined and supported by diffusion kinetics laws and adsorption isotherm models. The statistical analysis of the effect of different factors on the adsorption process showed that there was a significant difference in the amount of CPH drug that adsorbed due to variations in these factors. This study showed that the equilibrium time attained after 4 hours and the pH of the drug solution played a crucial role in the intercalation process and the adsorption isotherm was fitted by the Langmuir model with maximum adsorption and followed the pseudo-second-order kinetics. Ciprofloxacin hydrochloride is proved to successfully intercalate into the interlayers of MMT. The intercalation of a drug in MMT follows pseudo-second order kinetics. It is recommended that the overall rate of the adsorption procedure is expected to be controlled by the chemical adsorption process. The adsorption process follows the Langmuir model with a maximum amount of CPH intercalated in MMT being 263.15mg/g. This indicates the homogeneous nature of the MMT surface and the formation of monolayer coverage of CPH on the surface of MMT.","PeriodicalId":22173,"journal":{"name":"SOJ Pharmacy & Pharmaceutical Sciences","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82080988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effect of Amifostine against Etoposide-Induced Genotoxicity Evaluated By the Comet Assays 氨磷汀对依托泊苷遗传毒性的保护作用

SOJ Pharmacy & Pharmaceutical Sciences Pub Date : 2018-06-25 DOI: 10.15226/2374-6866/5/3/00184

M. Shokrzadeh, Nasrin Ghassemi-Barghi

{"title":"Protective Effect of Amifostine against Etoposide-Induced Genotoxicity Evaluated By the Comet Assays","authors":"M. Shokrzadeh, Nasrin Ghassemi-Barghi","doi":"10.15226/2374-6866/5/3/00184","DOIUrl":"https://doi.org/10.15226/2374-6866/5/3/00184","url":null,"abstract":"Etoposide is one of the most effective chemotherapeutic agents used for the treatment of number of neoplasia. However, as a topoisomerase inhibitor, during clinical use several side effects may occur. In addition in several in vivo and in vitro studies etoposide has shown a range of genotoxic effects including mutation induction and inhibition of DNA synthesis. Amifostine, an organic thiophosphate prodrug, has been shown to exert important cyto-protective effects in many tissues. The aim of this study was to explore whether amifostine protects against etoposide-induced genotoxicity in HepG2 cell line. HepG2 cells (25×10 4 cells/well) were cultured in 24-well plates: a control group and three ‘amifostine etoposide groups (pre and cotreatment conditions). Our results show that etoposide induced a noticeable genotoxic effect in HepG2 cells. Amifostine reduced the effects of etoposide significantly in both type of experiment conditions, through reduced the level of DNA damage measured via comet andmicronucleus assay. Furthermore, amifostine decreased the intracellular ROS generation induced by etoposide. It increased also the intracellular GSH levels in HepG2 cells. Altogether, our results suggest a protective action of amifostine against etoposide cytotoxicity and genotoxicity via various pathways. The most protective effect was observed with amifostine when it was administrated 24 h before etoposide treatment.","PeriodicalId":22173,"journal":{"name":"SOJ Pharmacy & Pharmaceutical Sciences","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84940768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Simultaneous Determination of Metformin and Pioglitazone in Presence of Metformin Impurity by Different Spectrophotometric and TLC – Densitometric Methods 分光光度法和薄层色谱-密度法同时测定二甲双胍和吡格列酮中二甲双胍杂质的含量

SOJ Pharmacy & Pharmaceutical Sciences Pub Date : 2018-06-22 DOI: 10.15226/2374-6866/5/3/00183

A. Khorshid, Nessreen S. Abdelhamid, Eglal A. Abdelaleem, Mahmoud Mohsen Amin

引用次数: 5

How to Create Animal Models Using Genetic Knowledge? 如何利用遗传知识创建动物模型?

SOJ Pharmacy & Pharmaceutical Sciences Pub Date : 2018-05-29 DOI: 10.15226/2374-6866/5/3/00182

M. Bourin

引用次数: 0

A Novel Thyroid Hormone Mediated Regulation of HSV-1 Gene Expression and Replication is Specific to Neuronal Cells and Associated with Disruption of Chromatin Condensation. 一种新的甲状腺激素介导的HSV-1基因表达和复制调控是特异性的神经元细胞，并与染色质凝聚的破坏有关。

SOJ Pharmacy & Pharmaceutical Sciences Pub Date : 2014-01-01 DOI: 10.15226/2374-6866/1/1/00106

Feng Chen, Jay Palem, Matthew Balish, Robert Figliozzi, Amakoe Ajavon, S Victor Hsia

{"title":"A Novel Thyroid Hormone Mediated Regulation of HSV-1 Gene Expression and Replication is Specific to Neuronal Cells and Associated with Disruption of Chromatin Condensation.","authors":"Feng Chen, Jay Palem, Matthew Balish, Robert Figliozzi, Amakoe Ajavon, S Victor Hsia","doi":"10.15226/2374-6866/1/1/00106","DOIUrl":"https://doi.org/10.15226/2374-6866/1/1/00106","url":null,"abstract":"<p><p>Previously we showed that thyroid hormone (T3) regulated the Herpes Simplex Virus Type -1 (HSV-1) gene expression and replication through its nuclear receptor TR via histone modification and chromatin remodeling in a neuroblastoma cell line neuro-2a cells (N2a). This observation suggested that T3 regulation may be neuron-specific and have implication in HSV-1 latency and reactivation. In this study, our in vitro latency/reactivation model demonstrated that removal of T3 can de-repress the HSV-1 replication and favor reactivation. Transfection studies and infection assays indicated that HSV-1 thymidine kinase (TK), a key viral gene during reactivation, was repressed by TR/T3 in cells with neuronal origin but not in non-neuronal cells. Additional studies showed that RCC1 (Regulator of Chromosome Condensation 1) was sequestered but efficiently detected upon viral infection in N2a cells. Western blot analyses indicated that addition of T3 repressed the RCC1 expression upon infection. It is likely that diminution of RCC1 upon infection in neuronal cells under the influence of TR/T3 may lead to repression of viral replication/gene expression thus promote latency. Together these results demonstrated that TR/T3 mediated regulation is specific to neuronal cells and differential chromosome condensation may play a critical role in this process.</p>","PeriodicalId":22173,"journal":{"name":"SOJ Pharmacy & Pharmaceutical Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4205947/pdf/nihms-628506.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32772935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6