III. Symposium of Young Researchers on Pharmaceutical Technology, Biotechnology and Regulatory Science最新文献

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Strategies for development of antimicrobial peptides and proteins 抗菌肽和蛋白质的开发策略
Reihaneh Manteghi, K. Kristó, G. Szakonyi, I. Csóka
{"title":"Strategies for development of antimicrobial peptides and proteins","authors":"Reihaneh Manteghi, K. Kristó, G. Szakonyi, I. Csóka","doi":"10.14232/SYRPTBRS.2021.OP8","DOIUrl":"https://doi.org/10.14232/SYRPTBRS.2021.OP8","url":null,"abstract":"Many researches have been done with the aim of overcoming AMPs challenges. Different modification strategies as well as different delivery development ideas were offered by these researches. However, peptide modifications and formulation of peptide delivery systems are challenging tasks and hide several risks. Understanding and evaluating the cause - effect relations within the initial Risk Assessment (RA) step in case of all attributes is novelty since it gives the basis for the experimental design as the next step, and aids the formulation development in order to get the final product in the targeted quality range. It also helps to focus on the resources (human, financial, time) related to the final product quality aimed at. By means of RA method within QbD approach of early pharmaceutical development we monitored the factors with highly risk potential in the PEGylation process and risks such as loosing antimicrobial activity of peptide are prevented. The selection of CQAs, CQAs, QTPPs, CQAs and CPPs/CMAs of a PEGylated PGLa formula was performed and interdependence rating among the QTPP elements and CQAs, as well as among the CPPs and CQAs was performed. This careful theoretical study led to the selection of the right methodologies and materials in the synthesis of PEGylated AMPs and their formulation and consequently resulted in obtaining optimized formulation. In our second work, LYZ encapsulated in a novel polyelectrolyte core-shell nanoparticles through the LBL technique utilized as a carrier system to control the release of protein. The preparation of LYZ NPs was made according to 23 full factorial design with QbD approach. Our aim was to understand the effect of process parameters through the determination of mathematical equations, based on which the optimization parameters can be predicted under different process parameters. The optimization parameters were encapsulation efficiency, particle size, enzyme activity, and the amount of α-helix structure. The nanoparticles were analyzed with transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and circular dichroism (CD) spectroscopy. Based on our results, we found that pH was the most important factor and pH 10 was recommended during the formulation. Enzyme activity and α-helix content correlated with each other very well, and particle size and encapsulation efficiency also showed a very good correlation with each other. The results of the α-helix content of FTIR and CD measurements were very similar for the precipitated lysozyme due to the solid-state of lysozyme.","PeriodicalId":216827,"journal":{"name":"III. Symposium of Young Researchers on Pharmaceutical Technology, Biotechnology and Regulatory Science","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125525138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preformulation studies of ciprofloxacin loaded PVP nanofibers 环丙沙星负载PVP纳米纤维的预配方研究
L. É. Uhljar, S. Kan, N. Radacsi, R. Ambrus
{"title":"Preformulation studies of ciprofloxacin loaded PVP nanofibers","authors":"L. É. Uhljar, S. Kan, N. Radacsi, R. Ambrus","doi":"10.14232/SYRPTBRS.2021.OP25","DOIUrl":"https://doi.org/10.14232/SYRPTBRS.2021.OP25","url":null,"abstract":"Ciprofloxacin is a worldwide-used, broad-spectrum antibiotic with low water-solubility [1]. To earn higher solubility, and better bioavailability, nanofibers were fabricated as an amorphous solid dispersion with the polymer, polyvinylpyrrolidone (PVP). For the production, needle and needleless electrospinning methods were used [2]. The fiber size and morphology were observed by scanning electron microscopy (SEM). Physicochemical properties were characterized by X-ray powder diffraction (XRPD), differential scanning calorimeter (DSC), and Fourier-transform infrared spectroscopy (FTIR). The results proved the amorphous state of the CIP inside the nanofibrous mats. The solubility, in vitro dissolution rate, and in vitro diffusion were remarkably higher in the case of the nanofibers compared with the CIP powder or the physical mixture of the two components. The solubility of the CIP demonstrated a significant increase both in water (pH 6.3) and phosphate buffer solution (pH 7.4). In addition, fastdissolving formulations were developed, while 94±6% of the CIP was released in the first 5 min. Moreover, in vitro diffusion from pH 6.8 to pH 7.4 also showed a notable increase. The stability of the nanofibrous samples was studied by SEM and in vitro dissolution. In conclusion, fast-dissolving formulations were built up which can be further investigated to develop appropriate pharmaceutical forms.","PeriodicalId":216827,"journal":{"name":"III. Symposium of Young Researchers on Pharmaceutical Technology, Biotechnology and Regulatory Science","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114539945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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