Annals of Clinical and Medical Microbiology最新文献

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Respiratory and Gut Microbiota of Children with Cystic Fibrosis: A Pilot Study 囊性纤维化儿童的呼吸和肠道微生物群:一项初步研究

Annals of Clinical and Medical Microbiology Pub Date : 1900-01-01 DOI: 10.47739/acmm-1028

Jannaina Ferreira de Melo Vasco, Carlos A. Riedi, C. Marconi, Keite S. Nogueira, Luiza Souza Rodrigues, Wellington Pine Omori, Rafael R. de Oliveira, Lilian Perreira-Ferrari, Nelson A. Rosário Filho

{"title":"Respiratory and Gut Microbiota of Children with Cystic Fibrosis: A Pilot Study","authors":"Jannaina Ferreira de Melo Vasco, Carlos A. Riedi, C. Marconi, Keite S. Nogueira, Luiza Souza Rodrigues, Wellington Pine Omori, Rafael R. de Oliveira, Lilian Perreira-Ferrari, Nelson A. Rosário Filho","doi":"10.47739/acmm-1028","DOIUrl":"https://doi.org/10.47739/acmm-1028","url":null,"abstract":"Differences in the clinical presentation of cystic fibrosis (CF) may be due to microbiota components and their relationship with the host’s immune system. In this pilot study, we aimed to investigate the composition of the respiratory and gut microbiota of a cohort of clinically stable children with CF, homozygous for the p.Phe508del mutation. Oropharyngeal swabs and stool samples were obtained from these children attending the CF referral clinics at the Hospital of Clinics, Federal University Paraná (CHC – UFPR). Oropharyngeal and gut microbiota were assessed by V3-V4 sequencing of the 16S ribosomal RNA, and bioinformatics analyses were performed using a proprietary pipeline. We identified a total of 456 bacterial taxa belonging to 164 genera, of which 65 (39.6 %) were common to both the respiratory and gastrointestinal tracts. Taxa from eight genera dominated more than 75 % of the microbial composition of both the niches. Among these dominant taxa, only Prevotella spp. were common to both the sites. Overall, the respiratory and gut microbiota were homogeneous among all the patients. Longitudinal studies targeting a larger cohort are important for an improved understanding of how the composition of bacterial communities is related to changes in the clinical status of CF","PeriodicalId":208762,"journal":{"name":"Annals of Clinical and Medical Microbiology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123138039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A Search for Identifying Aerobic Bacteria by Culture and Multiplex PCR in Market Eggs Causing Gastroenteritis and Enteric Fever in Bangladesh 通过培养和多重PCR在孟加拉国市场鸡蛋中鉴定引起肠胃炎和肠热的需氧细菌

Annals of Clinical and Medical Microbiology Pub Date : 1900-01-01 DOI: 10.47739/acmm-1001

J. Fardows, S. Shamsuzzaman

{"title":"A Search for Identifying Aerobic Bacteria by Culture and Multiplex PCR in Market Eggs Causing Gastroenteritis and Enteric Fever in Bangladesh","authors":"J. Fardows, S. Shamsuzzaman","doi":"10.47739/acmm-1001","DOIUrl":"https://doi.org/10.47739/acmm-1001","url":null,"abstract":"To observe the chance of possible transmission of pathogenic bacteria from market egg to the community, potential pathogenic aerobic bacteria were detected from market eggs by culture and multiplex PCR. Egg shells and egg contents of 150 eggs collected from different markets of Dhaka city were tested. Total 145 (96.67%) egg shells yielded growth of bacteria, 23 (15.86%) of them were ESBL producers. Esch. coli was the most common (26.67%) bacteria and 7 (4.67%) were Salmonella spp. Other bacteria were Klebsiella pneumoniae (6.67%), Proteus vulgaris (3.33%), Proteus mirabilis (2%), Providencia rettgeri (15.33%), Providencia alkalifaciens (1.33%), Acinetobacter baumanii (8.67%), Citrobacter freundii (10%), Enterobacter aerogenes (6.67%) Klebsiella oxytoca (4.67%) and Pseudomonas aeruginosa (6.67%). By PCR, 15 (10%) Salmonella spp. was identified from egg shells and the most common serotype was Salmonella Enteritidis (53.33%). No bacteria were detected from egg contents. Most of the bacteria were sensitive to imipenem and colistin. All Salmonella serotypes were sensitive to chloramphenicol, imipenem, gentamicin, ciprofloxacin and ceftriaxone. In conclusion, it can be said that market eggs may be an important source of infection of many gram negative bacteria including Salmonella to the community.","PeriodicalId":208762,"journal":{"name":"Annals of Clinical and Medical Microbiology","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115181740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3