A. Kuria, P. Gathura, L. Kanja, A. Onyuka, Kipyegon Cheruiyot
{"title":"EVALUATION OF THE EFFECTIVENESS OF DIFFERENT ENZYME FORMULATIONS IN THE UNHAIRING PROCESS OF GOATSKINS","authors":"A. Kuria, P. Gathura, L. Kanja, A. Onyuka, Kipyegon Cheruiyot","doi":"10.15677/jallpa.2023.v7i1.23","DOIUrl":"https://doi.org/10.15677/jallpa.2023.v7i1.23","url":null,"abstract":"Due to the high levels of pollution load produced in the leather industry, many studies are being conducted to replace most of the hazardous chemicals used. One of the options is to use enzymes which are biodegradable and considered environmentally friendly. Hides and skins contain many non-collagenous substances that require specific enzymes to remove. The aim of the study was to evaluate the effectiveness of different enzyme formulations in the unhairing process of goatskins. To achieve this, four types of enzymes were purchased from Jian Grace Industries which include amylase (100,000 U/g), protease (200,000 U/g), keratinase (200,000 U/g), and lipase (100,000 U/g). All possible combinations of one, two, three and four enzymes per formulation were prepared by mixing equal amounts per formulation. Parameters such as total solids, dissolved solids and protein content were determined on the affluent by standard procedures. In addition, the percentage weight gain, residual fat content and organoleptic test of the pelt were evaluated. Data were analysed using SPSS statistical packages version 21. ANOVA and t-test was used to test the level of significance (p≤ 0.05). Although several formulations gave complete unhairing, a formulation of keratinase, protease, and lipase (KPL) was selected as the best formulation. This formulation gave a completely unhaired pelt with a residual fat content of 6.4%. The organoleptic tests of all the unhaired pelts had a rating of 7-9. In conclusion, application of more than one enzyme can be a better option in removing non-collagenous components and in the replacement of the use of sodium sulphide in unhairing process.","PeriodicalId":206513,"journal":{"name":"Journal of African Leather and Leather Products Advances","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124511596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wen Huitao, Yu Guofei, Chen Yining, Dan Nianhua, Dan Weihua
{"title":"An antibacterial strategy on chrome-free tanned leather: based on β-cyclodextrin aldehyde derivatives and small molecule","authors":"Wen Huitao, Yu Guofei, Chen Yining, Dan Nianhua, Dan Weihua","doi":"10.15677/jallpa.2021.v6i1.22","DOIUrl":"https://doi.org/10.15677/jallpa.2021.v6i1.22","url":null,"abstract":"Chrome tanning has dominated in leather production up to now, while facing a significant challenge in terms of chromium. Therefore, the chrome-free tanning process becomes an inevitable choice. However, chrome-free tanned leather (CFTL) is poor in antibacterial properties, which limits its practical application in leather production. In this work, a new environment-friendly antibacterial strategy based β-cyclodextrin (β-CD) aldehyde derivatives and ciprofloxacin hydrochloride (CH) as a small molecule model antibacterial agent was developed to address this issue along with improving the comprehensive performances of leather. The structure and properties of obtained leather were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, differential scanning calorimetry, thermogravimetric, and its antibacterial effect against E. coli and S. aureus was investigated. The results showed that compared with CFTL without β-CD modification and CH treatment, the Ts, Td and Tp of the leather with this method possessed increased 5.7°C, 4.9°C, and 6.9°C, respectively. After being treated by CH with 90 days of storage, the antibacterial rates against E. coli and S. aureus still reached 99.8% and 97.4%, respectively, which were 29.9% and 34.4% higher than those without β-CD treatment. It, that antibacterial agent is included in the cavity of β-CD on CFTL, will improve the poor antibacterial durability due to the release of the small molecule antimicrobial agent. These findings, therefore, indicated that a new antibacterial method on CFTL with β-cyclodextrin aldehyde derivatives and ciprofloxacin hydrochloride has potential practical application prospects in leather production.","PeriodicalId":206513,"journal":{"name":"Journal of African Leather and Leather Products Advances","volume":"97 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129823720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}