MicroRNA in ocular pathology最新文献

Predictive monitoring of secondary epidemic waves of COVID-19 in Iran, Russia and other countries 伊朗、俄罗斯等国新冠肺炎二次流行波预测监测

MicroRNA in ocular pathology Pub Date : 2020-08-01 DOI: 10.24075/brsmu.2020.046

DA Kovriguine, SP Nikitenkova

{"title":"Predictive monitoring of secondary epidemic waves of COVID-19 in Iran, Russia and other countries","authors":"DA Kovriguine, SP Nikitenkova","doi":"10.24075/brsmu.2020.046","DOIUrl":"https://doi.org/10.24075/brsmu.2020.046","url":null,"abstract":"In the last decade of April 2020, the second coronavirus epidemic wave in Iran has bloomed. The new wave has started in the vicinity of the critical point, marked by approximately 44,000 infections, where the rate of increase of the primary epidemic that appeared in Iran in mid-February 2020 was the highest. Today, this secondary wave almost has doubled the peak of the primary, and, passing the epidemic threshold of about 70,000 total cases in early June, generated the new third epidemic wave developing unpredictably and dynamically. The purpose of this work was to call into use a simple dynamical system represented by the discrete logistic equation with unknown parameters to predict secondary waves using the official statistical data. The mathematical modelling reveals the secondary epidemic waves in Sweden, the United States, Ukraine, Serbia, Romania, Czech Republic, Portugal, Luxembourg, Poland, and Ecuador. Also, the second waves appear in Russia and other countries. Despite many individual differences in the epidemic spread in different countries, we have traced regularity in the rise of secondary waves. The beginning of each new wave, if focusing on the number of total cases, practically coincides with the time of the maximum growth rate of the previous early epidemic. Thus, the passing through the threshold of the current wave should be the most responsible for strict observance of the rules of self-isolation and other sanitary standards.","PeriodicalId":205913,"journal":{"name":"MicroRNA in ocular pathology","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130566529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypoxia enhances transcytosis in intestinal enterocytes 缺氧促进肠上皮细胞的胞吞作用

MicroRNA in ocular pathology Pub Date : 2020-08-01 DOI: 10.24075/brsmu.2020.049

D. Maltseva, M. Shkurnikov, S. Nersisyan, S. Nikulin, A. Kurnosov, M. Raigorodskaya, A. Osipyants, EA Tonevitsky

引用次数: 0

Complexes of fluconazole with alanine, lysine and threonine: mass spectrometry and theoretical modeling 氟康唑与丙氨酸、赖氨酸和苏氨酸的配合物:质谱分析和理论建模

MicroRNA in ocular pathology Pub Date : 2020-08-01 DOI: 10.24075/brsmu.2020.048

V. Chagovets, N. Starodubtseva, V. Frankevich

{"title":"Complexes of fluconazole with alanine, lysine and threonine: mass spectrometry and theoretical modeling","authors":"V. Chagovets, N. Starodubtseva, V. Frankevich","doi":"10.24075/brsmu.2020.048","DOIUrl":"https://doi.org/10.24075/brsmu.2020.048","url":null,"abstract":"Investigation of the triazole-derived drugs action mechanisms and understanding of their affinity and specificity molecular basis may contribute to the new drugs development. The study was aimed to investigate the triazoles class representative (fluconazole) complexes with amino acids using mass spectrometry, molecular dynamics and ab initio quantum chemistry calculations. During the experimental study, the fluconazole, alanine, lysine and threonine solutions were analyzed by electrospray ionization mass spectrometry and tandem mass spectrometry. The molecular dynamics modeling of the fluconazole–amino acid complexes was performed using the CHARMM force field. The quantum chemistry calculations of the complexes structure and energy parameters were carried out using the density-functional theory by B3LYP calculations (3-21G and 6-311++G** basis sets). Mass spectra indicated that fluconazole formed stable complexes with amino acids in the 1 : 1 stoichiometric ratio. In accordance with the tandem mass spectrometry with varying fluconazole–amino acid associates ion fragmentation energy, the following sequence was obtained: [Fluc + Ala + H]+ < [Fluc + Lys + H]+ < [Fluc + Thr + H]+. The fluconazole–amino acid interaction energy values resulting from the quantum chemistry calculations formed the sequence similar to that obtained by experiment. Thus, as seen in the case of fluconazole–amino acid complexes, it is possible to combine the experimental mass spectrometry studies with quantum chemical modeling for the complexes properties assessment.","PeriodicalId":205913,"journal":{"name":"MicroRNA in ocular pathology","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134130799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rehabilitation of patients with cerebral palsy using hand exoskeleton controlled by brain-computer interface 脑机接口控制手外骨骼在脑瘫患者康复中的应用

MicroRNA in ocular pathology Pub Date : 2020-08-01 DOI: 10.24075/brsmu.2020.047

PD Bobrov, E. Biryukova, B. Polyaev, OA Lajsheva, EL Usachjova, AV Sokolova, DI Mikhailova, KN Dement'eva, I. Fedotova

{"title":"Rehabilitation of patients with cerebral palsy using hand exoskeleton controlled by brain-computer interface","authors":"PD Bobrov, E. Biryukova, B. Polyaev, OA Lajsheva, EL Usachjova, AV Sokolova, DI Mikhailova, KN Dement'eva, I. Fedotova","doi":"10.24075/brsmu.2020.047","DOIUrl":"https://doi.org/10.24075/brsmu.2020.047","url":null,"abstract":"Cerebral palsy (CP) is one of the most severe central nervous system diseases in childhood associated with motor impairment. The study was aimed to assess the efficiency of the complex comprising brain-computer interface (BCI) and hand exoskeleton as an instrument for the motor function recovery in patients with CP complementing the essential therapy. The Fugl-Meyer Assessment scale, ARAT test and Jebsen–Taylor function test were used in 14 children and adolescents for the motor function improvement assessment after the therapy complemented by 7–10 BCI-exoskeleton based procedures. The EEG mu-rhythm sources properties during the motor imagery BCI control were studied. After the procedures completion, the significant improvement of the Fugl-Meyer Assessment scale score (7 (2; 11) for hand active movements; 4.5 (1; 6) for proximal arm and 2.5 (0; 5) for hand), ARAT test score (7.5 (1; 31) for total score, 1.5 (0; 12) for grasp movement and 1.5 (0; 8) for grip movement), as well as significantly different from the zero execution time reduction in three out of seven Jabsen–Taylor function test items (–1 (–4.13; 0.25) for simulated feeding; –1 (–2; 0) for moving light and heavy cans) were identified. The average BCI detection level was 0.51 (0.45; 0.54) (max = 0.70). In most EEG recordings the mu-rhythm sources were detected, both for intact and affected hemispheres. The mu-rhythm desynchronization associated with motor imagery was observed, significantly affecting the BCI accuracy. The results obtained indicate that the use of BCI-exoskeleton complex effectively complements the standard rehabilitation methods for children with CP, and suggest that its clinical effectiveness in individuals with CP may be proven by enrollment of more patients.","PeriodicalId":205913,"journal":{"name":"MicroRNA in ocular pathology","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124856502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Information model of post stroke rehabilitation conception 脑卒中后康复概念的信息模型

MicroRNA in ocular pathology Pub Date : 2020-08-01 DOI: 10.24075/brsmu.2020.051

PA Rybakova, YuI Koroleva, G. Ivanova, T. Zarubina

引用次数: 0

Thrombogenicity in patients with ischemic stroke and pre-existing polycythemia vera 缺血性卒中和真性红细胞增多症患者的血栓形成性

MicroRNA in ocular pathology Pub Date : 2020-08-01 DOI: 10.24075/brsmu.2020.052

M. Tanashyan, A. Shabalina, E. Roitman, T. Vavilova, P. Kuznetsova

{"title":"Thrombogenicity in patients with ischemic stroke and pre-existing polycythemia vera","authors":"M. Tanashyan, A. Shabalina, E. Roitman, T. Vavilova, P. Kuznetsova","doi":"10.24075/brsmu.2020.052","DOIUrl":"https://doi.org/10.24075/brsmu.2020.052","url":null,"abstract":"Thrombogenicity and its causes in patients with ischemic stroke (IS) and pre-existing polycythemia vera (PV) is a significant clinical concern. The aim of this study was to identify the range of factors associated with increased thrombogenicity in patients with IS and pre-existing PV. We performed a physical examination and laboratory tests on 127 patients in the hyperacute stroke stage and 16-18 months after. Of them, 68 patients had PV (the main group) and 59 did not have this condition (the comparison group). Laboratory tests were conducted to evaluate blood rheology, hemostasis, endothelial function, angiogenesis, proinflammatory cytokine levels; we also tested patients for the presence of the V617F mutation in the JAK2 gene and analyzed the contribution of all studied parameters to the development of thrombotic and hemorrhagic complications. We found that the neurological picture did not differ between the groups: mean NIHSS scores were 12 and 13 points, respectively. Morphological and functional characteristics of red blood cells and platelets, hemostasis and cytokine profiles were compared between patients with IS and pre-existing PV and the comparison group. One of the key elements in potentiating thrombotic complications in patients with IS and PV was JAK2 V617F allele burden. The obtained data suggest the cumulative effect of the identified factors promoting thrombus formation in post-stroke patients with PV and overpowering the effect of antiplatelet therapy.","PeriodicalId":205913,"journal":{"name":"MicroRNA in ocular pathology","volume":"239 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122933305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

New in vitro model to evaluate kinetics of antimycobacterial drug release from bioresorbable polymeric carriers 新的体外模型评估抗细菌药物从生物可吸收聚合物载体释放动力学

MicroRNA in ocular pathology Pub Date : 2020-08-01 DOI: 10.24075/brsmu.2020.050

S. Andreevskaya, T. Smirnova, E. Antonov, L. Chernousova, S. Bogorodsky, E. Larionova, V. Popov, A. Ergeshov

{"title":"New in vitro model to evaluate kinetics of antimycobacterial drug release from bioresorbable polymeric carriers","authors":"S. Andreevskaya, T. Smirnova, E. Antonov, L. Chernousova, S. Bogorodsky, E. Larionova, V. Popov, A. Ergeshov","doi":"10.24075/brsmu.2020.050","DOIUrl":"https://doi.org/10.24075/brsmu.2020.050","url":null,"abstract":"Sustained-release drugs against tuberculosis are a promising approach to therapy since they positively affect patient compliance with long regimens, especially when it comes to the multidrug-resistant form of the disease. Conventional UV-visible spectroscopy does not work well with multicomponential culture media used for growing M. tuberculosis. The aim of this study was to develop a method for evaluating the kinetics of anti-tuberculosis drug released from bioresorbable polymeric carriers suitable for screening a wide range of encapsulated prolonged-release drugs and identifying the best performing candidate. While studying the growth dynamics of the laboratory susceptible strain M. tuberculosis H37Rv in the presence of different levofloxacin concentrations (from 0.03 to 0.4 μg/ml), we developed a model, which is essentially a set of 2 parallel experiments evaluating the kinetics of drug release into the culture medium. The results of these 2 experiments conducted on 3 encapsulated forms of levofloxacin loaded onto bioresorbable polymeric PLGA carriers (particles sized 50 μm and 100 μm and the matrix) revealed that release kinetics of the drug largely depended on the type of polymeric carrier. The best encapsulation of the antibiotic and its gradual release into the culture medium was observed for the matrix. All experiments were run in 3 replicates. The obtained data were analyzed using descriptive statistics.","PeriodicalId":205913,"journal":{"name":"MicroRNA in ocular pathology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124484556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in EEG patterns in the α-frequency band following BCI-based therapy in children with cerebral palsy 脑机接口治疗后脑瘫患儿α-频带脑电图的变化

MicroRNA in ocular pathology Pub Date : 2020-07-16 DOI: 10.24075/brsmu.2020.043

N. Larina, M. Nacharova, L. Korsunskaya, S. Vlasenko, V. Pavlenko

{"title":"Changes in EEG patterns in the α-frequency band following BCI-based therapy in children with cerebral palsy","authors":"N. Larina, M. Nacharova, L. Korsunskaya, S. Vlasenko, V. Pavlenko","doi":"10.24075/brsmu.2020.043","DOIUrl":"https://doi.org/10.24075/brsmu.2020.043","url":null,"abstract":"It was demonstrated previously that neurorehabilitation with the BCI-controlled robotic device Exohand-2 combined with conventional therapeutic modalities resulted in significant motor improvement in children with cerebral palsy. However, EEG records were not analyzed in the previous study. The aim of this paper was to describe the reactivity patterns of the EEG α-rhythm during a series of 10 BCI-based neurorehabilitation sessions. The study was carried out in 32 boys and girls aged 10 to 18 years with right- or left-side hemiparesis. EEG was recorded from 21 electrodes at rest and during kinesthetic imagery of finger extension. During the first session, patterns of α-rhythm reactivity during motor imagery differed between patients with left- and right-side hemiparesis. The differences were statistically significant at Р2 during left hand movement rehearsal (F1, 30 = 5.10; p < 0.05). During the final session, the pattern of α-rhythm reactivity was different: synchronization was taken over by desynchronization at some electrode sites, suggesting increased activity of the neocortex. The most conspicuous EEG changes were observed in children with left-side hemiparesis (F20, 300 = 1.84; p < 0.05). By the end of the rehabilitation course, the differences between patients with left-and right-side hemiparesis became much less pronounced. Rearrangements in the EEG patterns in the α-frequency band can be regarded as signs of beneficial reorganization of neural circuits responsible for planning and executing complex hand movements.","PeriodicalId":205913,"journal":{"name":"MicroRNA in ocular pathology","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132661899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

MicroRNA and vascular pathology of the eye 眼部的MicroRNA与血管病理

MicroRNA in ocular pathology Pub Date : 2020-07-15 DOI: 10.24075/brsmu.2020.042

L. Moshetova, S. Usharova, KI Turkina, D. Sychev, IN Saburina

引用次数: 0

Gut microbiota assessment in Moscow long-livers using next generation sequencing 使用下一代测序评估莫斯科长寿者的肠道微生物群

MicroRNA in ocular pathology Pub Date : 1900-01-01 DOI: 10.24075/brsmu.2020.044

D. Kashtanova, NS Klimenko, I. Strazhesko, ON Tkacheva, E. Starikova, O. Glushchenko, D. Gudkov, E. Ilina

{"title":"Gut microbiota assessment in Moscow long-livers using next generation sequencing","authors":"D. Kashtanova, NS Klimenko, I. Strazhesko, ON Tkacheva, E. Starikova, O. Glushchenko, D. Gudkov, E. Ilina","doi":"10.24075/brsmu.2020.044","DOIUrl":"https://doi.org/10.24075/brsmu.2020.044","url":null,"abstract":"Demographic aging poses a challenge to the medical community, pressing for research into the biological factors promoting longevity and its features. Below, we look at the gut microbiota as one of such factors. The aim of this non-longitudinal study was to profile the gut microbiota of centenarians and to compare it with that of relatively healthy, younger Moscow residents. The study recruited 20 people aged 97–100 years (mean age 98 ± 1 year); the control group consisted of 92 individuals aged 53 ± 13 years. For each stool sample, the variable V3–V4 regions of the microbial 16S rRNA gene were sequenced. Primary analysis, read filtering and taxonomic identification were conducted in the QIIME 1.9 environment; reconstruction of metabolic pathways was aided by PICRUSt. Statistical analysis was performed by means of Python v. 3.2. A few differences were detected between the gut microbiota of centenarians and younger individuals: Bifidobacterium (p = 0.026) and Coprococcus eutactus (р = 0.026) were more abundant in centenarians, whereas Bacteroides (p = 0.003) and Prevotella (р = 0.002) were better represented in younger participants. The potential for butyric acid synthesis was higher in the group of centenarians (p = 0.048). Surprisingly, the gut microbiota of centenarians was more diverse and surprisingly beneficial for advanced age. Besides, the gut microbiota of centenarians might have more pronounced anti-inflammatory potential due to its ability to better synthesize butyric acid.","PeriodicalId":205913,"journal":{"name":"MicroRNA in ocular pathology","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127115536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0