Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis最新文献

Front Matter: Volume 10873 封面:卷10873

Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis Pub Date : 2019-05-24 DOI: 10.1117/12.2532072

引用次数: 0

Cytopathology diagnosis by multiplexed plasmonic biomarkers (Conference Presentation) 多路等离子体生物标志物的细胞病理学诊断(会议报告)

Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis Pub Date : 2019-03-07 DOI: 10.1117/12.2510274

S. Patskovsky, Mengjiao Qi, C. Darviot, Lu Wang, Audrey Nsamela, Daniel Tomasso, M. Meunier

引用次数: 0

DO-SRS imaging of metabolic dynamics in animals (Conference Presentation) 动物代谢动力学的DO-SRS成像(会议报告)

Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis Pub Date : 2019-03-04 DOI: 10.1117/12.2507058

Lingyan Shi, C. Zheng, Yihui Shen, Wei Min

{"title":"DO-SRS imaging of metabolic dynamics in animals (Conference Presentation)","authors":"Lingyan Shi, C. Zheng, Yihui Shen, Wei Min","doi":"10.1117/12.2507058","DOIUrl":"https://doi.org/10.1117/12.2507058","url":null,"abstract":"Understanding the dynamics of metabolism in a multicellular organism is essential to unraveling the mechanistic basis of many biological processes. It is the synthesis, transformation and degradation of biomolecules (the definition of metabolism) that carry out the genetic blueprint, which cannot be imaged in vivo by using traditional methods. In the present work, we developed a new method that combines D2O probing and Stimulated Raman Scattering microscopy (DO-SRS) to visualize metabolic dynamics in live animals. The enzymatic incorporation of D2O-derived deuterium (D) into biomolecules will generate carbon-deuterium (C-D) bonds in macromolecules. Within the broad vibrational spectra of C-D bonds, we discover lipid-, protein-, and DNA-specific Raman shifts and develop spectral unmixing methods to obtain C-D signals with macromolecular selectivity. We obtained new biological insights in several studies such as the spatial dependence of lipogenic activities of sebaceous glands, specific myelination timing of the developing mouse brain, differential protein and lipid metabolism in germline development of C. elegans as well as its aging process, the spatial constrain for the distribution of newly synthesized yolk proteins in aged C. elegans, the prevalence of protein biosynthesis and the lack of lipogenesis in zebrafish embryos, and intratumoral metabolic heterogeneity. In summary, we demonstrated that our current DO-SRS method is better than other deuterium-labeled carbon substrate in monitoring and imaging metabolic activities. This technique can track specifically de novo lipogenesis, image in vivo protein biosynthesis without tissue bias, and can simultaneously image spatial temporal dynamics of lipid and protein.","PeriodicalId":194764,"journal":{"name":"Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis","volume":"139 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127462001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical biopsy of vocal folds during phonation using parallel OCT (Conference Presentation) 平行OCT发声过程中声带光学活检(会议报告)

Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis Pub Date : 2019-03-04 DOI: 10.1117/12.2510479

G. Maguluri, D. Mehta, J. Kobler, Jesung Park, N. Iftimia

引用次数: 0

Perspectives of optical diagnosis with vector light beams (Conference Presentation) 矢量光束光学诊断的展望(会议报告)

Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis Pub Date : 2019-03-04 DOI: 10.1117/12.2509145

A. Doronin, N. Vera, J. Staforelli, T. Novikova, I. Meglinski

{"title":"Perspectives of optical diagnosis with vector light beams (Conference Presentation)","authors":"A. Doronin, N. Vera, J. Staforelli, T. Novikova, I. Meglinski","doi":"10.1117/12.2509145","DOIUrl":"https://doi.org/10.1117/12.2509145","url":null,"abstract":"In turbid tissue-like scattering medium the conventional polarized light, scattered multiple number of times, is depolarized, and the depolarization rate depends strongly on the size and shape of scattering particles, as well as on the number of scattering events. In fact, the structure of light can be more complicated when the polarization of light across the laser beam can be radially or azimuthally polarized and carry orbital angular momentum. When these vector light beams, known as cylindrical vector beam (CVB) and Laguerre-Gaussian (LG) beams, propagates in turbid tissue-like scattering medium, either anisotropic or inhomogeneous, the spin or angular momentum are changed that leads to spin-orbit interaction. Such a spin-orbit interaction leads to the mutual influence of the polarization and the trajectory of the light propagation. We investigate the applicability of using CVB and LG beams for optical biopsy. In current presentation propagation of CVB and LG beams in anisotropic turbid tissue-like scattering media is considered in comparison to conventional Gaussian beams. We demonstrate that by applying CVB and LG beams the sensitivity of the conventional polarimetry-based approach is increased at least twice in comparison with the experiments utilizing ‘simple’ Gaussian polarized light. The results of the study suggest that there is a high potential in application of vector light beams in tissue diagnosis.","PeriodicalId":194764,"journal":{"name":"Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126721530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

OCT for core biopsy guidance: a rabbit study (Conference Presentation) OCT用于核心活检指导:一项兔研究(会议报告)

Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis Pub Date : 2019-03-04 DOI: 10.1117/12.2508135

N. Iftimia, G. Maguluri, Jesung Park, S. Sabir

引用次数: 0

Machine learning assisted blood vessel segmentation in laser speckle imaging (Conference Presentation) 激光散斑成像中机器学习辅助血管分割(会议报告)

Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis Pub Date : 2019-03-04 DOI: 10.1117/12.2510378

A. Benmergui, J. Drotleff, T. Pan, Josh Zwiebel, I. Kuznetsov, I. Meglinski, A. Harmelin, V. Kalchenko

引用次数: 0

Cholesterol esterification inhibition suppresses prostate cancer metastasis by impairing the Wnt/β-catenin pathway (Conference Presentation) 胆固醇酯化抑制通过破坏Wnt/β-catenin通路抑制前列腺癌转移(会议报告)

Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis Pub Date : 2019-03-04 DOI: 10.1117/12.2506635

Hyeon Jeong Lee, Ji‐Xin Cheng

引用次数: 1