Fluorescent Materials for Cell Imaging最新文献

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Semiconductor Quantum Dots for Cell Imaging 半导体量子点用于细胞成像

Fluorescent Materials for Cell Imaging Pub Date : 1900-01-01 DOI: 10.1557/PROC-1237-TT06-01

Zoraida P. Aguilar, Hengyi Xu, B. Jones, John D. Dixon, Andrew Z. Wang

{"title":"Semiconductor Quantum Dots for Cell Imaging","authors":"Zoraida P. Aguilar, Hengyi Xu, B. Jones, John D. Dixon, Andrew Z. Wang","doi":"10.1557/PROC-1237-TT06-01","DOIUrl":"https://doi.org/10.1557/PROC-1237-TT06-01","url":null,"abstract":"Nanotechnology is currently undergoing unprecedented development in various fields. There has been a widespread interest in the application of nanomaterials in medicine with its promise of improving imaging, diagnostics, and therapy. The recent advances in engineering and technology have led to the development of new nanoscale platforms such as quantum dots, gold nanocrystals, superparamagnetic nanocrystals, and other semiconductor nanoparticles. Literature on the applications of quantum dots in life sciences has recently increased in number. This may have led to predictions that nanotechnology in life sciences research will contribute $3.4 billion by 2010 while institutions have predicted that the market for nanotechnology and corresponding products will reach $1 trillion in 2012 (1). Ocean NanoTech is at the height of developmental stages of nanoparticle production for biological applications. Ocean’s high quantum-yield quantum dots (QDs) is currently being tested and used for cell imaging, as wells as for the detection of proteins, DNA, whole cells, and whole organisms. Imaging of cells involves conjugation of QDs to highly sensitive and specific antibody to form QD˜Ab conjugates that attach to specific protein target on the cell surface. Attachment of the QD˜Ab on the cell surface allows imaging of the cell under a fluorescence microscope. QD based imaging can be used in a multiplex immunoassay detection of several types of cells (or microorganisms) in a single sample when several size tunable quantum dots are used as reporter probes. We report the QD imaging of breast cancer cells. Using the breast cancer cell line SK-BR3, which expresses high levels of her2 antigens on the cell surface, anti-her2 were conjugated to Ocean’s quantum dots, QSH620. To eliminate non-specific binding of the QD˜20Ab Ocean’s super blocking buffer BBB and BBG were used. Preliminary results of in vitro studies indicated that QD based systems can be used to image cells. We anticipate that this system can be transferred to in vivo detection.","PeriodicalId":193817,"journal":{"name":"Fluorescent Materials for Cell Imaging","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128665062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescent Materials for Cell Imaging 细胞成像荧光材料

Fluorescent Materials for Cell Imaging Pub Date : 1900-01-01 DOI: 10.1007/978-981-15-5062-1

Fu‐Gen Wu

引用次数: 1

Lanthanide-Based Upconversion Nanoparticles for Bioimaging Applications 基于镧系元素的上转换纳米粒子在生物成像中的应用

Fluorescent Materials for Cell Imaging Pub Date : 1900-01-01 DOI: 10.1007/978-981-15-5062-1_6

Youbin Li, S. Zeng, J. Hao

引用次数: 0