Czech Chemical Society Symposium Series最新文献

LC-MS Lipidomic Analysis of Macrophages 巨噬细胞的 LC-MS 脂质体分析

Czech Chemical Society Symposium Series Pub Date : 2024-05-13 DOI: 10.54779/ccsss20240066

Kateřina Pražáková, Vladimír Vrkoslav, Štěpán Strnad, B. Muffová, S. Kauerova, Jiří Froněk, I. Králová Lesná, Rudolf Poledne, David Sýkora

{"title":"LC-MS Lipidomic Analysis of Macrophages","authors":"Kateřina Pražáková, Vladimír Vrkoslav, Štěpán Strnad, B. Muffová, S. Kauerova, Jiří Froněk, I. Králová Lesná, Rudolf Poledne, David Sýkora","doi":"10.54779/ccsss20240066","DOIUrl":"https://doi.org/10.54779/ccsss20240066","url":null,"abstract":"Currently, an increasing incidence of cardiovascular diseases is observed in the population. Factors contributing to their development are obesity and pro-inflammatory processes occurring in the adipose tissue. Macrophages, cells of the non-specific immune system, which can be divided into pro-inflammatory and anti-inflammatory, play a significant role in these processes. For a better understanding of these phenomena, it is necessary to analyze the lipidome of macrophages of the two mentioned groups. The presented work was focused on the optimization of selected parameters of the LC-MS method for the analysis of 71 lipids from 16 lipid classes. Best results were achieved with column Acquity UPLC BEH C18 (1.7 µm, 2.1 mm × 100 mm) operating at the flow rate of 300 μL min–1 and temperature of 55 °C. The optimal desolvation temperature was 350 °C and the gradient elution started at 0 % of solvent B in the mobile phase. Retention dependencies were characterized for individual lipid classes. They were successfully fitted with a linear model (R2 > 0.99) and subsequently used to confirm the accuracy of lipid identification. The optimized LC-MS method was also used to analyze the real samples of macrophages isolated from the adipose tissue of kidney donors. Samples were provided by Institute for Clinical and Experimental Medicine. The principal component analysis gave insight into the division of macrophages according to their type. The volcano plot showed a significant increase in the concentration of five lipids in the case of pro-inflammatory macrophages.","PeriodicalId":193810,"journal":{"name":"Czech Chemical Society Symposium Series","volume":"54 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140983568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solid Bismuth Drop Electrode – A New Tool for Voltammetric Determination of Electrochemically Reducible Organic Compounds 固体滴铋电极--电化学还原性有机化合物伏安法测定的新工具

Czech Chemical Society Symposium Series Pub Date : 2024-05-13 DOI: 10.54779/ccsss20240061

Johanka Jarošová, V. Vyskočil

{"title":"Solid Bismuth Drop Electrode – A New Tool for Voltammetric Determination of Electrochemically Reducible Organic Compounds","authors":"Johanka Jarošová, V. Vyskočil","doi":"10.54779/ccsss20240061","DOIUrl":"https://doi.org/10.54779/ccsss20240061","url":null,"abstract":"Solid bismuth drop electrode (SBiDE) is a new working electrode commercially available on the Czech market from 2020 by the company Metrohm. The aim of this work was to verify the applicability of the SBiDE in the voltammetric determination of a model organic substance. According to the available information, this is the very first published work on this topic. Metronidazole (an antibiotic used to treat diseases caused by both Gram-positive and Gram-negative anaerobic bacteria) was chosen in this study as a model drug representing electrochemically reducible biologically active compounds. Under optimum conditions (Britton-Robinson buffer of pH 12.0 used as the supporting electrolyte and no electrochemical regeneration of the working electrode surface), a linear calibration dependence of metronidazole was recorded in the concentration range from 1 to 600 μmol L–1, with the limits of detection (LOD) and quantification (LOQ) of 0.41 μmol L–1 and 1.4 μmol L–1, respectively. The newly developed differential pulse voltammetric (DPV) method has also been successfully applied in the determination of metronidazole in various dosage forms.","PeriodicalId":193810,"journal":{"name":"Czech Chemical Society Symposium Series","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140983972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of the Applicability of Colloidal Solutions of Gold and Silver Nanoparticles for Surface-enhanced Raman Spectroscopy of Blood Plasma 金、银纳米粒子胶体溶液在血浆表面增强拉曼光谱中的适用性研究

Czech Chemical Society Symposium Series Pub Date : 2022-11-02 DOI: 10.54779/ccsss20220327

Jakub Harvalík, Kateřina Hrubešová, Ondřej Vrtělka, V. Setnička

{"title":"Study of the Applicability of Colloidal Solutions of Gold and Silver Nanoparticles for Surface-enhanced Raman Spectroscopy of Blood Plasma","authors":"Jakub Harvalík, Kateřina Hrubešová, Ondřej Vrtělka, V. Setnička","doi":"10.54779/ccsss20220327","DOIUrl":"https://doi.org/10.54779/ccsss20220327","url":null,"abstract":"This work focuses on the preparation of colloidal solutions of Au and Ag nanoparticles and verification of their applicability for surface-enhanced Raman scattering spectroscopy of blood plasma. The composition of blood plasma may change due to pathobiochemical processes associated with disease development. Analysis of blood plasma might reveal these changes and thus help in the diagnostics process of various diseases. Raman spectroscopy appears to be an ideal method for rapid and non-destructive analysis of blood plasma. Moreover, surface-enhanced Raman scattering spectroscopy might allow the detection of very low levels of potential disease-specific markers. In this work, the prepared colloidal solutions of Au and Ag nanoparticles were first characterized by UV‑VIS absorption spectroscopy and their enhancing capabilities were verified using riboflavin as a model analyte. Subsequently, optimization of the measurement conditions for surface-enhanced Raman spectroscopy of blood plasma was performed. The results show that at the excitation wavelength of 785 nm, significantly higher enhancement is achieved when using Ag nanoparticles. In the case of Au nanoparticles, the enhanced blood plasma spectrum was successfully detected only once, and the addition of an aggregating agent (NaCl) was required.","PeriodicalId":193810,"journal":{"name":"Czech Chemical Society Symposium Series","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127641621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Protein Biomarkers Using Lateral Flow Tests with Photon-upconversion Nanoparticles 利用光子上转换纳米颗粒横向流动试验检测蛋白质生物标志物

Czech Chemical Society Symposium Series Pub Date : 2022-11-02 DOI: 10.54779/ccsss20220335

Eliška Macháčová, Z. Farka

{"title":"Detection of Protein Biomarkers Using Lateral Flow Tests with Photon-upconversion Nanoparticles","authors":"Eliška Macháčová, Z. Farka","doi":"10.54779/ccsss20220335","DOIUrl":"https://doi.org/10.54779/ccsss20220335","url":null,"abstract":"Rapid and sensitive detection of clinically important analytes is crucial for early disease diagnosis and initiation of treatment. Immunochemical assays are widely used to determine the presence and concentration of analytes in various complex media due to their high specificity provided by antibodies. Recently, lateral flow immunoassays (LFIAs) are becoming one of the most popular forms of immunochemical assays. LFIAs are test strips based on nitrocellulose membranes, and their most significant advantage is that there is no need for laboratory equipment to perform the assay enabling their use for point-of-care testing. Most LFIAs are based on gold nanoparticles; however, they often limit the resulting sensitivity of LFIAs. Our work focused on photon-upconversion nanoparticles (UCNPs) as a sensitive alternative label. UCNPs are lanthanide-based nanocrystals exhibiting anti-Stokes luminescence (excitation by the NIR laser and detection in the Vis region), which allows detection without optical background interference. We have developed an LFIA assay for human serum albumin (HSA) with UCNPs as a label. Using a specific anti-HSA antibody and a control anti-mouse antibody, various LFIA parameters were optimized. The immobilization of antibodies was done using acetate buffer with 1% methanol, glass membrane ST17 was used as a conjugate pad, and Tris buffer with 5% sucrose was used to stabilize the particles within the conjugate pad. The choice of a different nitrocellulose membrane had a relatively small effect on assay performance; the best results were achieved with membrane 120 HP Plus Thick. Our work has successfully demonstrated the potential of using UCNPs as sensitive labels in LFIA tests.","PeriodicalId":193810,"journal":{"name":"Czech Chemical Society Symposium Series","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134185498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Transesterification Derivatization Reaction for GC-MS Analysis 酯交换衍生化反应在GC-MS分析中的应用

Czech Chemical Society Symposium Series Pub Date : 2022-11-02 DOI: 10.54779/ccsss20220319

Adam Březina, Adéla Dořmanová, P. Kukučka, P. Lubal, M. Farková

引用次数: 0

The Choice of Solvent for the Extraction of ᴅ-panthenol from Samples of Body Milks 人体乳样品中甲醚泛醇提取溶剂的选择

Czech Chemical Society Symposium Series Pub Date : 2022-11-02 DOI: 10.54779/ccsss20220340

B. Simkova, K. Hroboňová, Andrea Špačková

引用次数: 0

The Application of Ultrasonic Assisted Extraction in Analysis Cinnamon and Food Products 超声波辅助提取在肉桂及食品分析中的应用

Czech Chemical Society Symposium Series Pub Date : 2022-11-02 DOI: 10.54779/ccsss20220323

Kristína Čišovská, K. Hroboňová

引用次数: 0

Influence of Base Material and Addition of Complexing Agent to Deposition Bath for Galvanic Preparation of SERS-active Layers and Spectra of Riboflavin 基底材料和络合剂对电法制备sers活性层的影响及核黄素的光谱

Czech Chemical Society Symposium Series Pub Date : 2022-11-02 DOI: 10.54779/ccsss20220331

Adéla Koryťáková, Oleksandr Volochanskyi, M. Král, Marie Švecová

{"title":"Influence of Base Material and Addition of Complexing Agent to Deposition Bath for Galvanic Preparation of SERS-active Layers and Spectra of Riboflavin","authors":"Adéla Koryťáková, Oleksandr Volochanskyi, M. Král, Marie Švecová","doi":"10.54779/ccsss20220331","DOIUrl":"https://doi.org/10.54779/ccsss20220331","url":null,"abstract":"SERS spectroscopy remains not a well-established analytical technique mainly because of the complicated preparation of SERS-active substrates, which often have low reproducibility. For this reason, the development of new or optimization of existing substrates is important. For testing the SERS activity of these substrates, the so-called model analytes are commonly used. One of them is riboflavin, which is utilized for its non-toxicity and suitable optical properties. \u0000This work is focused on optimizing the enhancing properties of galvanically prepared silver substrates. The influence of the base material (Al and its alloys, Zn) and of the addition of a complexing agent (EDTA) into the Ag deposition bath on the spectral response of riboflavin was evaluated. Each prepared substrate was characterized by scanning electron microscopy, and the SERS spectra were collected using an excitation wavelength of 1064 nm. All data were subsequently processed and interpreted using multivariate statistics, namely principal component analysis. The most universal and suitable base material for the preparation of Ag substrate for riboflavin detection was the AlMg alloy. The addition of a complexing agent to the deposition bath changed the morphology of the prepared substrates and the highest spectral response of riboflavin was obtained by adding 0.55 mM EDTA. The intensity of riboflavin spectral response was comparable to the value obtained using substrates prepared in the Ag bath, but the substrates prepared in the AgNO3 solution achieved a weaker enhancement of the spectral response.","PeriodicalId":193810,"journal":{"name":"Czech Chemical Society Symposium Series","volume":"212 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133462186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0