Recent Research Advances in Biology Vol. 10最新文献

{"title":"Nannochloropsis gaditana as a Source of Novel Antitumor Compounds","authors":"Rafael Carrasco-Reinado, Almudena Escobar-Niño, F. J. Fernández‐Acero","doi":"10.9734/BPI/RRAB/V10/10138D","DOIUrl":"https://doi.org/10.9734/BPI/RRAB/V10/10138D","url":null,"abstract":"During the last decades, proteomics approaches has become in a fundamental tool to unravel the proteins dynamic of biological processes, being an essential technique for basic and applied research [1-4]. Diverse bioinformatic tools are required to manage and explore the huge amount of information obtained from a single proteomics experiment.  To gain insight into potential applications of the identified proteins, a novel approach named \"Applied Proteomics\" has been developed by comparing the obtained protein information with the existing patents database. \u0000The development of massive sequencing technology and MS/MS improvements has allowed the application of proteomic of non-model microorganisms, which have been deeply described as a novel source of metabolites [5-8]. Between them, Nannochloropsis gaditana that has been pointed out as an alternative source of biomolecules [9-13]. Recently, our research group has reported the first complete proteome analysis of this microalga [14], which was analysed using the applied proteomics concept with the identification of 488 proteins with potential industrial applications. To validate our approach, we select UCA01 protein from prohibitin family. The recombinant protein showed its antitumor activity against two tumor cell lines, Caco2 (colon adenocarcinoma) and HepG-2 (hepatocellular carcinoma), proving that proteome data has been transformed into relevant biotechnological information. \u0000In summary, the framework of the European Union “Blue growth” politicies, the revalorization of microalgae biomass is crucial point. From Nannochloropsis gaditana has been developed a new tool against cancer, the protein named UCA01. This protein has selective effect inhibiting the growth of tumour cells, while does not show any effect over control cells. This approach describes the first practical approach to transform proteome information in a potential industrial application, named “applied proteomics”. It is based in a novel bio-algorism, which is able to identified proteins with potential industrial applications. From hundreds of proteins described in the proteome of N. gaditana, the bio-algorism identified over 400 proteins with potential use; one of them was selected as UCA01, “in vitro” and its potential prove it against cancer. The potential applications from this approach are unpredictable.","PeriodicalId":185276,"journal":{"name":"Recent Research Advances in Biology Vol. 10","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130452195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Developing a Novel Parameter Estimation Method for an Agent-Based Model in Immune System Simulation Using History Matching: A Case Study on Influenza a Virus Infection","authors":"Le Zhang, Tingting Li","doi":"10.9734/BPI/RRAB/V10/9995D","DOIUrl":"https://doi.org/10.9734/BPI/RRAB/V10/9995D","url":null,"abstract":"","PeriodicalId":185276,"journal":{"name":"Recent Research Advances in Biology Vol. 10","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121183750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental Biology of Cardiochiles nigricollis Cameron a Larval Endo-parasitoid of Rice Leaffolders","authors":"K. Behera","doi":"10.9734/BPI/RRAB/V10/2633F","DOIUrl":"https://doi.org/10.9734/BPI/RRAB/V10/2633F","url":null,"abstract":"","PeriodicalId":185276,"journal":{"name":"Recent Research Advances in Biology Vol. 10","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134476892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three Novel Cases of Trilateral Retinoblastoma: An Approach towards Genetic Diagnosis","authors":"R. M. Freitas","doi":"10.9734/BPI/RRAB/V10/9861D","DOIUrl":"https://doi.org/10.9734/BPI/RRAB/V10/9861D","url":null,"abstract":"","PeriodicalId":185276,"journal":{"name":"Recent Research Advances in Biology Vol. 10","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130726938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteasome and Organs Ischemia-Reperfusion Injury: A Review","authors":"J. Oliva","doi":"10.9734/BPI/RRAB/V10/10135D","DOIUrl":"https://doi.org/10.9734/BPI/RRAB/V10/10135D","url":null,"abstract":"","PeriodicalId":185276,"journal":{"name":"Recent Research Advances in Biology Vol. 10","volume":"128 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121898165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Properties of Mesenchymal Stem Cell on Organ Ischemia-reperfusion Injury: A Review","authors":"J. Oliva","doi":"10.9734/bpi/rrab/v10/10134d","DOIUrl":"https://doi.org/10.9734/bpi/rrab/v10/10134d","url":null,"abstract":"The shortage of donor organs is a major global concern. Organ failure requires the transplantation of functional organs. Donor’s organs are preserved for variable periods of warm and cold ischemia time, which requires placing them into a preservation device. Ischemia and reperfusion damage the organs, due to the lack of oxygen during the ischemia step, as well as the oxidative stress during the reperfusion step. Different methodologies are developed to prevent or to diminish the level of injuries. Preservation solutions were first developed to maximize cold static preservation, which includes the addition of several chemical compounds. The next chapter of organ preservation comes with the perfusion machine, where mechanical devices provide continuous flow and oxygenation ex vivo to the organs being preserved. In the addition of inhibitors of mitogen-activated protein kinase and inhibitors of the proteasome, mesenchymal stem cells began being used 13 years ago to prevent or diminish the organ’s injuries. Mesenchymal stem cells (e.g., bone marrow stem cells, adipose derived stem cells and umbilical cord stem cells) have proven to be powerful tools in repairing damaged organs. This review will focus upon the use of some bone marrow stem cells, adipose-derived stem cells and umbilical cord stem cells on preventing or decreasing the injuries due to ischemia-reperfusion.","PeriodicalId":185276,"journal":{"name":"Recent Research Advances in Biology Vol. 10","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127344043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell Death and Organ Injury: The Example of the Kidney","authors":"G. Priante, L. Gianesello, M. Ceol, D. Prete, F. Anglani","doi":"10.9734/BPI/RRAB/V10/9825D","DOIUrl":"https://doi.org/10.9734/BPI/RRAB/V10/9825D","url":null,"abstract":"When tissues are damaged, they usually heal. The cellular responses towards healing require the prior recognition that damage has occurred. Apoptotic cell death is usually a response to the cell’s microenvironment. In fact, prolonged cellular stress may activate homeostatic repair processes, or cells may undergo apoptosis when overwhelmed by the stress. \u0000In the kidney apoptosis contributes to parenchymal cell loss in the course of acute and chronic renal injury, but does not trigger an inflammatory response. On the contrary if inflammation happens, we can have necrosis which differs from apoptosis by the breakdown of integrity of the plasma membrane so necrotic cell death is accompanied by the release of unprocessed intracellular content, including cellular organelles, which are highly immunogenic proteins. The relative contribution of apoptosis and necrosis to injury varies, depending on the severity of the insult. Regulated cell death may result from immunologically silent apoptosis or from immunogenic necrosis. Recent advances have enhanced the most revolutionary concept of regulated necrosis. Several modalities of regulated necrosis have been described, such as necroptosis, ferroptosis, pyroptosis, parhanatos, mitochondria permeability transition regulated necrosis and NETosis. In cell death, mitochondria, which are widely known for their canonical role in cellular respiration and oxidative phosphorylation, are also recognized as key contributors in the cell death pathway, with a central role in detecting and integrating signals from the environment to trigger adaptive and compensatory responses in cells. Thus, mitochondrial damage and dysfunction are identified as one of the pathogenic events in a variety of diseases, including both chronic and acute kidney diseases. Therefore, we review the different modalities of cell death in kidney injury, pointing in particular to converging pathways of cell death and evidencing that a combination therapy targeting multiple cell-death pathways may lead to new opportunities for therapeutic intervention.","PeriodicalId":185276,"journal":{"name":"Recent Research Advances in Biology Vol. 10","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123342709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Genomic Study in Octoploide Triticale (8x) and their Genitors (Wheat and Rye) by C-banding, N-Banding and In Situ Hybridization: The Translocation Identification 2BL/7RS","authors":"Dounia Hammouda, N. Khalfallah, Houda Badri Mohammed","doi":"10.9734/BPI/RRAB/V10/10916D","DOIUrl":"https://doi.org/10.9734/BPI/RRAB/V10/10916D","url":null,"abstract":"Objective: The main objective of this study is to analyze the heterochromatin (DNA sequences non-coding rich in CG base) genomes of a primary triticale, and their progenitors, wheat and rye on the one hand, and on the other to Localizes the nuclear organizer regions (NOR) and ribosomal genes (5S and 45S). \u0000Methodology and Results: The Analysis of C bands of genomes the hybrids are in a comparison with their genitors displayed a different marking. In the genomes A, hybrid (Mahon-demiasxRC9) is less heterochromatic than the genitor D (Mahon-demias). The genomes B and D of the hybrid (Mahon-demias xRC9) show a richness in heterochromatin relative to their homologous of the D genitor. Nevertheless, in R genome of hybrid (Mahondemias xRC9) is almost similar to its E genitor (RC9), except the 2BL / 7RS chromosome. In the genitors and in the hybrids, the organizing regions (NOR) marked on the chromosomes 1R, 2R, 3R and 6R of rye and on the chromosome 1B of wheat and on the chromosomes 1B, 1R, 2R, 3R, 6R of triticale, showed the same localization compared with that is described by authors. The molecular analysis shows that, 5S locus is co-localized with the 45S locus on the chromosome 5R. The 45S loci are located on the 1R, 3R, 5R and 7R chromosomes. They correspond to the nuclear organizer regions (N.O.R). While in triticale, 5S loci are located on chromosomes 2A, 5B and 7RS / 2BL. The 45S locus is located on chromosomes 1R, 3R and 7RS / 2BL. Contrary to the bibliography, the chromosome 5R shows two loci 5S and 45S co localized on the telomer of the short arm. This result suggests that the variety of rye that served as genitor acquired during its selection of rDNA that it transmits to his descendants in a stable manner. 7RS / 2BL Translocation highlighted by C-banding is confirmed by FISH. \u0000Conclusion and Application of Results: The results indicate the existence of intervarietal and interspecific polymorphisms in rye and triticale, as well as the presence of chromosomes B of telocentric form or isochromosomes a heterochromatic and / or euchromatic structure. In a conclusion, we establish a molecular cytogenetic mapping of marker chromosomes and ribosomal genes of our vegetal material (genitors and hybrid).","PeriodicalId":185276,"journal":{"name":"Recent Research Advances in Biology Vol. 10","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116214480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing in vitro Generation of Interleukin-10 Secreting B Regulatory Cells","authors":"K. S. Gupte, A. Vanikar, C. Patel, U. Thakkar","doi":"10.9734/BPI/RRAB/V10/9707D","DOIUrl":"https://doi.org/10.9734/BPI/RRAB/V10/9707D","url":null,"abstract":"A major subset of IL-10 secreting B-cells, commonly characterized as CD19+/38hi/24hi, encompass B-regulatory cells (B-regs). We report effects of changes in stimulating molecule concentration on population of Interleukin-10 secreting B-regs of human origin. B-regs may be obtained from in-vitro co-culture of adipose tissue derived mesenchymal stromal cells (AD-MSC) and peripheral blood mononuclear cells (PBMC) for potential cell therapy. Lipopolysaccharide–E.coli-K12 strain (LPS-EK) was added in varying concentrations in the co-culture of AD-MSC and peripheral blood mononuclear cells (PBMC). At each time point, IL-10 concentration of cell supernatant was estimated by quantitative ELISA. Morphology, sterility, total count, viability and immune-phenotype of cells were determined. We analyzed the data to determine the time interval at which maximum B-reg population and optimum secretion of IL-10 was obtained. The present study provides precious information about the in vitro dynamics of IL-10 secretion by human B-regs generated from AD-MSC and PBMC. The present study focuses on in vitro generation of B-regs from adipose tissue derived mesenchymal stromal cells (AD-MSC) and peripheral blood mononuclear cells (PBMC), as well as on partial characterization of B-regs in terms of amount of IL-10 secreted, time taken and amount of stimulating molecule (i.e. LPS-EK) required for optimum IL-10 secretion. It has been reported that B-regs secrete cytokine in trace quantities. The medium which is added for feeding the cells further dilutes the secreted cytokines by many folds. In our study, the samples were appropriately concentrated, so that the cytokines could be detected by quantitative Enzyme Linked Immunosorbent Assay (ELISA).","PeriodicalId":185276,"journal":{"name":"Recent Research Advances in Biology Vol. 10","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121216499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}