Journal of Biomedical and Translational Research最新文献

Congestive heart failures caused by myxomatous mitral valve degeneration in a Siberian tiger 东北虎二尖瓣黏液瘤变性引起的充血性心力衰竭

Journal of Biomedical and Translational Research Pub Date : 2021-09-01 DOI: 10.12729/jbtr.2021.22.3.141

Jinsung Park, Jong-uk Kim, Jeongho Kim, Gonhyung Kim, B. Ahn

引用次数: 0

Electroacupuncture ameliorates blood-brain barrier dysfunction in 5XFAD Alzheimer’s animal model 电针改善5XFAD阿尔茨海默病动物模型血脑屏障功能障碍

Journal of Biomedical and Translational Research Pub Date : 2021-09-01 DOI: 10.12729/jbtr.2021.22.3.121

Mudan Cai, Jun-Hwan Lee, E. Yang

{"title":"Electroacupuncture ameliorates blood-brain barrier dysfunction in\u0000 5XFAD Alzheimer’s animal model","authors":"Mudan Cai, Jun-Hwan Lee, E. Yang","doi":"10.12729/jbtr.2021.22.3.121","DOIUrl":"https://doi.org/10.12729/jbtr.2021.22.3.121","url":null,"abstract":"Alzheimer’s disease (AD) is an irreversible and progressive neurodegenerative disease accompanied by aging, followed by memory impairment and cognitive decline. Although numerous attempts have been made to develop treatments for AD, most clinical trials have failed to delay or stop the progression of AD. Electroacupuncture (EA) is a complementary alternative medicine technique widely used to treat pain, inflammation, and neurodegenerative diseases. Additionally, blood-brain barrier (BBB) disruption is a known pathophysiology of neurodegenerative diseases, including AD. Moreover, amyloid beta deposition increases BBB permeability and produces inflammatory cytokines induced by glial activation. However, our previous study revealed that EA treatment at the Taegye acupoints (KI3) improves memory impairment through anti-neuroinflammation and increases glucose metabolism in 5XFAD mice. Therefore, we evaluated whether EA treatment at KI3 regulates BBB dysfunction in the prefrontal cortex of 5XFAD mice. For this study, 6.5-month-old 5XFAD mice were treated with EA stimulation at KI3 three times a week for two weeks. Western blotting, immunohistochemistry, and flow cytometry were used to evaluate the effects of EA treatment on BBB dysfunction. We found that EA stimulation attenuates BBB integrity by protecting BBB tight junction proteins (CD31, aquaporin 4, occludin, and claudin 5) in the prefrontal cortex of 5XFAD mice. In addition, EA treatment regulated inflammatory cytokines (IL-1α, IL-1β, IL-17, IL-23, IFN-ɣ, monocyte chemoattractant protein 1 (MCP-1), granulocyte-macrophage colony stimulating factors [GM-CSF], and IL-10) in the peripheral circulation of 5XFAD mice. Therefore, our data suggest that EA treatment could be a therapeutic agent for enhancing BBB dysfunction in AD.","PeriodicalId":173177,"journal":{"name":"Journal of Biomedical and Translational Research","volume":"89 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122953925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reference gene selection for gene expression study in tissues of long-tailed chickens 长尾鸡组织中基因表达研究的内参选择

Journal of Biomedical and Translational Research Pub Date : 2020-12-01 DOI: 10.12729/jbtr.2020.21.4.152

Yunjeong Noh, Woncheoul Park, Han-Ha Chai, D. Lim

{"title":"Reference gene selection for gene expression study in tissues of\u0000 long-tailed chickens","authors":"Yunjeong Noh, Woncheoul Park, Han-Ha Chai, D. Lim","doi":"10.12729/jbtr.2020.21.4.152","DOIUrl":"https://doi.org/10.12729/jbtr.2020.21.4.152","url":null,"abstract":"Quantitative real-time polymerase chain reaction (RTqPCR) is a rapid and precise method of analysis to quantify the level of gene expression and is widely used in the diagnosis of diseases and quantitative analysis of genes. In RT-qPCR analysis, a reference gene (or housekeeping gene) is used for normalization of experimental results. Since this method of analysis detects a small quantity of the product, it is highly sensitive and it is important for the accuracy and reproducibility of the experiment to select a reference gene suitable for gene expression studies. As the expression levels of the reference gene are affected under different conditions, in order to determine the suitability of the housekeeping gene used as the reference gene, it is necessary to verify the expression stability. In the current study, the stability of the expression of 11 housekeeping genes (B2M, SDHA, GAPDH, RPL13, VIM, EEF1A1, HPRT1, GUSB, RPL19, ACTB, and ABL1) was investigated in the tissues of long-tailed chickens (heart, thigh, and breast). Expression stability evaluation was analyzed with four software: BestKeeper, NormFinder, geNorm, and RefFinder. In our study, GAPDH in heart tissue, HPRT1 in thigh tissue, and RPL13 in breast tissue were selected as the most stable reference genes. Evaluation of the expression stability of housekeeping genes can provide important data in gene expression studies by selecting an appropriate reference gene according to various conditions.","PeriodicalId":173177,"journal":{"name":"Journal of Biomedical and Translational Research","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126503869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effects of folic acid on colon carcinogenesis induced by azoxymethane in mice 叶酸对偶氮氧甲烷致小鼠结肠癌的保护作用

Journal of Biomedical and Translational Research Pub Date : 1900-01-01 DOI: 10.12729/jbtr.2021.22.3.111

J. Sohn, Eun Young Kim, Jae-Hwang Jeong, D. Kim, S. Nam, Hyun Jik Lee, B. Lee

{"title":"Protective effects of folic acid on colon carcinogenesis induced by\u0000 azoxymethane in mice","authors":"J. Sohn, Eun Young Kim, Jae-Hwang Jeong, D. Kim, S. Nam, Hyun Jik Lee, B. Lee","doi":"10.12729/jbtr.2021.22.3.111","DOIUrl":"https://doi.org/10.12729/jbtr.2021.22.3.111","url":null,"abstract":"Colon cancer has been considered a leading cause of cancer-associated death. Folic acid is a vitamin necessary for cellular physiological functions and cell viability. However, the association between folic acid intake and colon cancer has been examined in several prospective cohort studies are controversial. This study investigated the effects of folate intake on colon carcinogenesis and oxidative stress in an azoxymethane (AOM)/dextran sodium sulfate (DSS) institute for cancer research (ICR) mouse model. Thirty male ICR mice (5 weeks old) were divided into the control group and the experimental group supplied 0.03% folic acid via drinking water (50 mL/week/mouse) for 6 weeks. To induce colonic pre-neoplastic lesions, the animals were subcutaneously injected three times weekly with AOM (10 mg/kg body weight), followed by 2% DSS in drinking water for a week. Folic acid supplementation significantly suppressed the total number of aberrant crypt foci and aberrant crypts. Histological image data showed that folic acid supplementation attenuated neoplastic change. In addition, we measured the thiobarbituric acid reactive substances concentration of dry feces samples to identify the effect of folic acid on reactive oxygen accumulation. The folic acid supplementation group had reduced reactive oxygen species levels in dry feces compared to the control group. In conclusion, these findings indicate that folic acid suppresses colon carcinogenesis and oxidative stress in an AOM/DSS mouse model.","PeriodicalId":173177,"journal":{"name":"Journal of Biomedical and Translational Research","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133483785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sudden cardiac death as a naturally-occurring ventricular hypertrophy in Macaca fascicularis 心源性猝死是束状猕猴自然发生的心室肥厚

Journal of Biomedical and Translational Research Pub Date : 1900-01-01 DOI: 10.12729/jbtr.2021.22.3.135

Hyeon-Gu Yeo, Junghyung Park, Hyun Kuk Kim, J. Lee, Bon-Sang Koo, Jeong-Hee An, Chang‐Yeop Jeon, You Jung An, Won Seok Choi, Sung-Hyun Park, Jincheol Seo, Jinyoung Won, Keonwoo Kim, J. Cho, Y. Kim, Minji Kim, Jung Bae Seong, J. Huh, Sung Soo Kim, Youngjeon Lee, K. Lim

{"title":"Sudden cardiac death as a naturally-occurring ventricular hypertrophy\u0000 in Macaca fascicularis","authors":"Hyeon-Gu Yeo, Junghyung Park, Hyun Kuk Kim, J. Lee, Bon-Sang Koo, Jeong-Hee An, Chang‐Yeop Jeon, You Jung An, Won Seok Choi, Sung-Hyun Park, Jincheol Seo, Jinyoung Won, Keonwoo Kim, J. Cho, Y. Kim, Minji Kim, Jung Bae Seong, J. Huh, Sung Soo Kim, Youngjeon Lee, K. Lim","doi":"10.12729/jbtr.2021.22.3.135","DOIUrl":"https://doi.org/10.12729/jbtr.2021.22.3.135","url":null,"abstract":"Naturally occurring left ventricular hyperplasia is a rare but lethal disease. There are very few reports of this cardiac disease in captive nonhuman primates. In a colony of Macaca mulatta (Rhesus monkey) at California National Primate Research Center, a large number of rhesus macaques were diagnosed by autopsy with naturally occurring left ventricular hypertrophy without obvious underlying diseases over a 22-year period. The confirmatory diagnosis of ventricular hypertrophy was based on findings of notable left ventricular concentric hypertrophy with reduced left ventricular lumen, which is very similar to human ventricular hypertrophy cases. This report discusses an 11-year-old Macaca fascicularis monkey (Cynomolgus monkey, crab-eating macaque), weighing 2.95 kg, that was presented for enrollment in a pharmacokinetic (PK) study. During the PK experiment, the monkey died following a sudden decrease in percutaneous oxygen saturation and heart rate. Gross and histological examinations of the heart were performed. On gross pathology, the left ventricular wall was thickened, and the chamber lumen was reduced. In histopathological examination using hematoxylin-eosin and Masson-trichrome stains, fibrosis and myocyte disarray were not observed, but an increased cell density, compared to the normal heart, was confirmed. The autopsy results confirmed left ventricular hyperplasia as the major cause of death.","PeriodicalId":173177,"journal":{"name":"Journal of Biomedical and Translational Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129736612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of post-operative C-reactive protein levels between elective general and orthopedic surgery in dogs 犬择期普通手术与骨科手术术后c反应蛋白水平的比较

Journal of Biomedical and Translational Research Pub Date : 1900-01-01 DOI: 10.12729/jbtr.2021.22.3.105

Mukyung Sung, Dongwook Kim, W. Choi, Beomju Bae, Sangui Lee, Yawon Hwang, Kijeng Na, Gonhyung Kim

{"title":"Comparison of post-operative C-reactive protein levels between\u0000 elective general and orthopedic surgery in dogs","authors":"Mukyung Sung, Dongwook Kim, W. Choi, Beomju Bae, Sangui Lee, Yawon Hwang, Kijeng Na, Gonhyung Kim","doi":"10.12729/jbtr.2021.22.3.105","DOIUrl":"https://doi.org/10.12729/jbtr.2021.22.3.105","url":null,"abstract":"C-reactive protein (CRP) is an acute-phase protein produced by hepatocytes. The plasma concentration of CRP peaks around 48 hours following stimulation. A rapid decrease in CRP levels is observed when the stimuli end. Considering these characteristics of CRP, it is used to evaluate acute inflammatory responses in clinics. In addition, as it reflects the degree of surgical trauma, measuring the CRP concentration is an effective method of evaluating tissue damage depending upon the surgical method. As CRP is also an acute-phase protein in dogs, the purpose of the present study was to compare the factors related to acute inflammatory responses between dogs undergoing elective general surgery (GS) and orthopedic surgery (OS). A total of 44 healthy dogs were included in this study. The sex ratio of dogs that underwent OS was similar. Both groups showed increased CRP levels and white blood cell (WBC) counts on post-operative day (POD) 1-2. The CRP level in the OS group on POD 1-2 was significantly higher than that of the GS group and it was increased about 16-fold compared to the pre-operative value. No significant difference in WBC counts was observed between the two groups. Although a slight increase in the WBC count was observed on POD 1-2 in both groups, it was near the reference range during follow-up. Thus, measuring CRP levels can be more effective in evaluating acute inflammatory responses than WBC counts. Although a high level of post-operative serum CRP concentrations was observed in the OS group, it rapidly decreased to a level similar to the pre-operative value if there were no post-operative complications.","PeriodicalId":173177,"journal":{"name":"Journal of Biomedical and Translational Research","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124134105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0