发布求助
文献互助 智能选刊 最新文献

Single Molecule Spectroscopy and Superresolution Imaging XIV最新文献

筛选
英文 中文
Exchangeable fluorophore labels in super-resolution fluorescence microscopy 超分辨荧光显微镜中可交换荧光团标记
Single Molecule Spectroscopy and Superresolution Imaging XIV Pub Date : 2021-03-05 DOI: 10.1117/12.2584988
M. Heilemann
{"title":"Exchangeable fluorophore labels in super-resolution fluorescence microscopy","authors":"M. Heilemann","doi":"10.1117/12.2584988","DOIUrl":"https://doi.org/10.1117/12.2584988","url":null,"abstract":"We present fluorophore labels that transiently and repetitively bind to their targets as probes for various types of super-resolution fluorescence microscopy. Transient labels typically show a weak affinity to a target, and exchange constantly with the buffer that constitutes a reservoir with a large amount of intact probes, leading to repetitive binding events to the same target (we refer to these labels as “exchangeable labels”). This dynamic labeling approach is insensitive to common photobleaching and yields a constant fluorescence signal over time, which has been successfully exploited in SMLM, STED, single-particle tracking and super-resolution optical fluctuation imaging (SOFI). We discuss properties of suitable exchangeable labels and experimental parameters for optimal performance for the different super-resolution methods. In addition, we show how to combine different classes of exchangeable labels for high-quality multicolor super-resolution imaging.","PeriodicalId":167087,"journal":{"name":"Single Molecule Spectroscopy and Superresolution Imaging XIV","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114270039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Front Matter: Volume 11650 前题:卷11650
Single Molecule Spectroscopy and Superresolution Imaging XIV Pub Date : 1900-01-01 DOI: 10.1117/12.2596577
{"title":"Front Matter: Volume 11650","authors":"","doi":"10.1117/12.2596577","DOIUrl":"https://doi.org/10.1117/12.2596577","url":null,"abstract":"","PeriodicalId":167087,"journal":{"name":"Single Molecule Spectroscopy and Superresolution Imaging XIV","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125759425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信