International Journal of Endocrinology: Diabetes and Metabolism最新文献

{"title":"Body Fat Removal","authors":"Michael V. Tyurin","doi":"10.55124/ijde.v1i1.150","DOIUrl":"https://doi.org/10.55124/ijde.v1i1.150","url":null,"abstract":"Food fat gets distributed in the blood and therefore may be removed from the blood.  Fat removal is simple and efficient using the blood circulation through the blood fat removal column described herein. The resulting body weight after 20 blood fat removal procedures might be 230 lbs.  The patient has reached her body size fitting the suits purchased before the weight accumulation","PeriodicalId":163207,"journal":{"name":"International Journal of Endocrinology: Diabetes and Metabolism","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115486026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rejection of vaccination against the COVID-19 virus will increase morbidity and mortality","authors":"Mohammadreza Rezaeipour","doi":"10.55124/ijde.v1i1.134","DOIUrl":"https://doi.org/10.55124/ijde.v1i1.134","url":null,"abstract":"It has been about two years since the first COVID-19 epidemic began,now it coincides with the fifth peak of epidemic infection in some countries. Over the years we have experienced many ups and downs of this virus(1, 2).\u0000It has been about two years since the first COVID-19 epidemic began,now it coincides with the fifth peak of epidemic infection in some countries. Over the years we have experienced many ups and downs of this virus(1, 2). Some people are still with us, but unfortunately, some have left us in the middle of their lives, and they have become victims of the COVID-19 pandemic(3). A virus that when it got into the world of people, many said: \"It does not last long and when it reaching warm seasons of the year, it will die prematurely and does not survive.\" However, the virus has survived to this today.\u0000Efforts have been made around the world to shine the bright rays of hope and life in the shady cornerscreated by it(4, 5). One of these constructive efforts is to obtain a vaccine against COVID-19.  COVID-19 vaccination rates have still not been optimal in some countries(6, 7). While China, India, and the United States have had the highest vaccination rates (over 100%) since August 4, 2021, the statistics are disappointing in some other countries(8). For example, approximately 1.16% of Iranians received vaccinations at the same time(6-8). This is not sufficient to achieve so-called herd immunity, and hospitals have become more crowded due to the serious spread of the more contagious type of delta.\u0000In addition to the lack of appropriate vaccines due to economic sanctions following the political tensions in some countries,such as Iran(9, 10), the reasons for refusing free injections and neglecting preventive measures are also\u0000different(5). The covid-19 virus survived and mutant and increased mortality. However, still, some people don't hear and look at these facts. The efforts of medical staff across the globe have not yet been properly heard(5). What remains are the challenges that have endangered human life on earth, and we seem to be counting it down. These barriers must be identified in a short time. The workload of medical staff has grown significantly. In addition, journalists and the press have come to their aid, reporting and promoting the views of professors, experts, and heads of the health care system in the press, and enlightenment efforts have reached the point where politicians are talking about COVID-19 in election campaigns(11). Although an unknown and difficult path has been taken so far, it seems that this path is still ahead(7). In addition to government agencies, removing these barriers requires residents' campaigns to get vaccinated for their health, for their grandparents, for their neighbors, or to use free incentives(5, 12).\u0000Failure to do so will result in significant health costs for governments and economies.Failure to get COVID-19 vaccination in most cases leads to hospitalization and usually leads to large bills.","PeriodicalId":163207,"journal":{"name":"International Journal of Endocrinology: Diabetes and Metabolism","volume":"145 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115906719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural Competence for Transformation in Lactobacili","authors":"M. Tyurin","doi":"10.55124/ijde.v1i1.119","DOIUrl":"https://doi.org/10.55124/ijde.v1i1.119","url":null,"abstract":"We have detected the changes in the molecular biologic properties of the lascobacilus strain Lactibacillus buchneri NRRLB1837 after its storage in the lyophilized state using some patented lyophilization media.  The change noticed was the 3 orders of magnitude decrease of the natural competence for the genetic transformation capability of said lac-tobacillus strain.  Using noted difference we have transformed L. buchneri NRRLB1837 with the largest plasmid of Lactobacillus casei MT205, possessing strong antibacterial activity against gram-positive, gram-negative bacteria and Candida albicans.  The trans formants became resistant to Erythromycin (30 mcg/ml) and also had strong antibacterial activity, just like L. casei MT205, which was extractable from the concentrated by the evaporation cultural fluid of the respective transformants, The role of the lyophilization medium on the molecular biological properties of the lactobacilli stored lyophilized is dis-cussed. \u0000Introduction \u0000We have noticed about three orders in magnitude difference in the natural transfroma-tion capability in the strain of Lactobacillus buchneri NRRLB1837 depending on the par-ticular conditions of its lyophilization.  Said difference is esssential, since sometimes the natural capability to uptake the foreign plasmid DNA gets below the threshold of de-tection and may not be noticed.  We have detected, that for the strain of Lactobacillus buchneri NRRLB1837 such activity is the highest, if the lyophilization was performed using standard lyophilization medium comprising only sucrose and gelatin, but not the 0.1 M Trtis (pH8.0) and 10% adonit [1]. Routinely, deep freezers (-80oC) are used for the prolonged storage of the microbial cultures under the normal room temperatures.  However, most of the shipments are performed with the microbial cultures, lyophilized for the long-term storage.  We have noticed significant difference in the natural transforma-tion capability of Lactobacillus buchneri NRRLB1837 depending on the composition of the medium for its freeze drying. In particular, we noticed significant decrease of the natural genetic transformation capability in Lactobacillus buchneri NRRLB1837 depend-ing on the compositon of the medium for its freeze drying.  The decrease of that capabil-ity by three orders of magnitude was observed for the freeze dryed culture of said lacto- \u0000  \u0000bacilli freeze dryed in our patented medium for the freeze drying [1].  The core of our patent was the neutralization of the organic acids produced enormously by the intestinal lactobacilli by the 0.1 M Tris buffer (pH8.0) and the lyophilization viability was substan-tially (by 90%) increased when we added some cryoprotective sugar up to 10 vol % to said 0.1 M Tris buffer (autoclaved at 120oC for 30 min).  Today we have faced the fact, that the natural competence of the plasmid DNA uptake reported by us earlier [2] was accompanied with the decreased frequency of the genetic transfer for the plasmid DNA ","PeriodicalId":163207,"journal":{"name":"International Journal of Endocrinology: Diabetes and Metabolism","volume":"1202 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124793818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression in situ of the recombinant human erythropoetin and recombinant insulin.","authors":"Michael V. Tyurin","doi":"10.55124/ijde.v1i1.135","DOIUrl":"https://doi.org/10.55124/ijde.v1i1.135","url":null,"abstract":"","PeriodicalId":163207,"journal":{"name":"International Journal of Endocrinology: Diabetes and Metabolism","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123943632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}