国际药学研究杂志最新文献

{"title":"Evaluation of 10 kits for antibody detection of SARS-CoV-2","authors":"Wei Liu, Chaojie Yang, Liang Qu, Y. Ke, Yong Chen, R. Jia, Xiong Liu, L. Jia, Yong Wang, Li Han, G. Chang, Changjun Wang","doi":"10.13220/J.CNKI.JIPR.2020.09.006","DOIUrl":"https://doi.org/10.13220/J.CNKI.JIPR.2020.09.006","url":null,"abstract":"Objective: To provide data reference for the clinical diagnosis of COVID-19, promote the innovation and improvement of antibody detection technology of COVID-19, and discuss the application of antibody detection of new coronavirus by evaluating the sensitivity, specificity, compliance rate and limit of antibody detection in 10 SARS-CoV-2 antibody detection kits Methods: According to the instructions of 10 new coronavirus antibody detection kits, the specific IgM and IgG in 74 serum samples were detect- ed, and the limit of detection of each kit was tested with 10 samples diluted by a 2-fold gradient Results: The coincidence rate of 10 kits(product A-product J)were 89 2%(66/74), 89 2%(66/74), 86 5%(64/74), 95 9%(71/74), 52 7%(39/74), 75 7%(56/74), 86 5%(64/74), 79 7%(59/74), 50 0%(37/74), 20 3%(15/74), respectively In the detection limit tests for the kits A-J with the 10 of 2x gradient diluted samples, the performance of kit C was the best, with the limit of detection for IgM 0 16AU/ml and the limit of detection for IgG 1 00 AU/ml Conclusion: There are significant differences in sensitivity, specificity, compliance and limit of detection of 10 new coronavirus antibody testing reagents, and the product of high sensitivity, specificity, and compliance rates should be selected for clinical applications to ensure the accuracy of test results","PeriodicalId":16265,"journal":{"name":"Journal of International Pharmaceutical Research","volume":"47 1","pages":"716-721"},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48435523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of nucleic acid detection kits and nucleic acid extraction methods for SARS-CoV-2 using clinical samples","authors":"C. Yang, W. Liu, L. Qu, Y. Ke, Y. Chen, R. Jia, X. Liu, Y. Wang, L. Han, L. Jia, C. J. Wang","doi":"10.13220/J.CNKI.JIPR.2020.06.004","DOIUrl":"https://doi.org/10.13220/J.CNKI.JIPR.2020.06.004","url":null,"abstract":"Objective: To compare and analyze 7 new coronavirus nucleic acid detection kits and 5 nucleic acid extraction methods Methods: After extracting nucleic acids from 44 positive coronavirus clinical samples, 7 SARS-CoV-2 nucleic acid detection kits were used for RT-PCR amplification experiments to compare the positive rate and Ct value;33 new coronavirus positive clinical samples were selected to compare the acid extraction methods Five different nucleic acid extraction methods were used to extract the samples, and then RT-PCR amplification experiments were performed to compare the positive rate and Ct value Results: The brand A nucleic acid extraction kit had the highest positive rate and the lowest rate of missed detection;comparison of nucleic acid extraction methods showed that the manual column extraction method had the highest positive rate, followed by the magnetic bead extraction method, and the one-step extraction method had the highest missed detection rate Conclusion: The detection capabilities of the SARS-CoV-2 detection kits are uneven, so evaluation work needs to be done before the selection of the kit The manual column extraction method showed best extraction efficiency but took a long time Because of the possible combination with the automatic nucleic acid extraction instrument, the magnetic bead extraction method had a high extraction efficacy, which might be suitable for use in the ex- traction of large batches of samples Although the one-step extraction method was easily operable, the method had a high missed detection rate, so this method was not recommended for clinical use","PeriodicalId":16265,"journal":{"name":"Journal of International Pharmaceutical Research","volume":"47 1","pages":"424-429"},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45449387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic effects and possible mechanisms of sirolimus on nephritic mice with chronic rejection","authors":"M. Zheng, C. Hou, R. Wang, Yingying Wei, Jilu Zhang, Xin Li, Guojiang Chen, G. Han, B. Shen, Yan Li, Yuanfang Ma, He Xiao","doi":"10.13220/J.CNKI.JIPR.2014.03.014","DOIUrl":"https://doi.org/10.13220/J.CNKI.JIPR.2014.03.014","url":null,"abstract":"Objective To explore the therapeutic effects of sirolimus on nephritic mice with chronic rejection,and the possible mechanisms. Methods B6D2F1mice transplanted with cell mixture of spleen,thymus,and lymph nodes from DBA /2 spleen,thymus,and lymph nodes cell mixture were used to construct murine model with chronic rejection,and the transplanted mice were randomly divided into two groups. Mice in the sirolimus-treated group were given sirolimus orally in a dose of 1 mg /( kg·d) for 12 consecutive weeks,while mice in the control group were administered with equal amounts of olive oil. Nephritis in mice was confirmed by urine protein determination and histopathological analysis. Enzyme immunoassay was used to detect the serum levels of anti-DNA antibodies including anti-ds DNA IgG,anti-ds DNA IgG1,anti-ds DNA IgG2a,anti-ss DNA IgG,anti-ss DNA IgG1,and anti-ss DNA IgG 2a. Real-time PCR analysis was conducted to evaluate the gene expression of interleukin-6( IL-6),tumor necrosis factor-α( TNF-α),interferon-γ( IFN-γ),interleukin-1β( IL-1β),monocyte chemoattractant protein 1( MCP-1),regulated upon activation normal T cell expressed and secreted( RANTES),B lymphocyte chemoattractant( BLC),transforming growth factor β1( TGF-β1),and collgen I. The proportion and activation of T and B lymphocytes in peripheral blood or spleen were determined by flow cytometric analysis.Results At the end of observation period,the incidence of nephritis in mice treated with sirolimus was significantly lower than that of mice treated with olive oil( P 0. 05). Histopathological evaluation of kidney specimen showed evident vascular intimal hyperplasia and mononuclear cell infiltration in control group. In contrast,no obvious kidney lesions was observed in sirolimus-treated mice. Furthermore,the levels of anti-DNA antibodies and the transcriptional expression of lupus IL-6,TNF-α,IFN-γ,IL-1β,MCP-1,RANTES,BLC,TGF-β1,and collgen I were significantly down-regulated in sirolimus-treated group as compared with control group. Flow cytometric analysis indicated that both of the proportion of activated /memory T cells in peripheral blood and the expression level of surface activation markers on T and B lymphocytes in spleen were significantly decreased in mice exposed to sirolimus as compared with those exposed to olive oil( P 0. 05). On the other hand,the proportion of CD4+FOXP3+regulatory T cells in spleen was significantly upregulated in sirolimus-treated group as compared with control group. Conclusion Sirolimus may inhibit the proliferation and activation of effector cells and downregulate the expression of chemokines and inflammatory factors through up-regulation of CD4+FOXP3+T cells,thus effectively ameliorating the progression of nephritis in mice.","PeriodicalId":16265,"journal":{"name":"Journal of International Pharmaceutical Research","volume":"41 1","pages":"335-341"},"PeriodicalIF":0.0,"publicationDate":"2014-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66256763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}