Journal of Hunan Normal University最新文献

筛选
英文 中文
Effects of glucose on apoptosis of endothelial progenitor cells from human peripheral blood 葡萄糖对人外周血内皮祖细胞凋亡的影响
Journal of Hunan Normal University Pub Date : 2010-12-10 DOI: 10.3760/CMA.J.ISSN.1008-1372.2010.12.012
Liu Ze-hao
{"title":"Effects of glucose on apoptosis of endothelial progenitor cells from human peripheral blood","authors":"Liu Ze-hao","doi":"10.3760/CMA.J.ISSN.1008-1372.2010.12.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1008-1372.2010.12.012","url":null,"abstract":"Objectives To investigate the effects of glucose on apoptosis rate of cultured endothelial progenitor cells (cEPCs). Methods The peripheral blood of healthy adults was isolated by density gradient centrifugation, and mononuclear cells (MNCs) were inducted to differentiate at cultured conditions.EPCs were identified by Dil-acLDL and FITC-UEA-1 as double fluorescent-positive cells. The effectsof glucose at different concentrations on apoptosis rate of the harvested EPCs were measured by fluorescent microscope and fluorescence-activated cell sorting (FACS) after staining with Annexin V-FITC and PI. Results No significant differences were observed in apoptosis rate between samples treatedwith 5.6mmol/l glucose and 11. 1 mmol/l. P >0. 05). 25.5 mmol/L glucose enhanced the EPCsapoptosis rate in a time-dependent manner( P <0. 05). Conclusion High concentration glucose can accelerate apoptosis rate of EPCs in a time-dependent manner. \u0000 \u0000Key words: \u0000Glucose/PD;  Endothelial cells/DE/PA;  Apoptosis/DE","PeriodicalId":16033,"journal":{"name":"Journal of Hunan Normal University","volume":"55 1","pages":"1620-1622"},"PeriodicalIF":0.0,"publicationDate":"2010-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73821609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信