{"title":"Superhydrophobic Nickel Films Fabricated by Electro/Electroless Deposition","authors":"M. Da-li","doi":"10.1109/icept.2011.6066815","DOIUrl":"https://doi.org/10.1109/icept.2011.6066815","url":null,"abstract":"Superhydrophobic nickel films were prepared by electrodeposition and electroless deposition without chemical modification.Experimental results reveal that the nickel film fabricated by electrodeposition is characterized with nanocone arrays and only has a contact angle of about 112°.By coupling electroless deposition,as the second step,hemispherically topped nanocones are generated when electroless deposition time amounts 1min and a high contact angle as much as 152.3° is obtained;nickel surface has successfully transformed from hydrophobic to superhydrophobic.","PeriodicalId":15940,"journal":{"name":"Journal of Fudan University","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82178573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rui-liang Jin, R. Liu, Hong Wang, Jian-wen Cao, Shengfeng Xu, L. Chunyu, Y. Xu, H. Wang
{"title":"Cloning and expression of the histidinol dehydrogenase gene from mycobacterium tuberculosis H37Rv and properties of the recombinant histidinol dehydrogenase","authors":"Rui-liang Jin, R. Liu, Hong Wang, Jian-wen Cao, Shengfeng Xu, L. Chunyu, Y. Xu, H. Wang","doi":"10.1142/9789812835673_0001","DOIUrl":"https://doi.org/10.1142/9789812835673_0001","url":null,"abstract":"The Mycobacterium tuberculosis hisD gene encoded histidinol dehydrogenase(MtHDH) was amplified by PCR from Mycobacterium tuberculosis H37Rv strain genomic DNA and cloned into a prokaryotic expression vector pET-28a(+) to construct the recombinant expression plasmid pET-28a-HDH.Then this recombinant plasmid was transformed into the strain E.coli BL 21(DE3) and highly expressed after induction with IPTG.Purified MtHDH can catalyse L-histidinol to the corresponding amino acid L-histidine.The optimal pH and temperature of the MtHDH were 8.3 and 45 ℃,respectively.The specific activity of MtHDH was 1.788 U/mg and the relative activity was promoted in the presence of Mn2+,Ca2+,Zn2+ and Co2+.The kinetic constants was determined: Km for NAD+ was found to be 0.9765 mmol/L and for histidinol 2.755 μmol/L.Circular dichroism studies on the MtHDH indicated that the secondary structure of the recombinant protein had about 20.5% α-helix,40.9%β-sheet,4.2%β-turn,34.3% random coil at 25 ℃.","PeriodicalId":15940,"journal":{"name":"Journal of Fudan University","volume":"89 1","pages":"21-30"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80226387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Progress on the Electrolytes for Dye-sensitized Solar Cells","authors":"Lin Jian-ming","doi":"10.1515/energyo.0072.00023","DOIUrl":"https://doi.org/10.1515/energyo.0072.00023","url":null,"abstract":"","PeriodicalId":15940,"journal":{"name":"Journal of Fudan University","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80363257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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