Journal of Biomaterials and Nanobiotechnology最新文献

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Transparent Electrode Materials for Simultaneous Amperometric Detection of Exocytosis and Fluorescence Microscopy. 同时用于胞吐和荧光显微镜安培检测的透明电极材料。
Journal of Biomaterials and Nanobiotechnology Pub Date : 2012-01-01 DOI: 10.4236/jbnb.2012.322030
Kassandra Kisler, Brian N Kim, Xin Liu, Khajak Berberian, Qinghua Fang, Cherian J Mathai, Shubhra Gangopadhyay, Kevin D Gillis, Manfred Lindau
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引用次数: 41
MEASUREMENT of Protein 53 Diffusion Coefficient in Live HeLa Cells Using Raster Image Correlation Spectroscopy (RICS). 用光栅图像相关光谱(RICS)测定活HeLa细胞中蛋白53的扩散系数。
Journal of Biomaterials and Nanobiotechnology Pub Date : 2010-10-01 DOI: 10.4236/jbnb.2010.11004
Sungmin Hong, Ying-Nai Wang, Hirohito Yamaguchi, Harinibytaraya Sreenivasappa, Chao-Kai Chou, Pei-Hsiang Tsou, Mien-Chie Hung, Jun Kameoka
{"title":"MEASUREMENT of Protein 53 Diffusion Coefficient in Live HeLa Cells Using Raster Image Correlation Spectroscopy (RICS).","authors":"Sungmin Hong,&nbsp;Ying-Nai Wang,&nbsp;Hirohito Yamaguchi,&nbsp;Harinibytaraya Sreenivasappa,&nbsp;Chao-Kai Chou,&nbsp;Pei-Hsiang Tsou,&nbsp;Mien-Chie Hung,&nbsp;Jun Kameoka","doi":"10.4236/jbnb.2010.11004","DOIUrl":"https://doi.org/10.4236/jbnb.2010.11004","url":null,"abstract":"<p><p>We have applied Raster Image Correlation Spectroscopy (RICS) technique to characterize the dynamics of protein 53 (p53) in living cells before and after the treatment with DNA damaging agents. HeLa cells expressing Green Fluorescent Protein (GFP) tagged p53 were incubated with and without DNA damaging agents, cisplatin or eptoposide, which are widely used as chemotherapeutic drugs. Then, the diffusion coefficient of GFP-p53 was determined by RICS and it was significantly reduced after the drug treatment while that of the one without drug treatment was not. It is suggested that the drugs induced the interaction of p53 with either other proteins or DNA. Together, our results demonstrated that RICS is able to detect the protein dynamics which may be associated with protein-protein or protein-DNA interactions in living cells and it may be useful for the drug screening.</p>","PeriodicalId":15077,"journal":{"name":"Journal of Biomaterials and Nanobiotechnology","volume":"1 1","pages":"31-36"},"PeriodicalIF":0.0,"publicationDate":"2010-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3146256/pdf/nihms281029.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30045251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
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