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Mutagenicity of selected polycyclic aromatic hydrocarbons (PAHs) 部分多环芳烃(PAHs)的致突变性

Journal of Cell Biology and Genetics Pub Date : 2023-06-30 DOI: 10.5897/jcbg2023.0053

E. Awulu

{"title":"Mutagenicity of selected polycyclic aromatic hydrocarbons (PAHs)","authors":"E. Awulu","doi":"10.5897/jcbg2023.0053","DOIUrl":"https://doi.org/10.5897/jcbg2023.0053","url":null,"abstract":"Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants with a high octanol-water partition coefficient ( kow ) and are persistent in the environment. PAHs have various modes of action including mutagenicity, carcinogenicity, and teratogenicity. The mutagenic sensitivity to PAHs of single-cell gel electrophoresis (Comet assay) was compared with the Ames reversion Salmonella experiment (using Salmonella typhimurium TA98, TA100 and TA102) were with and without an exogenous metabolic activation system (S9 mix). S. typhimurium strains TA98, TA100 and TA102 treated with 4 and 40 µM of benzo[a]pyrene, 2-methylnaphthalene, and phenazine with and without S9 mix. Similarly, Caco-2 cells were treated with 5, 10, 20 and 40 µM of the chosen PAHs in the presence or absence of S9 mix. Even at the lowest treatment concentration (4 µM), benzo[a]pyrene, 2-methylnaphthalene and phenazine, significantly ( p < 0.05 ) increased the number of revertant colonies of S. typhimurium TA98, TA100, and TA102 with S9 mix only. Similarly, the chosen PAHs significantly ( p < 0.05 ) increased the tail moments of Caco-2 cells at the lowest treatment concentration (5 µM), resulting in decreased cell growth and viability as in the case of 2-methylnaphthalene. However, DNA damage to Caco-2 cells was not dependent on the S9 mix. The comet assay exhibits a comparable and more sensitive reaction to the tested PAHs than the Ames assay due to the inherent CYP450 metabolic pathway in mammalian cells.","PeriodicalId":147851,"journal":{"name":"Journal of Cell Biology and Genetics","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126771208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterisation of induced mutagenicity via single-cell gel electrophoresis in RAW264.7 and Caco-2 cells by hydrogen peroxide 过氧化氢诱导RAW264.7和Caco-2细胞诱变的单细胞凝胶电泳特性

Journal of Cell Biology and Genetics Pub Date : 2022-02-28 DOI: 10.5897/jcbg2021.0051

E. Awulu

{"title":"Characterisation of induced mutagenicity via single-cell gel electrophoresis in RAW264.7 and Caco-2 cells by hydrogen peroxide","authors":"E. Awulu","doi":"10.5897/jcbg2021.0051","DOIUrl":"https://doi.org/10.5897/jcbg2021.0051","url":null,"abstract":"The need for a complementary short-term mutagenicity bioassay with robust endpoints to the Ames assay has become increasingly crucial to in order to avoid false negative results. The alternative shortterm test (STT) used in conjunction with the Ames increases the validity and decreases the number of false positive outcomes. As a result, Caco-2 cells (Human intestinal epithelial cell model) and RAW264.7 cells (mouse microphage-like cell line) were treated for 24 h with graded doses of hydrogen peroxide (0, 5, 10, 20, and 40 μM) (oxidative stress-inducing mutagen). Singleand double-strand DNA damage was quantified using single-cell gel electrophoresis (Comet assay). The head intensity, tail intensity, tail migration, and tail moment of the damaged DNA were analysed using an epifluorescence microscope with a gated camera and installed comet IV image analysis software. In Caco-2 and RAW264.7 cells, a significant drop in head intensity and a corresponding dose-dependent increase in tail intensity, tail migration, and tail moment are seen when were quantified w compared to the solvent control. The single cell gel electrophoresis (Comet assay) is a very sensitive, robust, and statistically reliable method for determining DNA damage utilising many parameters. As such, the comet assay is advised as a complement to existing short-term bioassays for mutagenicity, such as the Ames assay.","PeriodicalId":147851,"journal":{"name":"Journal of Cell Biology and Genetics","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126953971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0