Cory Sylber, J. Petree, K. Salaita, C. Wongtrakool
{"title":"Scavenger Receptor-Mediated Uptake of DNAZyme-Gold Nanoparticle Conjugates in Alveolar Macrophages Using Two Photon Microscopy Analysis","authors":"Cory Sylber, J. Petree, K. Salaita, C. Wongtrakool","doi":"10.1164/ajrccm-conference.2020.201.1_meetingabstracts.a2293","DOIUrl":"https://doi.org/10.1164/ajrccm-conference.2020.201.1_meetingabstracts.a2293","url":null,"abstract":"","PeriodicalId":145462,"journal":{"name":"A64. THERAPEUTICS, DRUG DELIVERY, AND TISSUE ENGINEERING","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132412503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Belhaj, L. Dewachter, K. Entee, R. Naeije, B. Rondelet
{"title":"Effects of Tacrolimus on Mechanical and Humoral Determinants of Brain Death-Induced Lung Injury in Piglets","authors":"A. Belhaj, L. Dewachter, K. Entee, R. Naeije, B. Rondelet","doi":"10.1183/13993003.congress-2019.pa3353","DOIUrl":"https://doi.org/10.1183/13993003.congress-2019.pa3353","url":null,"abstract":"The mechanisms of brain death (BD)-induced lung injury is due to mechanical and humoral perturbations. Brain death was induced by slow intracranial blood infusion in anesthetized pigs after randomization to placebo (n = 9) or to tacrolimus (n = 8 ; 0.1 mg/Kg/J) to inhibit the expression of pro-inflammatory mediators. Pulmonary artery pressure (PAP), wedged PAP (PAWP), pulmonary vascular resistance (PVR) and effective pulmonary capillary pressure (PCP) were measured 2, 4 and 6 hours after Cushing reflex. Lung tissue was sampled to determine gene expressions of cytokinesand pathologically score lung injury. Intracranial hypertension caused a transient increase in blood pressure followed, after brain death was diagnosed, by persistent increases in PAP, PCP and the venous component of PVR, while PAWP did not change. Arterial PO2/fraction of inspired O2 (PaO2/FiO2) decreased. Brain death was associated with an accumulation of neutrophils in lung tissue together with increased pro-inflammatory interleukin (IL)-6/IL-10 ratio. Blood expressions of IL-6 and IL-1β were also increased. Blood expressions of IL-6 and IL-1β were also increased. Tacrolimus pre-treatment was associated with a blunting of increased PCP and PVR venous component, which returned to baseline 6 hours after BD, and partially corrected lung tissue biological perturbations. PaO2/FiO2 and lung injury score were prevented. In conclusion, increase in pulmonary capillary pressure with pulmonary venous resistance, associated with lung inflammatory process were partially prevented by tacrolimus BD-induced lung injury pig model.","PeriodicalId":145462,"journal":{"name":"A64. THERAPEUTICS, DRUG DELIVERY, AND TISSUE ENGINEERING","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122405338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}