Biosensors and Bioelectronics Open Access最新文献

Cell Manipulation and Detection Enhancement Enabled by a Microelectromagnet Integrated with a Digital Microfluidic Device 集成了数字微流体装置的微电磁体使细胞操作和检测增强成为可能

Biosensors and Bioelectronics Open Access Pub Date : 2018-11-01 DOI: 10.29011/2577-2260.100045

Liji Chen, A. Madison, R. Fair

{"title":"Cell Manipulation and Detection Enhancement Enabled by a Microelectromagnet Integrated with a Digital Microfluidic Device","authors":"Liji Chen, A. Madison, R. Fair","doi":"10.29011/2577-2260.100045","DOIUrl":"https://doi.org/10.29011/2577-2260.100045","url":null,"abstract":"This work demonstrates sparse cell detection in mL samples, using magnetic bead manipulation on an ElectrowettingOn-Dielectric (EWD) chip. Sparse sample detection was performed in two steps: cell capture off chip from the starting solution with a microelectromagnet and on-chip fluorescent signal detection on an EWD chip. In the first step, immunological reactions enable the binding between target cells and antibody-coated magnetic beads, which enabled sample capture with high cell survival rates. In the second step, fluorescent detection is achieved on an EWD chip via fluorescent signal measurement and two-dimensional magnetic bead concentration. Magnetic bead concentration is controlled with an integrated microelectromagnet, a planar set of half-circle-shaped current-carrying wires embedded in an actuation electrode of an EWD device. This two-dimensional wire structure serves as a microelectromagnet capable of segregating magnetic beads into an area on the order of 10 μm2 with a resulting improvement in Signal-To-Noise Ratio (SNR) of 30 times. Simple device integration ensures that the magnetic bead manipulation and the EWD function can be operated simultaneously without introducing additional steps in the EWD chip fabrication process. Immunological reaction kits were selected in order to ensure the compatibility of target cells, magnetic beads and EWD functions. The magnetic bead choice ensures the binding efficiency and survivability of target cells. The magnetic bead selection and binding mechanism used in this work can be applied to a wide variety of samples with a simple switch of the type of antibody. Sparse cell fluorescent measurements with good SNR are made possible by using fluorescent stains and a method of concentrating cells attached to beads into a small detection area. Theoretical limitation of the entire sparse sample detection system is as low as 1 colony forming unit/mL (CFU/mL). DOI: 10.29011/ 2577-2260. 100045","PeriodicalId":144782,"journal":{"name":"Biosensors and Bioelectronics Open Access","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120964451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Image Quality Evaluation for Video Iris Recognition in the Visible Spectrum 可见光谱下视频虹膜识别的图像质量评价

Biosensors and Bioelectronics Open Access Pub Date : 2018-09-12 DOI: 10.29011/2577-2260.100044

Eduardo Garea-Llano, Dailé Osorio-Roig, Osdel Hernandez-Hernandez

引用次数: 2

Performance Improvement of Hydrogen Sensors in Support of Greening the Future of Energy 提高氢传感器性能，支持未来能源的绿色发展

Biosensors and Bioelectronics Open Access Pub Date : 2018-08-13 DOI: 10.29011/2577-2260.100039

Y. Najjar, S. Mashareh

{"title":"Performance Improvement of Hydrogen Sensors in Support of Greening the Future of Energy","authors":"Y. Najjar, S. Mashareh","doi":"10.29011/2577-2260.100039","DOIUrl":"https://doi.org/10.29011/2577-2260.100039","url":null,"abstract":"Hydrogen has the greatest probability to leak. So, hydrogen detection becomes more challenging than other gases for safety considerations. In this study, Electro-thermal simulation and transient analysis for hydrogen leakage semiconductor sensor were performed. The heater coil and the sensitive layer of the sensor are the main investigated parts. Finite Element Method (FEM) analysis used to compare the electro-thermal properties of two geometrical heater coils and different materials for sensitive layer. The temperature of the simulated sensors was analysed considering the natural convection and radiation, ambient temperature variation from 0-50°C, varying heater coil materials, varying dimensions, and varying sensitive layer materials. Optimization for the heater coil was performed by comparing the maximum surface temperature, power consumption, and the time response for both sensor designs by COMSOL 4.3. The simulated results confirmed that the nickel-chrome material for the heater needs minimum power consumption of 82% lower than Pt. But it has longer response time, which is 37 seconds (240% of Pt response time) at 1V power supply. Also, Comparing SnO2 and ZnO sensitive materials, SnO2 material has a response time lower than ZnO by 28% in helical based sensor and 31% in meander one. Finally, the helical based sensor needs power less than meander one by 40% to achieve the 350°C surface temperature. In other words, helical based sensor generates higher surface temperature by 36% from the meander one at the same power consumption (500mW power supply). DOI: 10.29011/ 2577-2260.100039","PeriodicalId":144782,"journal":{"name":"Biosensors and Bioelectronics Open Access","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131860403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simulation Evaluation of a Label-free Silicon-on-Insulator “Lab on a Chip” Optical Biosensor 无标签绝缘体上硅“片上实验室”光学生物传感器的仿真评估

Biosensors and Bioelectronics Open Access Pub Date : 2018-07-23 DOI: 10.29011/2577-2260.100040

Najala Najeeb, Yaping Zhang, T. Benson

{"title":"Simulation Evaluation of a Label-free Silicon-on-Insulator “Lab on a Chip” Optical Biosensor","authors":"Najala Najeeb, Yaping Zhang, T. Benson","doi":"10.29011/2577-2260.100040","DOIUrl":"https://doi.org/10.29011/2577-2260.100040","url":null,"abstract":"This paper presents the design and optimization of a Silicon-on-Insulator (SOI) “Lab on a Chip” immunosensor, based on interferometer technology, capable of the label-free, real time, parallel detection and identification of multiple analytes with extremely high sensitivity. The basic principles for the biosensor device are evanescent wave sensing and interferometry based on Mach-Zehnder Interferometers (MZIs). For a MZI with a sensing length of 1000μm the theoretical sensitivity is 3.1 × 10-7 Refractive Index Units (RIUs). Sensitivity is further increased by incorporating spiral waveguides to increase the length of the interferometer arms within a given wafer footprint. A spiral of four turns gives a sensing arm length of 3145μm, which takes up an area of 0.06mm2 and gives a theoretical MZI sensitivity of 9.9 × 10-8 RIUs. The sensitivity of detection of the biosensors developed is at least 10-100 times more sensitive than that of current commercial products. Parallel detection is achieved by exciting multiple sensors in parallel using a 1xN Multimode Interferometer (MMI), where N≤12. Y-junction splitters and Directional Couplers (DCs) are evaluated for splitting purposes. A spot-size converter or grating coupler is used to input and output light to and from the optical waveguides. DOI: 10.29011/ 2577-2260.100040","PeriodicalId":144782,"journal":{"name":"Biosensors and Bioelectronics Open Access","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130916945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ssDNA@DAPI/Layered Double Hydroxide Biocompatible Composite Ultrathin Film, Its Assembly and Fluorescence Probe for Telomere Detection ssDNA@DAPI/层状双氢氧化物生物相容性复合超薄膜及其组装及端粒检测荧光探针

Biosensors and Bioelectronics Open Access Pub Date : 2018-07-16 DOI: 10.29011/2577-2260.100038

Yuehua Hu, Ping Zhang, Ruili Ma, Xiaorang Zhang, Jun Lu

{"title":"ssDNA@DAPI/Layered Double Hydroxide Biocompatible Composite Ultrathin Film, Its Assembly and Fluorescence Probe for Telomere Detection","authors":"Yuehua Hu, Ping Zhang, Ruili Ma, Xiaorang Zhang, Jun Lu","doi":"10.29011/2577-2260.100038","DOIUrl":"https://doi.org/10.29011/2577-2260.100038","url":null,"abstract":"Nucleic Acids, the elemental basis of living organisms, play important roles in the life continuation and development, and also are widely used for pathological diagnosis and analysis, therefore the detection of the nucleic acid sequence is very significant in the biological medicial science. Telomere is a special DNA structure at the end of eukaryotic chromosomal that will shorten with age, which is the most important and accurate indicator of person’s aging rate. Therefore, the detection of telomere length is significant for human being. This work describes a systematic study on preparation of Ultrathin Films (UTFs) composite of fluorescent Dye 4’,6-Diamidino-2-Phenylindole (DAPI) blending with Single-Stranded DNA (ssDNA) and Layered Double Hydroxides (LDHs) nanosheets by the layer-by-layer method. This new type of bio-inorganic composite material (ssDNA@DAPI/LDHs)n UTFs show uniform and long-rangeordered periodic layered structure that can be used to detect the complementary ssDNA sequence including the telomere squence with various length. The UTFs exhibits excellent selectivity and reversibility, which has potential applications in the field of bioluminescent sensoring materials. DOI: 10.29011/ 2577-2260.100038","PeriodicalId":144782,"journal":{"name":"Biosensors and Bioelectronics Open Access","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114818001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electronic Biomaterials Towards Flexible Sensors: A Review 面向柔性传感器的电子生物材料研究进展

Biosensors and Bioelectronics Open Access Pub Date : 2018-06-11 DOI: 10.29011/2577-2260.100037

Qingqing Sun, Binbin Qian, K. Uto, Jinzhou Chen, Xuying Liu, T. Minari

引用次数: 3

An Electrochemical DNA Sensor for Ultrasensitive Detection of Aspirin Resistance Gene Based on Signal Amplification Strategy of Ru(NH3)63+/MPA/Cys Redox Cycling 基于Ru(NH3)63+/MPA/Cys氧化还原循环信号放大策略的阿司匹林耐药基因超灵敏检测电化学DNA传感器

Biosensors and Bioelectronics Open Access Pub Date : 2018-06-11 DOI: 10.29011/2577-2260.100036

Yongxian Wan, Lili Xu, N. Zhuo, Xiaobo Lu

{"title":"An Electrochemical DNA Sensor for Ultrasensitive Detection of Aspirin Resistance Gene Based on Signal Amplification Strategy of Ru(NH3)63+/MPA/Cys Redox Cycling","authors":"Yongxian Wan, Lili Xu, N. Zhuo, Xiaobo Lu","doi":"10.29011/2577-2260.100036","DOIUrl":"https://doi.org/10.29011/2577-2260.100036","url":null,"abstract":"Aspirin resistance, the phenomenon of patient showing no response to aspirin, is characterized by a predominant mutation in the COX-1 gene. Accordingly, patients are routinely screened for mutations in this gene to determine whether they can benefit from aspirin treatment. In this work, we reported a simple and ultrasensitive electrochemical method for COX-1 C50T detection for the first time based on Electrochemical-Chemical-Chemical (ECC) redox cycling. In the procedure of assay, Ru(NH3)6 3+ is used as the electron acceptor that is electrostatically attracted to Peptide Nucleic Acid (PNA) modified electrodes and new chemical regents of Mercaptopropionic Acid (MPA) and cysteamine were introduced, which permits Ru(NH3)6 3+ to be regenerated for multiple redox cycles. Different Pulse Voltammetry (DPV) was applied to record the electrochemical signals, which increased linearly with the target DNA. Under optimal conditions, the DNA sensors showed a wide linear relationship, from 10 fM to 1 nM, with detection limits of 2.8 fM (S/N = 3). This strategy paves a new avenue for the determination of aspirin resistance gene in patient serum. DOI: 10.29011/ 2577-2260. 100036.","PeriodicalId":144782,"journal":{"name":"Biosensors and Bioelectronics Open Access","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116993804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smartphone-Enabled Biotelemetric System for A Smart Contact Lens 智能隐形眼镜的智能手机生物遥测系统

Biosensors and Bioelectronics Open Access Pub Date : 2017-04-27 DOI: 10.29011/2577-2260.100001

G. Shaker, Luyao Chen, SafieddinSafaviNaeini

{"title":"Smartphone-Enabled Biotelemetric System for A Smart Contact Lens","authors":"G. Shaker, Luyao Chen, SafieddinSafaviNaeini","doi":"10.29011/2577-2260.100001","DOIUrl":"https://doi.org/10.29011/2577-2260.100001","url":null,"abstract":"Diabetes describes a disordered metabolic state with an overabundance of glucose in the bloodstream, due to insufficient production or utilization of insulin to allow tissue cells from consuming glucose. People with unmanaged diabetes could lead to many serious complications such as heart disease, stroke, coma, kidney failure, blindness, amputation, and premature death. Diabetes can be managed by monitoring the blood glucose level, and control the glucose level by taking insulin, and exercising a carefully planned lifestyle with appropriate diet and physical activities. An elegant solution for glucose monitoring is the integration of electrochemical-based glucose sensor and microelectronics within a contact lens, namely a smart contact lens, which can measure the tear glucose in the eye, and correlate it to blood glucose. Currently, there is no functional smart contact lens devices for glucose detection in the market. This thesis focuses on providing proof of concept prototypes for implementing energy harvesting and wireless data transmission on a smart contact lens. An all-in-one solution is proposed to harvest energy from a smartphone, and use the same smartphone to support glucose data extraction by backscattering. The appropriate prototype architectures are justified based on a system specification estimated from related works. The prototypes are designed in simulation, and then fabricated on PCBs using off-the-shelf components and equipment. Measurements are conducted on the prototypes to evaluate their performance against the initial assessment of requirements from related works.","PeriodicalId":144782,"journal":{"name":"Biosensors and Bioelectronics Open Access","volume":"144 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127560670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection Methods and Tools of Administered Anti-Epileptic Drugs - A Review 给药抗癫痫药物检测方法与工具综述

Biosensors and Bioelectronics Open Access Pub Date : 1900-01-01 DOI: 10.29011/2577-2260.100046

A. Hammoud, D. Nguyen, M. Sawan

{"title":"Detection Methods and Tools of Administered Anti-Epileptic Drugs - A Review","authors":"A. Hammoud, D. Nguyen, M. Sawan","doi":"10.29011/2577-2260.100046","DOIUrl":"https://doi.org/10.29011/2577-2260.100046","url":null,"abstract":"Anti-Epileptic Drugs (AEDs) are prescribed for extended periods of time for patients suffering from disabling seizures. This manuscript reviews main methods and tools available for the detection of AEDs for patients, assessing the advantages and drawbacks of each technique. On one hand, chromatography-based sensors are highly selective and sensitive; however, they are often time-consuming and require specialized technicians and bulky equipment. Optical-based and micro cantilever sensors are sensitive and easy to manipulate; nevertheless, they generally lack selectivity and also require specialized technicians. On the other hand, electrochemical-based sensors, although not as sensitive as chromatography-based ones, are easiest to operate and possess the potential to be selective, miniature, and implantable. After a thorough analysis of the scientific literature, it was found that electrochemical biosensors are most likely the best for Therapeutic Drug Monitoring (TDM) since they are simple, do not require much labor and expertise, are inexpensive, have a quick time of analysis, only require a small sample and can be miniaturized for Point of Care (POC) applications. Moreover, electrochemical sensors may be modified through immobilizing Molecular Imprinted Polymers (MIP) at the Working Electrode’s (WE’s) surface. All these factors make electrochemical techniques most suitable for TDM and remove a lot of the hassle associated with other methods. DOI: 10.29011/ 2577-2260. 100046","PeriodicalId":144782,"journal":{"name":"Biosensors and Bioelectronics Open Access","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117013880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2