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International journal of cell cloning最新文献

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Peripheral blood stem cell autografts. Proceedings of the Second International Symposium. Mulhouse, September 29-October 2, 1991. Abstracts. 自体外周血干细胞移植。第二届国际学术研讨会论文集。米卢斯，1991年9月29日至10月2日。摘要。
International journal of cell cloning Pub Date : 1992-01-01 DOI: 10.1002/stem.5530100763?
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引用次数: 0
Recombinant human erythropoietin corrects anemia of blood loss: a study in the dog. 重组人促红细胞生成素纠正失血性贫血:一项在狗身上的研究。
International journal of cell cloning Pub Date : 1990-03-01 DOI: 10.1002/stem.5530080203?
J Koumegawa, J Kawahara, K Kubo, K Amano, N Wakimoto, K Takahashi, T Juji, F Takaku
{"title":"Recombinant human erythropoietin corrects anemia of blood loss: a study in the dog.","authors":"J Koumegawa,&nbsp;J Kawahara,&nbsp;K Kubo,&nbsp;K Amano,&nbsp;N Wakimoto,&nbsp;K Takahashi,&nbsp;T Juji,&nbsp;F Takaku","doi":"10.1002/stem.5530080203?","DOIUrl":"https://doi.org/10.1002/stem.5530080203?","url":null,"abstract":"<p><p>In order to evaluate the possibility of using recombinant human erythropoietin (rhEpo) for the prevention and correction of anemia due to blood loss and as an adjuvant for autologous blood transfusion, its preventive and therapeutic effects were evaluated in beagles in which anemia was induced by repeated phlebotomies. Two hundred U/kg of rhEpo were administered i.v. four or nine times every two weeks for six weeks. Phlebotomies (25 ml/kg of body weight) were conducted three times at two-week intervals. rhEpo was found to successfully prevent and correct anemia caused by the phlebotomies. Concurrent administration of iron increased efficacy. The findings obtained in the present study suggest that rhEpo is useful both for the treatment of anemia caused by blood loss due to surgery and as an adjuvant therapy for pre-deposit autologous blood transfusion.</p>","PeriodicalId":13742,"journal":{"name":"International journal of cell cloning","volume":"8 2","pages":"97-106"},"PeriodicalIF":0.0,"publicationDate":"1990-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13464276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interference of a human leukocyte interferon preparation with stimulatory activities in leukocyte-conditioned medium for human hematopoietic stem cells. 人白细胞干扰素制剂在白细胞条件培养基中对人造血干细胞具有刺激活性的干扰。
International journal of cell cloning Pub Date : 1984-09-01
L Kanz, G W Löhr, A A Fauser
{"title":"Interference of a human leukocyte interferon preparation with stimulatory activities in leukocyte-conditioned medium for human hematopoietic stem cells.","authors":"L Kanz,&nbsp;G W Löhr,&nbsp;A A Fauser","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Medium conditioned by leukocytes in the presence of phytohemagglutinin (PHA-LCM) promotes the growth of multilineage hemopoietic progenitors derived from human bone marrow. However, PHA-LCM prepared in the presence of a human leukocyte interferon preparation does not support mixed colony formation. Crude PHA-LCM preparations were characterized by gel filtration, affinity chromatography, and gel electrophoresis. The elution profile on Sephacryl S-300 of PHA-LCM prepared without interferon showed a distinct peak that stimulated the growth of pluripotent stem cells (CFU-gemm) and committed precursors (CFU-c, BFU-e). Gel filtration of PHA-LCM, prepared with 1000 U/ml of interferon, revealed a change in the elution profile. The eluted material demonstrated no growth-promoting activities. We conclude that the abolished stimulatory activity of PHA-LCM, prepared with human leukocyte interferon, might be due to a reduced production of stimulatory molecules, suggesting that interferon interferes with the molecular events required for colony formation of committed and noncommitted hemopoietic progenitors.</p>","PeriodicalId":13742,"journal":{"name":"International journal of cell cloning","volume":"2 5","pages":"286-95"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17550679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The establishment of a human myeloma cell line elaborating lambda-light chain protein. 表达lambda-轻链蛋白的人骨髓瘤细胞系的建立。
International journal of cell cloning Pub Date : 1984-05-01
Y Niho, T Shibuya, K Yamasaki, N Kimura
{"title":"The establishment of a human myeloma cell line elaborating lambda-light chain protein.","authors":"Y Niho,&nbsp;T Shibuya,&nbsp;K Yamasaki,&nbsp;N Kimura","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A human myeloma cell line (KMM-56) producing lambda-light chain protein was established in vitro by cultivation of the cells in the pleural effusion obtained from a patient with IgD-lambda-myeloma. The cells proliferate in suspension and do not aggregate or attach to the culture dish. Surface marker analysis revealed that the cells were negative for E-rosette, and surface immunoglobulin. Immunoelectrophoresis, immunodiffusion, and immunofluorescence with various antibodies demonstrated no heavy chains, while lambda-light chains were detected in the cytoplasm of the cells. Using the immunodiffusion technique, only lambda-light chains were detected in the frozen and thawed cell extract, the concentrated supernatant of the cell culture, and the urine of the patient. Electron microscopic examination revealed the plasmablastoid appearance of the cells. This cell line may be useful for future studies of human immunoglobulin genes and for the material of human-human hybridoma, which could produce monoclonal human immunoglobulin.</p>","PeriodicalId":13742,"journal":{"name":"International journal of cell cloning","volume":"2 3","pages":"161-72"},"PeriodicalIF":0.0,"publicationDate":"1984-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17489128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improvement of human melanoma colony formation in soft agar using boiled instead of autoclaved agar. 用煮沸琼脂代替高压灭菌琼脂改善软琼脂中人类黑色素瘤菌落形成。
International journal of cell cloning Pub Date : 1983-06-01
S P Thomson, M D Wright, F L Meyskens
{"title":"Improvement of human melanoma colony formation in soft agar using boiled instead of autoclaved agar.","authors":"S P Thomson,&nbsp;M D Wright,&nbsp;F L Meyskens","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of agar sterilized by either boiling or autoclaving on human melanoma colony formation in soft agar was compared using cells from 17 biopsies of metastatic malignant melanoma. The frequency of colony formation was significantly increased for cells grown in boiled agar in 8 samples (47%), unchanged in 8 samples (47%), and decreased in only one sample (6%). There were increases in both cluster and colony formation for the melanomas which had augmented colony formation when grown in boiled agar. There was also qualitative morphological improvement, including rounder, smoother cells and less extracellular debris surrounding the colonies. These data suggest that melanoma colony formation is enhanced when cells are grown in agar which has been sterilized by boiling rather than autoclaving.</p>","PeriodicalId":13742,"journal":{"name":"International journal of cell cloning","volume":"1 2","pages":"85-91"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17426175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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