{"title":"Negative entropy production in L-lactate dehydrogenase kinetics","authors":"Stepanov Av, Stepanov Ma","doi":"10.17352/ab.000007","DOIUrl":"https://doi.org/10.17352/ab.000007","url":null,"abstract":"Fröhlich theory of coherent excitations in biological systems is analyzed. L-lactate dehydrogenase kinetics is studied using the activation process model for non-equilibrium cases. The model explains the process of energy accumulation necessary for carrying out catalytic reactions in a living cell by means of interaction of quantum subsystems with thermal equilibrium radiation, light, and radiation energy of metabolic processes occurring in the cell. It is shown that lactate dehydrogenase catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in three ways, that is, three reactions occur simultaneously. Negative information entropy for the reactions under consideration is calculated. Its absolute value is equal to that of information entropy of the elementary activation act. This fact ensures the principle of stable non-equilibrium and indicates the reaction under consideration belongs to a living cell.","PeriodicalId":137253,"journal":{"name":"Archive of Biochemistry","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130646161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The use of lntramolecular carbon isotope distributions (13c/12c) of biomolecules to study temporal organization of post-photosynthetic metabolism in a plant cell","authors":"A. Ivlev","doi":"10.17352/ab.000006","DOIUrl":"https://doi.org/10.17352/ab.000006","url":null,"abstract":"","PeriodicalId":137253,"journal":{"name":"Archive of Biochemistry","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124511856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dias Meriellen, de Lacerda José Thalles Jocelino Gomes, Andrade Lidiane Maria, do Nascimento Claudio Augusto Oller, Rozas Enrique Eduardo, Mendes Maria Anita
{"title":"Proteomic analysis of a copper mine isolated fungus Rhizopus microspores IOC 4686 when exposed to copper sulfate","authors":"Dias Meriellen, de Lacerda José Thalles Jocelino Gomes, Andrade Lidiane Maria, do Nascimento Claudio Augusto Oller, Rozas Enrique Eduardo, Mendes Maria Anita","doi":"10.17352/ab.000005","DOIUrl":"https://doi.org/10.17352/ab.000005","url":null,"abstract":"The fungus Rhizopus microsporus, known for its absorption capacity for toxic metals was used to understand the green metal recovery via analysis of its physiology under metal stress conditions through proteomic methods. To investigate the effects of copper stress on fungus, R. microsporus IOC 4686, isolated from the mine environment, was exposed to copper ions (50mgL-1) for 48 h. This study was performed only on copper exposure. Tryptic and chymotryptic extracts of proteins were analyzed by nano- liquid chromatography-tandem mass spectrometry, identification was performed by PEAKS Studio 8.5. Proteins were classified according to their molecular function and biological process. Enzymes, such as catalase, superoxide dismutase, and cytochrome c peroxidase were found in the presence and absence of copper ions. However, only in presence of copper ions, was observed the presence of heat shock proteins (HPS 20, HPS 70, and HPS 78) and metalloproteins (GrpE protein homolog and cytochrome P450). These classes of proteins have been produced by cells in response to stress conditions. The control group (absence of copper ions) also presented antioxidant enzymes suggesting that the fungus isolated from the mine environment already has adapted to the copper. The presence of these proteins suggested a physiological response of R. microsporus IOC 4686 to oxidative stress induced by copper.","PeriodicalId":137253,"journal":{"name":"Archive of Biochemistry","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134086077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A new theoretical basis for description of living matter","authors":"S. Teodorova","doi":"10.17352/AB.000001","DOIUrl":"https://doi.org/10.17352/AB.000001","url":null,"abstract":"In the context of environmental pollution, it is of prime importance to study the organism’s overall response to harmful environments. This could be carried out if there were suitable dynamic variables, describing the state of a living system. In this respect, a new approach to life phenomena is needed because in phenomenological aspect, the biological objects in their entirety could be not adequately described in the terms of other science fields.","PeriodicalId":137253,"journal":{"name":"Archive of Biochemistry","volume":"391 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122080566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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