Kinetics of Enzymatic Synthesis最新文献

Obtaining Enzymatic Extract from Pleurotus spp. Associated with an Integrated Process for Conversion of Lignocellulosic Biomass to Bioproducts 从侧耳菇中提取酶提取物。与木质纤维素生物质转化为生物产品的集成工艺相关

Kinetics of Enzymatic Synthesis Pub Date : 2019-01-30 DOI: 10.5772/INTECHOPEN.79848

A. Serafin-Muñoz, C. Molina–Guerrero, B. N. Luna, Julio César Leal Vaca, A. Á. Vargas

{"title":"Obtaining Enzymatic Extract from Pleurotus spp. Associated with an Integrated Process for Conversion of Lignocellulosic Biomass to Bioproducts","authors":"A. Serafin-Muñoz, C. Molina–Guerrero, B. N. Luna, Julio César Leal Vaca, A. Á. Vargas","doi":"10.5772/INTECHOPEN.79848","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.79848","url":null,"abstract":"The pretreatment of biomass has been integrated with enzyme production through the recycling of aqueous fractions. A process integrated with Pleurotus cystidiosus was grown, and enzymatic hydrolysis was realized. Samples of every liquid fraction from the fungal growing medium were analyzed to determine the chemical oxygen demand (OCD), glucose (Glu), xylose (Xyl), and total reducing sugars (RS). Separately, to obtain valuable polymers from this integration process, solid hemicellulose and lignin were isolated from the remaining liquid fractions through pH variation. The composition of the samples was determined using scanning electron microscopy (SEM), optical stereoscopic microscopy, and Fourier transform infrared (FTIR) spectroscopy and was compared with commercial homologs. The maximum conversion of cellulose to glucose by the obtained liquid fraction of the fungal medium was 61.3 ± 0.9% of the theoretical conversion yield of the commercial enzyme. Similarly, the conversion of hemicelluloses to xylose was 69.5 ± 1.5%. Finally, in this work, an integrated platform for cellulose, hemicellulose, lignin, enzymatic extract, and sugars production, which also significantly reduces water consumption, was proposed. at a of −1 in performed on each","PeriodicalId":136596,"journal":{"name":"Kinetics of Enzymatic Synthesis","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121842576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solvent-Free Isoamyl Acetate Production via Enzymatic Esterification 酶法酯化生产无溶剂醋酸异戊酯

Kinetics of Enzymatic Synthesis Pub Date : 2018-11-20 DOI: 10.5772/INTECHOPEN.81333

N. Azudin, S. R. Shukor

{"title":"Solvent-Free Isoamyl Acetate Production via Enzymatic Esterification","authors":"N. Azudin, S. R. Shukor","doi":"10.5772/INTECHOPEN.81333","DOIUrl":"https://doi.org/10.5772/INTECHOPEN.81333","url":null,"abstract":"Isoamyl acetate is an organic compound which is mainly used as flavor additive in food industries. Traditionally, the food flavor has been produced by extraction from plants, followed by chemical synthesis route which then shifted to biocatalytic route due to consumer ’ s awareness and inclination toward natural products. This study was carried out to examine the reaction synthesis between acetic anhydride and isoamyl alcohol in the presence of Candida antarctica Lipase-B (CALB) as a catalyst in solvent-free system (SFS). Results show that two reactions took place between acetic anhydride and isoamyl alcohol. The effect of different reaction parameters on the final yield of isoamyl acetate and the optimization of process parameters using a statistical tool were also investigated with response surface methodology (RSM). It was found that the optimum isoamyl acetate yield is at reaction temperature 30 (cid:1) C, acid/alcohol molar ratio 0.10, and enzyme loading 4.14%. The regression coefficient for optimization based on RSM was 0.9961. Errors resulted from model validation is less than 1% and is acceptable for real-life application. RSM model and first principle model were selected to determine the reaction kinetics and yield of reaction for isoamyl acetate. The results showed that RSM model provides a good predication of the esterification system with R 2 value of 0.90.","PeriodicalId":136596,"journal":{"name":"Kinetics of Enzymatic Synthesis","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127895455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

From a Sequential to a Continuous Approach for LVV-h7 Preparation during Enzymatic Proteolysis in a Microfluidic- Based Extraction Process 从顺序到连续的方法LVV-h7制备过程中的酶解蛋白在微流体为基础的提取过程

Kinetics of Enzymatic Synthesis Pub Date : 2018-11-12 DOI: 10.5772/INTECHOPEN.80228

Kalim Belhacene, I. Ungureanu, E. Grosu, R. Przybylski, A. Blaga, P. Dhulster, R. Froidevaux

引用次数: 0

Thermodynamic Activity-Based Michaelis Constants 基于热力学活动的米切里斯常数

Kinetics of Enzymatic Synthesis Pub Date : 2018-11-05 DOI: 10.5772/INTECHOPEN.80235

A. Wangler, M. Bunse, G. Sadowski, C. Held

引用次数: 6

Kinetics of Halophilic Enzymes 嗜盐酶动力学

Kinetics of Enzymatic Synthesis Pub Date : 2018-11-05 DOI: 10.5772/INTECHOPEN.81100

L. A. Cira-Chávez, Joseph Guevara-Luna, Marisela YadiraSoto-Padilla, Brenda Román-Ponce, M. Vásquez-Murrieta, M. I. Estrada-Alvarado

引用次数: 7