{"title":"Promising Constructions of Sweet Cherry (Prunus Avium L.) Orchards Under Conditions of Ukrainian Forest-Steppe","authors":"O. Kishchak, Yu. P. Kishchak","doi":"10.15414/agrobiodiversity.2019.2585-8246.294-303","DOIUrl":"https://doi.org/10.15414/agrobiodiversity.2019.2585-8246.294-303","url":null,"abstract":"The purpose of the research was the comparative estimation of the modern sweet cherry (Prunus avium L.) orchards, created according to the foreign and domestic technologies with using the best large-fruited cultivars, high-productive rootstocks, modern methods of the pruning under the optimum dense trees distribution. Field, laboratory and comparative methods of the investigations were applied as well as statistical. The growth processes intensity in the orchards of cv. Regina on Gisela 5 appeared lower than on the middle rootstocks Gisela 6 and VSL-2. Among the pruning methods, the fan-trained crown proved averagely in the research years by 1.2–2.6 times less productive for all the explored cultivar-rootstock combinations as compared to the variants with the rounded small-size and multi-ax flattened crowns. High-productive constructions of the orchards with Talisman and Annushka varieties on the middle rootstocks VSL-2 were selected with a planting plan of 889 trees/ha and rounded small-size and multi-ax flattened crowns. At the age of six years those orchards provide the competitive yield level (8.1–12.5 t/ha) and fruits marketability (94.1–95.7 %). Comparison of the best domestic varieties such as Talisman and Annushka with Regina variety showed that they are competitive and not inferior of foreign ones not only in yield but also in marketable fruit quality. The largest fruits were found out in Talisman variety – 18.4 g with a diameter of 35 mm.The Regina orchards on Gisela 5 and Gisela 6 were low-productive and did not ensure the necessary production marketability level comparing with the Ukrainian orchards.","PeriodicalId":135845,"journal":{"name":"Agrobiodiversity for Improving of Nutrition, Health and Life Quality 2019","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122493978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mineral Composition of Vegetable Biomass of Amaranthus L. Species","authors":"Olena Andrushchenko","doi":"10.15414/agrobiodiversity.2019.2585-8246.224-230","DOIUrl":"https://doi.org/10.15414/agrobiodiversity.2019.2585-8246.224-230","url":null,"abstract":"The mineral composition is explored of the four species of The leaves were analyzed when the plants were in the vegetative phase of development. The element concentrations determined by techniques of atomic absorption spectrometry with inductively coupled plasma spectrometry. The order of the elements is as follows: mg/kg. Maximum content of Mg was 6,734.33, P 1,706.33, Fe 1,133.28 mg/kg. The content of heavy metals was in a safe quantity, except nickel. The highest coefficients of variation (%) are characteristic of the content of barium (45.07), sodium (31.18), vanadium (28.22) and potassium (26.62). The most stable is the content of phosphorus (3.25%). The mineral composition of leaves of amaranth showed its high value as a vegetable product.","PeriodicalId":135845,"journal":{"name":"Agrobiodiversity for Improving of Nutrition, Health and Life Quality 2019","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121773411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Vivodík, E. Saadaoui, L. Petrovičová, Ž. Balážová, Z. Gálová
{"title":"Identification of Tunisian Castor Genotypes (Ricinus Communis L.) Using SCoT Markers","authors":"M. Vivodík, E. Saadaoui, L. Petrovičová, Ž. Balážová, Z. Gálová","doi":"10.15414/agrobiodiversity.2019.2585-8246.249-254","DOIUrl":"https://doi.org/10.15414/agrobiodiversity.2019.2585-8246.249-254","url":null,"abstract":"In the present investigation, 20 genotypes of Tunisian castor were analyzed using 5 start codon targeted (SCoT) markers. These primers produced total 33 fragments across 20 Tunisian castor genotypes, of which 26 (78.70 %) were polymorphic with an average of 5.20 polymorphic fragments per primer and number of amplified fragments ranged from 4 (SCoT 66) to 9 (SCoT 65). The polymorphic information content (PIC) value ranged from 0.652 (ScoT 65) to 0.816 (SCoT 61) with an average of 0.738. The dendrogram based on hierarchical cluster analysis using UPGMA algorithm was prepared. The hierarchical cluster analysis showed that the Tunisian castor genotypes were divided into 3 main clusters. Cluster 1 contained 4 castor genotypes from region Gabes (G-1, G-2, G-4 and G-5). Cluster 2 contained 4 castor genotypes from region Mornag (M-1, M-2, M-4, M-5). Cluster 3 contained 12 Tunisian castor genotypes and was divided into subcluster 3a, 3b, and 3c. Subcluster 3a contained one genotype from region Mornag (M-3), one genotype from region Mednine (MD-5) and 2 genotypes from the region Kebili (K-2 and K-3). Subcluster 3b contained 2 castor genotypes from region Kebili (K-1 and K-5). Subclaster 3c contained five genotypes from region Ksar jedid (KJ-1, KJ-2, KJ-3, KJ-4, KJ-5) and one genotype from region Kebili (K-4). Two Tunisian castor genotypes of 3c subcluster (K-1 nad K-4) were genetically the closest. We can assume that they have a close genetic background. The present study shows the effectiveness of employing SCoT markers in analysis of castor and would be useful for further studies in population genetics, conservation genetics, and genotypes improvement.","PeriodicalId":135845,"journal":{"name":"Agrobiodiversity for Improving of Nutrition, Health and Life Quality 2019","volume":"12 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133041742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Photosynthetic Pigments in Shoots of Vaccinium Corymbosum L. (Cv. Elliott)","authors":"N. Yavorska, N. Vorobets","doi":"10.15414/agrobiodiversity.2019.2585-8246.093-100","DOIUrl":"https://doi.org/10.15414/agrobiodiversity.2019.2585-8246.093-100","url":null,"abstract":"Shoots of Vaccinium corymbosum L. (Ericaceae), which introduced in Ukraine are thought to be responsible for several therapeutic properties. Acetone (80 and 100 %), ethanol (96 %), and dyethyl ether extracts were prepared from aerial parts of V. corymbosum cv. Elliott in order to examine chlorophyll a and b, and carotenoids content. The quantitative content of chlorophyll a and b, and carotenoids in the shoots of V. corymbosum of the Elliott variety has been investigated and the dynamics of their seasonal accumulation, depending on the vegetation phase, has been analyzed. The content of pigments was determined spectrophotometrically at wavelengths that correspond to the absorption maxima of chlorophylls a and b and carotenoids for each solvent. For comparison, Vaccinium uliginosum L. (bog bilberry) shoots were used, as an aboriginal European species of Ericaceae. It was established that the best solvent for extraction of chlorophylls in V. corymbosum shoots is 100 % acetone, as compared to 96 % ethanol and diethyl ether. The highest content of both chlorophylls was in the flowering and fruiting stages. The maximum values for chlorophylls a and b content were recorded in autumn period – end of September-beginning of October (phase III of growing season ): 78.57 ±2.02 mg/100 g DW for chlorophyll a, and 81.13 0.72 mg/100 g DW for chlorophyll b. In the 80% acetone as extragent the maximum values for both chlorophylls were observed in Phase II of fruiting 73.24 ±0.27 mg/100 g DW for chlorophyll a and 48.51 ±0.55 mg/100 g DW for chlorophyll b. In all the variants studied, the minimum value of chlorophylls a and b was observed in the IV winter growing season. The content of chlorophylls in the shoots of V. corymbosum is higher compared to the V. uliginosum during fruiting period, and the total content of carotenoids in the V. corymbosum and V. uliginosum shoots was not significantly different at the fruiting stage.","PeriodicalId":135845,"journal":{"name":"Agrobiodiversity for Improving of Nutrition, Health and Life Quality 2019","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115847819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Possibility of Reproduction of Linum Usitatissimum L. from Seeds with Low Germination and Viability In Vitro Conditions","authors":"S. Mishchenko","doi":"10.15414/agrobiodiversity.2019.2585-8246.304-311","DOIUrl":"https://doi.org/10.15414/agrobiodiversity.2019.2585-8246.304-311","url":null,"abstract":"The development of a method for the reproduction of flax (Linum usitatissimum L.) from seeds of low germination and viability was the goal of our research. To prevent damage to the seed embryo and weak seedlings for sterilization of the seeds aqueous NaOCl solution at a reduced concentration (1.5%) and a reduced exposure (10 minutes) was applied. The effect of a combination of various combinations of phytohormones and other biologically active substances on the germination of flax seed was established. Cultivation of seeds in the Murashige and Skoog medium with macro elements and microelements in the full dose which includes 2.5 mg/L glycine, 0.2 mg/L thiamine, 1.0 mg/L pyridoxine, 5.0 mg/L ascorbic acid, 0.2 mg/L kinetin (КIN), 0.2 mg/L gibberellic acid (GA3), 4.0 m/L succinic acid, 12.5 g/L sucrose and does not contain nicotinic acid increases the seeds germination to 10.0–25.0%. In this case, the explants were cultivated for 2–3 days at a temperature of 19–21 °C and further at a temperature of 22–24 °C. The White medium which contains 0.1 mg/L of 1-naphthylacetic acid (NAA) and 12.5 g/L of sucrose was used for microclonal propagation of the generated shoots. The proposed method provides the obtaining of valuable flax seed breeding material from the seed of low germination and viability, a high coefficient of its reproduction, which accelerates the breeding process.","PeriodicalId":135845,"journal":{"name":"Agrobiodiversity for Improving of Nutrition, Health and Life Quality 2019","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125484739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ukraine Morphological and Anatomical Structural Features of Shoots of Species of the Genus Thymus L., Introduced in Botanical Garden of Lviv National University Named After Ivan Franko","authors":"M. Hrytsyna, M. Skybitska, I. Salamon","doi":"10.15414/agrobiodiversity.2019.2585-8246.038-050","DOIUrl":"https://doi.org/10.15414/agrobiodiversity.2019.2585-8246.038-050","url":null,"abstract":"The aim of work is the exploration of morphological and anatomical features of species of genus Thymus L., which are exhibited at a ‘medical plants’ collection in Вotanical Garden of Ivan Franko National University of Lviv, namely Th. vulgaris L., Th. zygis L., Th. camphoratus Hoffmanns. Link, Th. callieri Borb. Anatomical structure studies of a lamina of leaves were conducted with the help of ‘Leica DM 500’ microscope. Raw of species of genus Thymus is aboveground mass on a stage of flowering. In explored species leaves are contrary crisscrossed, in the lower part of sprout they are small petioled and upwards they become sedentary. The pattern of the lamina is changed in a similar way. In all species, these laminas are wrapped up. Pubescence of leaves is made of simple, non-glandular hairs and glandular essential oils (capitate and peltate) trichomes. Leaves Th. vulgaris are lanceolate, dark green above, wrapped below, where they are gray from dense pubescence with simple, unicellular, curved hairs. Leaves of Th. zygis are grey because of thick pubescence. It is created of a single cell, upwards sharpened hairs, and two-cell curved hairs, where three cellular hairs can be met. In Th. callieri leaves are green, over the edge of lamina they have pubescence of long, 5–7 cellular impacted hairs and between them short, 1–3 cellular hairs are situated. Simple hairs also are situated along veins as well. In all of the species capitate trichomes are situated on the surface of a lamina. Except this, on the surface of lamina essential peltate hairs with multicellular glands are situated. Cells of the upper epidermis of Th. vulgaris and Th. zygis they are oval, Th. callieri has epidermis cells with winding walls. The stomata apparatus is of a diacetate type. The amount of stomata is bigger from the abaxial side than from adaxial surface leaves. Essential oil fittings are situated above big veins in mesophile of a leaf. Their numerous cells contain thick cytoplasmic content. The mesophyll is formed from spongy tissue, which has 1 large and, rarely, 2 small essential oil containers (Th. vulgaris). Other species have 3–5 essential oil fittings. All species formed inflorescence of the compleх tassel. The apex of inflorescences grows monopodially. It is racemous (open). The partial inflorescences are dichasium and","PeriodicalId":135845,"journal":{"name":"Agrobiodiversity for Improving of Nutrition, Health and Life Quality 2019","volume":"122 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128006901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}