Hrc-journal of High Resolution Chromatography最新文献

Prototypical Test Substances for a Reversed Phase in a Retention Parameter Model 保留参数模型中反向相的原型试验物质

Hrc-journal of High Resolution Chromatography Pub Date : 1999-08-01 DOI: 10.1002/(SICI)1521-4168(19990801)22:8<454::AID-JHRC454>3.0.CO;2-#

L. Rohrschneider

引用次数: 9

Use of isopropanol as a modifier in a hexane-acetonitrile based mobile phase for the silver ion HPLC separation of positional isomers of triacylglycerols containing long chain polyunsaturated fatty acids 以异丙醇为改性剂，以正己烷-乙腈为流动相，用银离子高效液相色谱法分离含长链多不饱和脂肪酸的三酰基甘油的位置异构体

Hrc-journal of High Resolution Chromatography Pub Date : 1999-06-01 DOI: 10.1002/(SICI)1521-4168(19990601)22:6<357::AID-JHRC357>3.0.CO;2-#

J. Han, Y. Iwasaki, T. Yamane

{"title":"Use of isopropanol as a modifier in a hexane-acetonitrile based mobile phase for the silver ion HPLC separation of positional isomers of triacylglycerols containing long chain polyunsaturated fatty acids","authors":"J. Han, Y. Iwasaki, T. Yamane","doi":"10.1002/(SICI)1521-4168(19990601)22:6<357::AID-JHRC357>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1521-4168(19990601)22:6<357::AID-JHRC357>3.0.CO;2-#","url":null,"abstract":"A high resolution approach to silver ion HPLC was studied for the separation of positional isomers of triacylglycerols (TAGs) containing long chain polyunsaturated fatty acids (PUFA) such as eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and docosapentaenoic acid (DPA) in enzymatically synthesized structured TAGs. Isopropanol was used as a novel modifier in a hexane-acetonitrile based mobile phase for silver ion HPLC. Peak identification was based on HPLC-mass spectroscopy and selectivities of lipases. Positional isomers of TAGs containing one molecule of EPA, DHA, or DPA with saturated fatty acids (FAs) such as caprylic acid and palmitic acid were separated within 13 min using a gradient of hexane-isopropanol-acetonitrile as mobile phase. TAGs containing two or more EPA, DHA, or DPA were also separated from each other within 25 min, but their positional isomers were unresolved. The retention characteristics of the TAG were found to be related to the number of carbon atoms in the FAs present in addition to the number of double bonds and their isomeric configuration. One isomer with an unsaturated FA in the sn-2 position eluted faster than the other with the unsaturated FA in the sn-1 or 3 position. Species with longer chain FAs attached to TAGs with the same degree of unsaturation eluted faster than those that have shorter chain FAs. For example, docosapentaenoylhexadecanoyloctanoin (DPA/C16/C8) was eluted faster than dioctanoyldocosapentaenoin (DPA/C8/C8).","PeriodicalId":13108,"journal":{"name":"Hrc-journal of High Resolution Chromatography","volume":"109 4 1","pages":"357-361"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83625563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Separation of Chlorophylls and Their Degradation Products using Packed Column Supercritical Fluid Chromatography (SFC) 填充柱超临界流体色谱法分离叶绿素及其降解产物

Hrc-journal of High Resolution Chromatography Pub Date : 1999-06-01 DOI: 10.1002/(SICI)1521-4168(19990601)22:6<339::AID-JHRC339>3.0.CO;2-#

S. Buskov, H. Sørensen, S. Sørensen

引用次数: 6

Comprehensive two-dimensional gas chromatography using a modulating cryogenic trap 综合二维气相色谱使用调制低温阱

Hrc-journal of High Resolution Chromatography Pub Date : 1998-11-01 DOI: 10.1002/(SICI)1521-4168(19981101)21:11<620::AID-JHRC620>3.0.CO;2-#

R. M. Kinghorn, P. Marriott

引用次数: 102

A General Model for the Optimization of Sample Processing Conditions by Solid‐Phase Extraction Applied to the Isolation of Estrogens from Urine 固相萃取法分离尿液中雌激素样品处理条件优化的通用模型

Hrc-journal of High Resolution Chromatography Pub Date : 1998-09-01 DOI: 10.1002/(SICI)1521-4168(19980901)21:9<481::AID-JHRC481>3.0.CO;2-#

D. Seibert, C. Poole

{"title":"A General Model for the Optimization of Sample Processing Conditions by Solid‐Phase Extraction Applied to the Isolation of Estrogens from Urine","authors":"D. Seibert, C. Poole","doi":"10.1002/(SICI)1521-4168(19980901)21:9<481::AID-JHRC481>3.0.CO;2-#","DOIUrl":"https://doi.org/10.1002/(SICI)1521-4168(19980901)21:9<481::AID-JHRC481>3.0.CO;2-#","url":null,"abstract":"A general model is presented for the prediction of experimental conditions for the isolation of organic compounds from aqueous solution by solid-phase extraction. The solvation parameter model is used to predict the variation of retention as a function of solvent composition from characteristic solute descriptors and combined with a frontal analysis model to adjust the predicted breakthrough volumes to account for the influence of the small number of theoretical plates characteristic of sorbent cartridges. System maps are used to determine breakthrough volumes for sample processing, the composition and volume of wash solvents for matrix simplification, and the composition and volume of eluting solvents. System maps are provided for an octadecylsiloxane-bonded silica sorbent with methanol and acetonitrile as cosolvents with water. These maps are used to calculate the experimental conditions for the isolation of estrogens from urine and the model validated by experimental verification of the model predictions. The proposed approach provides a simple theoretical framework for computer-aided method development in solid-phase extraction.","PeriodicalId":13108,"journal":{"name":"Hrc-journal of High Resolution Chromatography","volume":"68 1","pages":"481-490"},"PeriodicalIF":0.0,"publicationDate":"1998-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74113791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

A Chiral Recognition Mechanism Proposed for Resolving π‐Acidic Racemates on π‐Acidic Chiral Stationary Phases Derived from (S)‐Leucine (S) -亮氨酸衍生的π -酸性手性固定相对π -酸性外消旋物的手性识别机制

Hrc-journal of High Resolution Chromatography Pub Date : 1998-08-01 DOI: 10.1002/(SICI)1521-4168(19980801)21:8<464::AID-JHRC464>3.0.CO;2-#

M. Hyun, Young Duk Kim, S. Han, Jung Bae Lee

引用次数: 14

Miniaturized injection method using silver-coated capillaries in DNA sequencing by multiplexed capillary electrophoresis 多路毛细管电泳DNA测序中镀银毛细管的微型化注射方法

Hrc-journal of High Resolution Chromatography Pub Date : 1998-05-01 DOI: 10.1002/(SICI)1521-4168(19980501)21:5<287::AID-JHRC287>3.0.CO;2-#

T. Kaneta, Hyuk Jeong, E. Yeung

引用次数: 4

Qualitative Evaluation of Aromatic Herbs by Direct Head Space (GC)2 Analysis. Applications of the Method and Comparison with the Traditional Analysis of Essential Oils 直接顶空(GC)2法定性评价芳香类药材。该方法的应用及与传统精油分析方法的比较

Hrc-journal of High Resolution Chromatography Pub Date : 1982-04-01 DOI: 10.1007/978-94-009-7642-9_14

F. Chialva, G. Gabri, P. Liddle, Franco Ulian

引用次数: 36

Ion pair chromatography on reversed‐phase layers 反相层上的离子对色谱

Hrc-journal of High Resolution Chromatography Pub Date : 1979-12-01 DOI: 10.1007/BFB0024398

Dieter-Günter Volkmann

引用次数: 7