{"title":"Trauma","authors":"Stephen Topp, T. Wylie, S. Godwin","doi":"10.2307/j.ctvbtzm4c.49","DOIUrl":"https://doi.org/10.2307/j.ctvbtzm4c.49","url":null,"abstract":"DETERMINATION OF ETHYL-GLUCURONIDE AND ETHYL-SULFATE IN BLOOD BY UPLC-MS/MS: CLINICAL APPLICATION IN TRAUMA VICTIMS. We developed a UPLC-MS/MS method for the quantification of the ethanol metabolites ethyl-glucuronide (EtG) and ethyl-sulfate (EtS) in blood samples. Sample preparation was a protein precipitation with acetonitrile 0.1% formic acid containing internal standards (IS) 0.5 µg mL -1 EtG-D5 and EtS-D5. Chromatographic separation was performed in a fluoro-phenyl column, mobile phases were 0.1% formic acid in water (A) and acetonitrile (B) in gradient elution from 5% B to 70% B, with 10 min run time. Ionization was performed with electrospray source at 550 °C in negative mode, quantification mass transitions of m/z EtG 221- 75, EtS 125- 80.1, EtS-D5 130- 79.9, and EtG-D5 226- 75.1. The method was linear from 0.1 to 18 µg mL -1 for EtG and 0.02 to 6 µg mL -1 for EtS, specific, with no interfering peaks during the blank samples analyses. The method was precise and accurate, the matrix effect was compensated with the IS (-12% a + 6.1%). EtG and EtS were analyzed in 28 blood samples from trauma patients with positive alcoholaemia. The concentrations of EtG and EtS ranged from 0.25 to 14.11 µg mL -1 and 0.13 to 5.53 µg mL -1 , with high correlation with ethanol concentrations, r=0.739 and r=0.817 (p <0,001), respectively.","PeriodicalId":130120,"journal":{"name":"Comprehensive Healthcare Simulation: Emergency Medicine","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127605058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Education and Learning Theory","authors":"J. Takayesu, Nelson Wong, James W. Gordon","doi":"10.1007/978-3-030-57367-6_2","DOIUrl":"https://doi.org/10.1007/978-3-030-57367-6_2","url":null,"abstract":"","PeriodicalId":130120,"journal":{"name":"Comprehensive Healthcare Simulation: Emergency Medicine","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132115618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Standardized Participants","authors":"J. Siegelman, S. Nagrani, C. H. Coralli, D. Ander","doi":"10.1007/978-3-030-57367-6_9","DOIUrl":"https://doi.org/10.1007/978-3-030-57367-6_9","url":null,"abstract":"","PeriodicalId":130120,"journal":{"name":"Comprehensive Healthcare Simulation: Emergency Medicine","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116143268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}